Studies on Tissue Thromboplastin - Its Splitting into Two Separable Parts

Abstract

Summary1. Sodium deoxycholate (DOC) splits tissue thromboplastin into two inactive parts, fraction A and fraction B, separable by gel filtration in the presence of DOC.2. By recombination and subsequent removal of the DOC, the fractions reaggregate and regain full thromboplastic activity.3. Fraction A consists mainly of protein, while phospholipid is the main constituent of fraction B.

Phospholipase C from Bacillus cereus and Its Use in Studies of Tissue Thromboplastin

“…Crude tissue thromboplastin was prepared from human brain [5] and further purified [6]. Both crude and purified thromboplastin were used.…”

Solubilisation von Gewebethromboplastin. Effekt verschiedener Detergentien

“…Es gelang ihnen so, die thromboplastische Aktivität quantitativ in Lösung zu bringen. Daneben war es ihnen mög lich, durch Gelfiltration in Gegenwart des Detergens den Proteinanteil vom Phospholipidanteil des GT zu trennen [7], Nun war der Weg zur weiteren Reinigung des Proteinanteiles offen. Bj0 rklid et al [1] konnten das Protein soweit reinigen, dass in der Polyacrylamidelektrophorese nur mehr eine Bande nachweisbar war.…”

“…Erst durch die Verwendung von Na-Desoxycholat [6] gelang es, Gewebethromboplastin löslich zu machen, die Aktivität aus dem Gewebe zu extrahieren und wei tere Reinigungsschritte durchzuführen. Durch Gelfiltration in Gegenwart des Detergens war es möglich, das Protein vom Phospholipid auf einfache Weise zu trennen [7] und schliesslich rein darzustellen [1].…”

The Influence of the Polyene Filipin on the Extrinsic Pathway of Blood Coagulation

“…Accord ing to several investigators [4,7,10,19,27], thromboplastin can be split up into a lipid part and a protein part. To find out whether the lipid part of thromboplastin is important at this level, the activation of a purified factor VII preparation by bovine or human brain thromboplastin was studied by means of a two-stage clotting test (see Methods, No.…”