The purpose of this investigation was to determine whether or not larval tissues of Xenopus laenis are capable of responding to a very short exposure of 3,5,3'-triiodo-L-thyronine (T& very early in their ontogeny. Ten dejellied embryos were immersed in 15 ml of aged tap water containing 30 pg of TB per ml of medium. Exactly at the end of 24 hours all of the embryos were removed, rinsed thoroughly in fresh tap water and placed in aged tap water i n a larger container. Both gross morphological and histological observations were carried out on embryos and larvae at a n interval of 12 hours beginning a t the termination of hormone treatment to the end of about 11 days.The following changes were clearly observed in experimental animals (numbers in parenthesis indicate days after fertilization): (a) degenerative changes i n adhesive gland (3.5); (b) more extensive development of nasal sac (5.0); (c) a visible and ventrally protruding hyoid arch (6.5); (d) a larger hind limb bud (5.0). In addition to the above, other obvious changes which were seen i n experimental animals were a protruding lower jaw due to a n extensive and well developed Meckel's cartilage, and a less extensive gut. The head was cone shaped i n Ta treated animals. There was no change in tail to body length ratios.The facts mentioned above appear to indicate a differential ontogenetic competence of embryonic and larval tissues of Xenopus in their response to TB.