Studies on the susceptibility of the mouse preimplantation embryo to infection with cytomegalovirus

Neighbour, P. Andrew

doi:10.1530/jrf.0.0540015

Cited by 27 publications

(10 citation statements)

References 12 publications

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“…Early stage mouse embryos are not permissive for MCMV infection; i.e., even if HCMV enters the host cell, it is unable to complete replication cycles [4]. Mouse embryos infected prior to implantation develop normally to the blastocyst stage [5]. If mouse embryos are infected with MCMV at mid-gestation, viral-susceptible cells are first detected in the placenta, followed by blood cells, endothelial and mesodermal cells.…”

Section: Introductionmentioning

confidence: 99%

Human Induced Pluripotent Stem Cell-Derived Models to Investigate Human Cytomegalovirus Infection in Neural Cells

et al. 2012

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Human cytomegalovirus (HCMV) infection is one of the leading prenatal causes of congenital mental retardation and deformities world-wide. Access to cultured human neuronal lineages, necessary to understand the species specific pathogenic effects of HCMV, has been limited by difficulties in sustaining primary human neuronal cultures. Human induced pluripotent stem (iPS) cells now provide an opportunity for such research. We derived iPS cells from human adult fibroblasts and induced neural lineages to investigate their susceptibility to infection with HCMV strain Ad169. Analysis of iPS cells, iPS-derived neural stem cells (NSCs), neural progenitor cells (NPCs) and neurons suggests that (i) iPS cells are not permissive to HCMV infection, i.e., they do not permit a full viral replication cycle; (ii) Neural stem cells have impaired differentiation when infected by HCMV; (iii) NPCs are fully permissive for HCMV infection; altered expression of genes related to neural metabolism or neuronal differentiation is also observed; (iv) most iPS-derived neurons are not permissive to HCMV infection; and (v) infected neurons have impaired calcium influx in response to glutamate.

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Section: Introductionmentioning

confidence: 99%

Human Induced Pluripotent Stem Cell-Derived Models to Investigate Human Cytomegalovirus Infection in Neural Cells

et al. 2012

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“…Cytopathic bovine viral diarrhea virus, blue tongue virus, and bovine herpesvirus-1 have adverse influence on early embryonic development [48][49][50], whereas cytomegalovirus, noncytopathic bovine viral diarrhea virus, and caprine arthritis-encephalitis virus have no impact on development [48,51,52]. No effect of PCV2 replication on in vitro development of ZP-free embryos was observed during the 48-hour study period.…”

Section: Discussionmentioning

confidence: 99%

Damage of zona pellucida reduces the developmental potential and quality of porcine circovirus type 2–infected oocytes after parthenogenetic activation

Zhao

et al. 2014

Theriogenology

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“…In addition, groups of embryos from IVF with CDOs (n ¼ 3, 4,6,10,20,20,20,20,20) or with CEOs (n ¼ 4, 4, 7, 10, 10,10,20,20,20,20,20) were analyzed for the presence of MMVp using PCR and IVIA. Before DNA extraction and PCR as already described for the media, embryos were frozen and thawed three times to allow release of the virus.…”

Section: Virological Examination Of Washing Drops and Embryosmentioning

confidence: 99%

“…The intact zona pellucida (ZP) is regarded as a mechanism in preventing pathogen transmission during ET [4,[10][11][12][13][14][15][16]. Furthermore, the International Embryo Transfer Society recommends that embryos be washed 10 times before transfer to recipients [17].…”

Section: Introductionmentioning

confidence: 99%

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Mahabir¹,

Bulian²,

Needham³

et al. 2009

Biology of Reproduction

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The risk of transmission of mouse minute virus (MMV) to recipients of murine embryos arising from in vitro fertilization (IVF) of cumulus-enclosed oocytes (CEOs) or without cumulus cells (CDOs) in the presence of MMV-exposed (10(4) TCID(50) [mean tissue culture infective dose]/ml MMVp [prototype strain of MMV]) spermatozoa was evaluated. Also, the time after embryo transfer to detection of MMV antibody and the presence of MMV DNA in the mesenteric lymph nodes of recipients and pups were investigated. All mice were MMV free, but two seropositive recipients and four seropositive pups were found in the group with CDOs. With regard to the CEOs, two of 11 holding drops and five of 11 groups of embryos were MMV positive using PCR, while neither holding drops nor embryos carried infectious MMVp, as evidenced by the in vitro infectivity assay. From IVF with CDOs, five of 14 holding drops and four of nine groups of embryos were MMV positive, while one of 14 holding drops and no embryos carried infectious MMVp. When 10(5) cumulus cells were analyzed 5 h after exposure to 10(4) TCID(50)/ml MMVp, cells had an average titer of 10(4) TCID(50)/ml MMVp. The present data show that, in contrast to CDOs, 2-cell embryos from CEOs did not transmit infectious MMVp to the holding drops and to recipients. This observation is due to the presence of cumulus cells during the IVF process that reduce entry of MMV into the zona pellucida and absorb some of the virus. These data further confirm the efficacy of the IVF procedure in producing embryos that are free of infectious virus, leading to virus-free seronegative recipients and rederived pups.

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Studies on the susceptibility of the mouse preimplantation embryo to infection with cytomegalovirus

Cited by 27 publications

References 12 publications

Human Induced Pluripotent Stem Cell-Derived Models to Investigate Human Cytomegalovirus Infection in Neural Cells

Human Induced Pluripotent Stem Cell-Derived Models to Investigate Human Cytomegalovirus Infection in Neural Cells

Damage of zona pellucida reduces the developmental potential and quality of porcine circovirus type 2–infected oocytes after parthenogenetic activation

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

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