KeywordsRibonucleotide, (3-morpholinopropyl)silylsilica gel, 5'-inosinic acid, 5'-xanthylic acid, 5'-guanylic acid, high performance liquid chromatography, seasoning, spectrophotometric detection The mobile phase used in the analyses was consistently an aqueous phosphate buffer.
ExperimentalMaterial MPS was prepared as described in the previous report4by the reaction of silica gel (Daiso gel SP 120, particle size
Results and DiscussionIn the eluent system (0.07 M phosphate buffer), the effect of pH (6.0 -7.5) was tested in order to separate the three components (5'-IMP, 5'-GMP and 5'-XMP). As shown in Fig. 1, the use of 0.07 M phosphate buffer (pH 6.0) containing 10 mM Bu4NBr gave an excellent