Studies on the testicular effects of vitamin A palmitate in the Sprague-Dawley rat

Bosakowski, Thomas; Levin, Arthur A.; Edgcomb, John H.

doi:10.1016/0278-6915(88)90212-8

Cited by 2 publications

(7 citation statements)

References 11 publications

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“…These results are in contrast to those from a previous study (Bosakowski et al, 1988) in which rodents on VAE diet, receiving a twofold higher dose of vitamin A than that used in the present study, showed only reduction in body weight and no reduction in absolute weight of testis relative to the reduction in body weight. Additionally, in the previous study (Bosakowski et al, 1988), 5 to 18-week-old rodents were fed VAE diet for a period of 13 weeks, whereas in our study 3 to 10-week-old mice were fed VAE diet for a period of 7 weeks. It was likely that the effect of VAE toxicity on spermatogenesis was more severe when exposed to VAE at a younger age than that at an older age.…”

Section: Discussioncontrasting

confidence: 99%

“…The present study was conducted to investigate whether long-term intake of VAE arrested spermatogenesis in mice, and it demonstrated for the first time the molecular mechanism of spermatogenic arrest, which was partly different from that in vitamin A-deficient mice as reported previously (Boucheron-Houston et al, 2013). The vitamin A intake (1,000 IU/g diet) in the present study was lesser than in previous reports (Bosakowski et al, 1988) and could not cause teratogenicity (10,000 IU/day) (Miller et al, 1998).…”

Section: Discussioncontrasting

confidence: 58%

“…Vitamin A has long been recognized to be essential for spermatogenesis using vitamin A-deficient rodent models (Wolbach and Howe, 1925;Mitranond et al, 1979;Sobhon et al, 1979;Huang et al, 1988;Morales and Cavicchia, 2002), and acute administration of retinol or retinoic acid has been shown to re-initiate spermatogenesis in vitamin A-deficient rodents (van Pelt and de Rooij, 1990bRooij, , 1991. However, although there is a small amount of evidences about the long-term effects of VAE on rat spermatogenesis, previous studies focused on the effects on body and reproductive organ weights, which have not been previously well characterized (Lamano Carvalho et al, 1978;Bosakowski et al, 1988). The present study was conducted to investigate whether long-term intake of VAE arrested spermatogenesis in mice, and it demonstrated for the first time the molecular mechanism of spermatogenic arrest, which was partly different from that in vitamin A-deficient mice as reported previously (Boucheron-Houston et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…While most studies describe the effects of vitamin A deficiency on spermatogenesis in adult rodents, the role of long-term intake of an excess-vitamin A diet (VAE) in mouse spermatogenesis has not been well investigated. In fact, there were few studies about the long-term effects of VAE on spermatogenesis using rat models, but they only showed the effects of VAE on body and reproductive organ weights (Lamano Carvalho et al, 1978;Bosakowski et al, 1988). Further characterization is needed toward understanding the precise mechanism of male reproductive toxicity of long-term VAE consumption.…”

Section: Introductionmentioning

confidence: 99%

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Long-term dietary intake of excessive vitamin A impairs spermatogenesis in mice

et al. 2019

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Vitamin A and its derivatives contribute to many physiological processes, including vision, neural differentiation, and reproduction. Vitamin A deficiency causes early cessation of spermatogenesis, characterized by a marked depletion of germ cells. However, there has been no clear understanding about the role of chronic intake of vitamin A excess (VAE) in spermatogenesis. The objective of this study was to investigate whether chronic intake of VAE diet causes arrest of spermatogenesis. To examine the effects of VAE on spermatogenesis, we used ICR male mice fed with control (AIN-93G purified diet: 4 IU/g) diet or VAE (modified AIN-93G diet with VAE: 1,000 IU/g) diet for 7 weeks (from 3 to 10 weeks of age). At 10 weeks of age, the retinol concentration in the testes of VAE mice was significantly higher than that of control mice. Testicular cross sections from control mice contained a normal array of germ cells, while the seminiferous tubules from VAE mice exhibited varying degrees of testicular degeneration. Daily sperm production in VAE testes was dramatically decreased compared to that in control testes. Sperm viability, motility, and morphology were also impaired in VAE mice. Furthermore, we examined the effects of VAE on the expression of genes involved in retinoid signaling and spermatogenesis to determine the underlying molecular mechanisms. Therefore, we are the first to present results describing the long-term dietary intake of VAE impairs spermatogenesis using a mouse model.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Long-term dietary intake of excessive vitamin A impairs spermatogenesis in mice

et al. 2019

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“…Then, the VA content of 250 IU/g in the diet, 3.75 times the upper tolerable limit for VA, was used in this study. In rodents, 250 IU/g VA is the lowest VA concentration used in previous studies to examine male reproductive toxicity 16,28,29 as described above. In fact, the dose of VAE used in these studies was too high to evaluate whether VAE actually affects the levels of serum testosterone, since VAE caused a significant body weight reduction by decreasing the appetite as a side effect.…”

Section: Discussionmentioning

confidence: 99%

Impact of chronic vitamin A excess on sperm morphogenesis in mice

et al. 2021

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Background:The increasing availability of fortified foods and supplements has caused an overconsumption of vitamin A (VA), above the recommended level. To date, the effects of chronic VA excess (VAE) on spermatogenesis remain unclear.Objective: This study aims to investigate the long-term excessive intake of VA effects on spermatogenesis in mice. Materials and methods:Dams were initially fed a control diet (4 IU/g) or a VAE diet (250 IU/g), 4 weeks prior to mating and during pregnancy. Dams and their male pups continued this diet regimen until the offspring reached 12 weeks of age. At 12 weeks of age, epididymis caudal spermatozoa and testes were collected. For histological analysis, sections were stained with periodic acid-Schiff-hematoxylin, and quantitative PCR was used to detect changes in gene expression in the testes of the VAE mice. Sperm motility and morphology were evaluated to detect the endpoint of VAE toxicity.Results: Body weights were not significantly different between the control and VAE groups. Testicular cross-sections from the control and VAE mice contained a normal array of germ cells, and the daily sperm production was similar between the two groups. However, the percentage of seminiferous tubules in stages VII and VIII was significantly lower in the VAE mice than in the control. In addition, significant changes in the expression of genes involved in retinoid metabolism, spermatogenesis, and spermiogenesis were detected in the testes of the VAE mice. Consistently, sperm motility and head morphology were significantly impaired in the VAE mice. Discussion and Conclusion:Our findings suggest that long-term dietary intake of VAE was able to influence both pre-and post-meiotic spermatogenesis. As a result of testicular toxicity, we demonstrated, to the best of our knowledge, for the first time that long-term VAE caused sperm-head abnormalities.

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Studies on the testicular effects of vitamin A palmitate in the Sprague-Dawley rat

Cited by 2 publications

References 11 publications

Long-term dietary intake of excessive vitamin A impairs spermatogenesis in mice

Long-term dietary intake of excessive vitamin A impairs spermatogenesis in mice

Impact of chronic vitamin A excess on sperm morphogenesis in mice

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