Studies on the Transport and Life‐Span of Amine Storage Granules in a Peripheral Adrenergic Neuron System¹

Abstract

DAHLSTROM, A. and J. HAGGENDAL, Studies on the transport and life-span of amine storage granules in a peripheral adrenergic neuron system. Acta physiol. scand. 1966. 67. 2 78-288. The accumulation of noradrenaline (NA) above a ligation of the rat and cat sciatic nerve and the NA content of the adrenergic fibre system derived from this nerve were estimated. From the data obtained the rate of transport of amine storage granules and their life-span were calculated. For the rat the rate of transport was found to be 5-6 mm per hr and the life-span to be about 35 days. The corresponding values for the cat was 9-10 mm per hour and about 70 days, respectively. HILLARP, N.-A., K. FUXE and A. DAHLSTROM, Central monoamine neurons. Symposium on "Release of Biogenic Amines", Stockholm, February 1965. pp. 31-57. Eds. U. S. v. Euler, S. Rose11 and B. UvcL. Pergamon Press. HAGGENDAL, J., An improved method for fluorimetric determination of small amounts of adrenaline and noradrenaline in plasma and tissues. Acta physiol. scand. 1963. 59. 242-254. KOENIG, E., Synthetic mechanisms in the axon. 11. RNA in myelin-free axons of the cat. 3. Neurochem. 1965. 12. 357-361. LUBINSKA, L., S. NIEMIERKO, B. ODERFELT, L. SZWARC and .J. ZELENA, Bidirectional movements of axoplasm in peripheral nerve fibres. Acta Biol. exp. (Wars.) 1963. 23. 239-247. LUNDAORC., P., Storage function and amine levels of the adrenal medullary granules a t various intervals after reserpine treatment. Experientia 1963. 1.9. 479. MALMPORS, T., Studies on adrenergic nerves. The use of rat and mouse iris for direct observations on their physiology and pharmacology at cellular and subcellular levels. A d a phvsiol. scand. 1965. 64. Suppl. 248. SEDVALL, G., Noradrenaline storage in skeletal muscle. A d a phjwiol. scand. 1964. 60. 39--50. WF.ISS, P., The concept of perpetual neuronal growth and proximo-distal substance convection. In : Re,cional Neurochemisty, 1961. pp. 220-242. Eds.: S. S. Kety and J. Elkes, New-York-London. Pergamon Press.

Rapid Axoplasmic Transport of Tyrosine Hydroxylase in Relation to Other Cytoplasmic Constituents

“…2). Flow rates of 2.6 mm/hr (norepinephrine), 3.4 mm/hr (dopamine-,3-hydroxylase), and 1.9 mm/hr (tyrosine hydroxylase) were calculated by the method of Dahlstrom and Haggendahl (20), which assumes that the accumulation of norepinephrine, dopamine-,3-hydroxylase, and tyrosine hydroxylase activity is solely derived from axoplasmic transport.…”

Structure of the Synapse

“…Bittner & Kennedy (21) calculated how much membrane peripheral nerve terminals would need if they had to produce a new vesicle for each quantum discharged, and it seemed clearly beyond the realm of possibility. Dahlstrom & Haggendal (60) determined, from their measurements of the rate at which vesicles accumulate above axon ligatures, that the supply of new vesicles from the cell body to adrenergic neuromuscular junctions is far too slow to maintain the rate of transmitter discharge and turnover these terminals display. All these studies concluded that vesicle membrane must be conserved after transmitter discharge and reused.…”

Isolation, Reactivity and Aggression: Evidence for an Involvement of Brain Catecholamines and Serotonin

“…The amine granules are known to be transported distally to the nerve terminals by axoplasmic flow (Dahlstrom & Haggendal, 1966;Kapeller & Mayor, 1967;Laduron & Belpaire, 1968). Hence, this pattern of loss of catecho1amines from the area of the brain where the nerve cell bodies are located, with lesser reductions or even increases in concentration in the regions containing the nerve terminals, suggests to us that the intense activation caused by fighting may have accelerated the movement.of catecholamine-containing granules from the cell bodies where they are synthesized towards the nerve terminals where presumably the amines are released at an increased rate during periods of intense activation.…”