Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing

Zhao, Liang; Chen, Denglong; Yao, Qinghua; Li, Min

doi:10.2147/ijn.s47256

Cited by 22 publications

(16 citation statements)

References 20 publications

(16 reference statements)

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“…We have done large amount of similar work on the preparation of PCL/fibrin scaffolds by electrospinning technology [ [20] , [21] , [22] ]. In brief, PCL and fibrin at the weight of 20:80 were mixed thoroughly with 98% formic acid.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Zhao

Lei

et al. 2021

Materials Science and Engineering: C

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The success of artificial vascular graft in the host to obtain functional tissue regeneration and remodeling is a great challenge in the field of small diameter tissue engineering blood vessels. In our previous work, poly(ε-caprolactone) (PCL)/fibrin vascular grafts were fabricated by electrospinning. It was proved that the PCL/fibrin vascular graft was a suitable small diameter tissue engineering vascular scaffold with good biomechanical properties and cell compatibility. Here we mainly examined the performance of PCL/fibrin vascular graft in vivo. The graft showed randomly arranged nanofiber structure, excellent mechanical strength, higher compliance and degradation properties. At 9 months after implantation in the rat abdominal aorta, the graft induced the regeneration of neoarteries, and promoted ECM deposition and rapid endothelialization. More importantly, the PCL/fibrin vascular graft showed more microvessels density and fewer calcification areas at 3 months, which was beneficial to improve cell infiltration and proliferation. Moreover, the ratio of M2/M1macrophage in PCL/fibrin graft had a higher expression level and the secretion amount of pro-inflammatory cytokines started to increase, and then decreased to similar to the native artery. Thus, the electrospun PCL/fibrin tubular vascular graft had great potential to become a new type of artificial blood vessel scaffold that can be implanted in vivo for long term.

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Section: Methodsmentioning

confidence: 99%

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Zhao

Lei

et al. 2021

Materials Science and Engineering: C

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“…34,35 In recent years, material-based drug delivery systems have been used to expand applications of bioactive proteins and peptides, especially in wound healing applications. [36][37][38] Cytokine FGFs were incorporated into composite CMCS hydrogel for accelerating skin regeneration by enhancing skin permeability and avoiding rapid degradation. 18 CMCS-encapsulated insulin showed better outcomes in epithelial permeation, bioavailability, and half-time than single insulin application.…”

Section: Discussionmentioning

confidence: 99%

Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing

Sun¹,

Zhan²,

Zhang³

et al. 2018

IJN

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BackgroundNonscar wound healing is a desirable treatment for cutaneous wounds worldwide. Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.PurposeTo overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.MethodsCMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering. Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC. Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods. The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.ResultsThe release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner. CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes. In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone. Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition. CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.ConclusionThe prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.

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“…Until then, our research group has done a lot of work similar to the preparation of PU/fibrin vascular scaffolds using electrospinning technology. 14–16 In short, we dissolved 15% wt of PU in a tetrahydrofuran (THF)/dimethylformamide (DMF) solution (3:1v/v) and put it in stirring instrument for 24 hours to prepare PU solution. Fibrin with a concentration of 10%wt was dissolved in 98% formic acid solution and stirred for 4 hours to obtain fibrin solution.…”

Section: Methodsmentioning

confidence: 99%

<p>Preparation of PU/Fibrin Vascular Scaffold with Good Biomechanical Properties and Evaluation of Its Performance in vitro and in vivo</p>

Lei

et al. 2020

IJN

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The development of tissue-engineered blood vessels provides a new source of donors for coronary artery bypass grafting and peripheral blood vessel transplantation. Fibrin fiber has good biocompatibility and is an ideal tissue engineering vascular scaffold, but its mechanical property needs improvement. Methods: We mixed polyurethane (PU) and fibrin to prepare the PU/fibrin vascular scaffolds by using electrospinning technology in order to enhance the mechanical properties of fibrin scaffold. We investigated the morphological, mechanical strength, hydrophilicity, degradation, blood and cell compatibility of PU/fibrin (0:100), PU/fibrin (5:95), PU/fibrin (15:85) and PU/fibrin (25:75) vascular scaffolds. Based on the results in vitro, PU/fibrin (15:85) was selected for transplantation in vivo to repair vascular defects, and the extracellular matrix formation, vascular remodeling, and immune response were evaluated. Results: The results indicated that the fiber diameter of the PU/fibrin (15:85) scaffold was about 712nm. With the increase of PU content, the mechanical strength of the composite scaffolds increased, however, the degradation rate decreased gradually. The PU/fibrin scaffold showed good hydrophilicity and hemocompatibility. PU/fibrin (15:85) vascular scaffold could promote the adhesion and proliferation of mesenchymal stromal cells (MSCs). Quantitative RT-PCR experimental results showed that the expression of collagen, survivin and vimentin genes in PU/fibrin (15:85) was higher than that in PU/fibrin (25:75). The results in vivo indicated the mechanical properties and compliance of PU/fibrin grafts could meet clinical requirements and the proportion of thrombosis or occlusion was significantly lower. The graft showed strong vasomotor response, and the smooth muscle cells, endothelial cells, and ECM deposition of the neoartery were comparable to that of native artery after 3 months. At 3 months, the amount of macrophages in PU/fibrin grafts was significantly lower, and the secretion of pro-inflammatory and anti-inflammatory cytokines decreased. Conclusion: PU/fibrin (15:85) vascular scaffolds had great potential to be used as smalldiameter tissue engineering blood vessels.

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Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing

Cited by 22 publications

References 20 publications

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing

<p>Preparation of PU/Fibrin Vascular Scaffold with Good Biomechanical Properties and Evaluation of Its Performance in vitro and in vivo</p>

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