Studies on the Transposable Element Tyl of Yeast: I. RNA Homologous to Tyl

Elder, Robert E.; John, Thomas P. St.; Stinchcomb, Dan T.; Davis, Ronald W.

doi:10.1101/sqb.1981.045.01.075

Cited by 161 publications

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“…In contrast to the SPT3 locus, the MAT locus had a quantitative effect on Ty transcription that reduced the level of total Ty transcript in a/a cells to 1/20 the level seen in haploids or a/a or ala diploids (11). Similarly, a large reduction in the level of Tyl-588NEO transcript was seen in the MATa/a diploid by Northern analysis (Fig.…”

Section: Resultsmentioning

confidence: 90%

“…Regulation of Tyl-588NEO transcription. Previous work has demonstrated that transcription of Ty elements in yeast cells is under control of the SPT3 locus (45,47) and the MAT locus (11,12). To determine whether Tyl-588NEO transcription is regulated by the SPT3 gene, we constructed an isogenic spt3-101 derivative of the Tyl-588(G)NEO strain DG733.…”

Section: Resultsmentioning

confidence: 99%

“…Transcriptional regulation by MAT and the SPT3 gene have been characterized in detail. These studies have shown that there is a 20-fold decrease in total Ty RNA in a/a cells compared with a or a cells (11). In spt3 mutants, full-length Ty transcripts are abolished, and the only Ty transcripts present are initiated 800 bp downstream of the wild-type start site (47).…”

mentioning

confidence: 99%

“…RNA from these elements is very abundant, accounting for an estimated 5 to 10% of total polyadenylated RNA in the haploid cell (11). Ty transposition, on the other hand, occurs at a very low frequency, approximately 10-to 10-8 per gene per generation (4,18,30,36).…”

mentioning

confidence: 99%

“…Total cellular Ty transcription is regulated by several host genes, including MAT, SPT, ROC, and STE genes (7,10,11,45). Transcriptional regulation by MAT and the SPT3 gene have been characterized in detail.…”

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confidence: 99%

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Transpositional competence and transcription of endogenous Ty elements in Saccharomyces cerevisiae: implications for regulation of transposition.

Curcio¹,

Sanders²,

Garfinkel³

1988

Mol. Cell. Biol.

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Transposition of Ty elements in the yeast Saccharomyces cerevisiae occurs through an RNA intermediate. Although Ty RNA accounts for 5 to 10% of the total polyadenylated RNA in a haploid cell, the transposition frequency is only i0' to 10-8 per gene. To determine whether Ty elements native to the yeast genome are transpositionally competent, two elements were fused to the GALI promoter and tested for their ability to transpose. These native elements, Tyl-588 and Ty2-117, transposed at high levels when the GAL) promoter was induced. Three Ty's identified as spontaneous transpositions in specific target genes were also tested. Of these three, Ty2-917 and the previously characterized element Tyl-H3 were shown to be transpositionafly competent. The third element, Tyl-Hi, was transposition defective. In addition, we marked the chromosomal copy of Tyl-588 with the NEO gene and demonstrated that Tyl-588NEO was actively transcribed in yeast cells. Tyl-588NEO transcription was regulated by the SPT3 and MAT loci in the same manner as that observed for Ty's collectively. These results indicate that the yeast genome contains functional Ty elements. The presence of a transpositionally competent, actively transcribed element suggests that regulation of Ty transposition occurs at a posttranscriptional level.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Transpositional competence and transcription of endogenous Ty elements in Saccharomyces cerevisiae: implications for regulation of transposition.

Curcio¹,

Sanders²,

Garfinkel³

1988

Mol. Cell. Biol.

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The yeast Ty element: Recent advances in the study of a model retro‐element

Adams

Kingsman

1987

BioEssays

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SummaryThe past three years have seen a dramatic increase in our understanding of the structural organization and expression strategies of the dispersed, repetitive yeast transposon, Ty. These studies have led to a logical comparison of Ty with retroviral proviruses and other mobile, repetitive elements. Such comparisons have culminated in the hypotheses that transposition occurs via the formation of Ty-encoded virus-like particles and that these particles represent a basic unit of all 'retro-systems'.

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Expression of the ROAM mutations in Saccharomyces cerevisiae: involvement of trans-acting regulatory elements and relation with the Ty1 transcription.

Dubois¹,

Jacobs²,

Jauniaux³

1982

The EMBO Journal

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The regulatory mutations in Saccharomyces cerevisiae designated cargA + Oh, cargB + Oh, and durOh are alterations in the control regions of the respective structural genes. The alteration causing the cargA + Oh mutation has been shown to be an insertion of a Ty1 element in the 5′ noncoding region of the CAR1 (cargA) locus. All three mutations cause overproduction of their corresponding gene products and belong to the ROAM family of mutations (Regulated Overproducing Allele responding to Mating signals) in yeast. The amount of overproduction in ROAM mutants is determined, at least in part, by signals that control mating functions in yeast. We report the identification of two genetic loci that regulate Oh mutant gene expression but that do not affect mating ability. These loci are defined by the recessive roc mutations (ROAM mutation control) that reduce the amount of overproduction caused by the cargA + Oh, cargB + Oh, and durOh mutations. RNAs homologous to CAR1 (cargA), DUR1,2 and Ty1 DNA probes were analyzed by the Northern hybridization technique. In comparison with wild‐type strains, cargA + Oh and durOh mutant strains grown on ammonia medium contain increased amounts of CAR1 and DUR1,2 RNA. This RNA overproduction is diminished in MATa/MAT alpha diploid strains as well as in haploid strains that also carry the ste7 mutation which prevents mating or that carry either of the roc1 or roc2 mutant alleles. The amount of RNA homologous to Ty1 DNA is also reduced in ste7, roc1, and roc2 mutant strains. This reduction is not observed in a strain with the ste5 mutation, which prevents mating but has no effect on overproduction of ROAM mutant gene products.(ABSTRACT TRUNCATED AT 250 WORDS)

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Studies on the Transposable Element Tyl of Yeast: I. RNA Homologous to Tyl

Cited by 161 publications

References 0 publications

Transpositional competence and transcription of endogenous Ty elements in Saccharomyces cerevisiae: implications for regulation of transposition.

Transpositional competence and transcription of endogenous Ty elements in Saccharomyces cerevisiae: implications for regulation of transposition.

The yeast Ty element: Recent advances in the study of a model retro‐element

Expression of the ROAM mutations in Saccharomyces cerevisiae: involvement of trans-acting regulatory elements and relation with the Ty1 transcription.

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