Studies on the thyroidal UDPG pyrophosphorylase: Partial purification and some of its properties

Purification, properties and kinetic analysis of UDP-glucose pyrophosphorylase from bovine mammary tissue

“…Purification UDP-glucose pyrophosphorylase has been purified from ~· coli (60) , yeast (Saccharomyces fragilis) (12), mung beans (61), bovine (31,54,62), rat (53,63), rabbit (64), guinea pig (53), dog (7), and human (7,30,55).…”

“…The organs used in mammalian systems are liver (7,30,31,53), muscle (64), heart (7), erythrocytes (7), brain (53,55), thyroid (62), and the mammary gland (54,63). Table I constants and therefore the specific activity.…”

“…could be stored at -20°c for sever~l months with only a gradual loss of activity (60). Bovine thyroid enzyme is more stable in neutral or slightly acid solutions (62), but the enzyme" from §_. coli B is denature<;l by acetate buffers at pH 4 .5 to 6 .o (14).…”

“…Rodriguez et ~· (62) found that the heat stability of the thyroid enzyme was a function of ammonium sulfate concentration. The enzyme was heated at ss 0 c for 4 minutes at various concentrations of ammonium sulfate.…”