“…alcohol was carried out on an electric water bath with reflux conideniser.5 The accumulated alcohol extracts were evaporated to dryness, the residue dissolved in water and made up to standard volume. On aliquot parts of this solution the total nitrogenl (alcohol soluble N) was determiined by micro-Kjeldahl methods (24) and, after clearing6 with neutral lead acetate, deleading with oxalic acid (17), and acid (3 per cent.HCl) iniversion, the total sugar was determined by the MUNSON-WALKER gravimetric method (6).(3) The residue from alcohol extraction was dried, weighed, and used for the determinatioin of alcohol insoluble nitrogen (proteini N) and starch.The starch was digested with a salivary extract under toluene, alcohol extracted, the soluble products of hydrolysis inverted, the sugar determined by the MUNSON-WALKER method, and the result calculated in terms of glucose (2,3,23,42).A full record of the fate of the carbohydrates during the metabolism of potato discs will be given in a subsequent paper. It is necessary only to remark here that discs, cut from dormant tubers and washed in running tap water for 24 hours, commonly have a total sugar content of about 3.0 mg. per gm.…”