Studies on Trypsin Inhibitor in Bean (Phaseolus vulgaris L) Cultivars of Himalayan Region

Biological Activity of Serine Protease Inhibitor Isolated from the Seeds of Phaseolus vulgaris

“…This could be due to inhibition of gut proteases of larvae causing deficiency of essential amino acids. Feeding bioassay with on neonate P. brassicae larvae with trypsin inhibitors from seeds of local yellow cultivar from P. vulgaris and Albizia lebbeck resulted in 57-90% mortality at 67-150-lg concentrations [4,18]. P. brassicae larvae fed on treated leaf discs for 6 days showed reduction in growth during the period of investigation (Table 3, Fig.…”

“…However, trypsin inhibitor was purified to 13.51-fold with 30.53% recovery from seed flour of Vigna radiata L. Wilczek by ammonium sulphate precipitation and gel filtration chromatography on Sephadex G-75. Higher fold purification was achieved from seeds flour of P. vulgaris L., Albizia lebbeck and cowpea (Vigna unguiculata) because ion-exchange chromatography in addition to above mentioned steps for purification was used [4,18,22]. Native-PAGE and SDS-PAGE of partially purified inhibitor protein from Baspa cultivar revealed two protein bands.…”

“…The protein of molecular weight 14,092 daltons was also found to be present in crude extract and ammonium sulphate fraction. The purified trypsin inhibitor protein from seed flour extract of P. vulgaris L. cultivar local yellow was reported to have a molecular weight of 14,400 daltons [4]. Molecular weight of purified Bowman-Birk trypsin inhibitor from P. vulgaris cv.…”

“…Inhibitor protein was extracted from defatted seed flour of P. vulgaris L. cultivar Baspa and trypsin inhibitor activity estimated as described by Deepika and Nath [4]. Soluble protein in the inhibitor extracts was estimated as described by Lowry et al [14].…”