In a recent investigation in these laboratories dogs of various breeds and body-weight, and of either sex, were maintained in metabolism cages on a standard diet and standard water intake and their urinary excretion of thiamine was measured (Worden, Waterhouse & Partington, 1954). It was found that, when under these conditions, the environmental temperature was increased by approximately 30' F, the volume of urine decreased to approximately one-third, and that, although the concentration of thiamine in this reduced urine volume was greater, there was a significant drop in its total daily urinary excretion.During previous investigations with dogs in metabolism cages under standard conditions, it had been noted on a number of occasions that the urinary excretion of riboflavin varied with environmental temperature. During experiments in which environmental temperature was not controlled, whenever the atmospheric temperature became warmer there was a corresponding daily increase in urinary riboflavin output, suggesting a lower requirement at the higher temperatures. It was therefore decided to investigate this temperature effect further.
EXPERIMENTALThe observations were made on three dogs (two male, one female) accommodated in metabolism cages; samples were collected as previously described. The identification numbers of the dogs employed in the previous studies have been retained (Worden et al. 1952, 1954).The riboflavin contents of the urine were determined in volumes containing approximately I 5 pg ; the samples were acidified with 0.2 ml. hydrochloric acid