Studies on the utilization of citrus peel for pectinase production using fungus<i>Aspergillus niger</i>

Dhillon, Satvinder Singh; Gill, Rajwant Kaur; Gill, Sikander Singh; Singh, Mohit Kumar

doi:10.1080/0020723032000143346

Cited by 66 publications

(33 citation statements)

References 18 publications

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“…Hence, commercial use of orange peel powder for biosynthesis of pectinase can be highly inexpensive, which may trim down the processing cost to a greater extent. Citrus peel was also used for the formulation of fermentation medium by Dhillon et al (2004) for the biosynthesis of pectinase. Employments of such byproducts in industries for the enzyme production in bulk can reduce processing costs as well can bring betterment to the environment and industry.…”

Section: Liquid Static Surface Fermentation Versus Shaking Fermentationmentioning

confidence: 99%

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Sethi¹,

Panda²,

Sahoo³

2014

Asian J. of Biotechnology

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In this study, Aspergillus terreus NCFT4269.10 was selected for the biosynthesis of pectinase after screening from fifteen native fungal strains as the performance of A. terreus was best in pectin hydrolyzing activity (2.75 cm). Aiming at the cost effective fermentation, several agro-industrial residues such as, Pearl Millet (PM), Finger Millet (FM), Orange Peels (OP), Mustard oil Cake (MoC) and Chikling Vetch Peels (CVP) were evaluated for highest pectinase production using Liquid Static Surface Fermentation (LSSF) and Liquid Shaking Fermentation (LShF). Among these substrates, orange peel was found to be the most suitable substrate for optimal pectinase biosynthesis (LSSF: 633.33±57.73 U mLG ) when the culture was grown under static and shaking condition at 30°C for 96 h. Maximum biomass was formed when OP was implemented for fermentation at static condition followed by CVP. But maximum biomass did not always support the enhanced pectinase biosynthesis in most of the cases. Partial purification of pectinase revealed a 3.86 fold purification and 27.63% yield of protein. Fermentation kinetics study revealed that purified enzyme activity yield per gram of substrate was maximum at both LSSF (1866.6 U gdsG 1 ) and LShF (2026.7 U gdsG 1 ) when biosynthesis of pectinase was carried out using OP. Ratio of enzyme yield and biomass were best with OP as compared to the other substrates both in static and shaking fermentation at crude and purified conditions.

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Section: Liquid Static Surface Fermentation Versus Shaking Fermentationmentioning

confidence: 99%

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Sethi¹,

Panda²,

Sahoo³

2014

Asian J. of Biotechnology

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“…Spores of Trichoderma viride were grown and maintained on Potato Dextrose Agar (PDA) slants. Spores of Trichoderma viride from PDA slant were cultivated in an Erlenmeyer flask (250 mL) containing 30 mL of Potato Dextrose broth at 30 ± 1˚C for 7 days after sterilizing the broth at 15 lbs/inch 2 pressure and 121˚C in laboratory scale autoclave (Sanyo, Japan) for 15 minutes, pH was adjusted before sterilization, and incubated under stationary conditions for the development of fungal spore suspension [15].…”

Section: Micro-organism Maintenance and Inoculum Developmentmentioning

confidence: 99%

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Iqbal¹,

Ahmed²,

Zia³

et al. 2011

ABB

108

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This paper reports the purification and characterization of kinetic parameters of cellulase produced from Trichoderma viride under still culture solid state fermentation technique using cheap and an easily available agricultural waste material, wheat straw as growth supported substrate. Trichoderma viride was cultured in fermentation medium of wheat straw under some previously optimized growth conditions and maximum activity of 398 ± 2.43 U/mL obtained after stipulated fermentation time period. Cellulase was purified 2.33 fold with specific activity of 105 U/mg in comparison to crude enzyme extract using ammonium sulfate precipitation, dialysis and Sephadex-G-100 column chromatography. The enzyme was shown to have a relative low molecular weight of 58 kDa by sodium dodecyl sulphate poly-acrylamide gel electrophoresis. The purified enzyme displayed 6.5 and 55˚C as an optimum pH and temperature respectively. Using carboxymethyl cellulose as substrate, the enzyme showed maximum activity (V max ) of 148 U/mL with its corresponding K M value of 68 µM. Among activators/inhibitors SDS, EDTA, and Hg 2+ showed inhibitory effect on purified cellulase whereas, the enzyme activated by Co 2+ and Mn 2+ at a concentration of 1 mM. The purified cellulase was compatible with four local detergent brands with up to 20 days of shelf life at room temperature suggesting its potential as a detergent additive for improved washing therefore, it is concluded that it may be potentially useful for industrial purposes especially for detergent and laundry industry.

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“…According to Roumbouts and Pilnik (1980), culture media with an adequate balance of simple carbohydrates and pectin lead to the best production of pectinases in a submerged process. Due to their role as pectinase inducers, pectin or agricultural residues with high pectin content are essential components of the culture medium (Dhillon et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Influence of pectin, glucose, and pH on the production of endo- and exo-polygalacturonase by Aspergillus oryzae in liquid medium

Fontana

Silveira

2012

Braz. J. Chem. Eng.

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-Endo-and exo-polygalacturonase (PG) production by Aspergillus oryzae IPT 301 was evaluated in a batch bioreactor in media containing 0, 10, and 20 g.L -1 pectin and 0 to 30 g.L -1 glucose. For cultivations in which the pH was not controlled, the use of 20 g.L -1 pectin and 10 g.L -1 glucose resulted in superior enzyme activities compared to control media with 0 and 10 g.L -1 of the inducer and the same glucose concentration. Maximum endo-PG and exo-PG activities were 125.0 and 76.3 U.mL -1 , respectively. For cultivations in which the pH was controlled to a minimum value of 2.7, media containing 20 g.L -1 pectin and 20 to 50 g.L -1 glucose were tested and significant improvement of both activities was attained. Maximum enzyme activities (175.0 U.mL -1 for endo-PG and 103.0 U.mL -1 for exo-PG) were obtained in pH-controlled batch experiments with 30 g.L -1 glucose.

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Studies on the utilization of citrus peel for pectinase production using fungusAspergillus niger

Cited by 66 publications

References 18 publications

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Influence of pectin, glucose, and pH on the production of endo- and exo-polygalacturonase by Aspergillus oryzae in liquid medium

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