Studies on the sorption behavior of quercetin, phenol, and caffeine as test substances on diol mixed-mode HILIC column

Kamińska, J.; Chutkowski, Marcin; Zapała, Lidia; Zapała, Wojciech

doi:10.1080/10826076.2016.1141364

Cited by 7 publications

(19 citation statements)

References 33 publications

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“…Similar adsorption behavior can be also found in systems with diol‐type polar stationary phase (Dionex/Thermo Scientific Acclaim® Mixed‐Mode HILIC‐1 column, 150 × 4.6 mm, 5 μm particle size, 120 Å pore size and 300 m 2 /g surface area) [12]. The AEDs of Q and PH for ACN–water and MeOH–water mobile phases (containing both 70% v/v and 90% v/v of organic solvent) presented in work [12] were found to be bimodal with the main role of low‐energy active sites (type 1). The higher energy interactions (type 2) have in these systems much smaller capacity, q s2 .…”

Section: Resultsmentioning

confidence: 53%

“…In these systems, the AEDs were found to be bimodal and quantitatively very similar as for Q and PH (i.e. the major part of the global adsorption capacity, q s , of the column is due to the sites of type 1 and the sites of type 2 have a smaller capacity) [12]. Regardless of some differences, the obtained results indicate that despite the presence of heterogeneous AEDs in the studied systems, the main role in the adsorption/retention mechanism in amine, amide, zwitterionic, and also diol type adsorbents is played by low‐energy active sites.…”

Section: Resultsmentioning

confidence: 99%

“…As a consequence, the analytes with hydrogen‐bonding potentials similarly interact with the stationary phase regardless the MeOH concentration. Slightly different, but interesting sorption behavior can be observed during tests of the diol column for CAF [12]. In this case, the retention of CAF is probably controlled by active adsorption sites that are located on the stationary phase surface with a low density [12].…”

Section: Resultsmentioning

confidence: 99%

“…For example, in Ref. [12] the presence of both energetically homogeneous and heterogeneous adsorption interactions between an analyte and the tested stationary phase has been found for a diol‐type column packing (neutral stationary phase), and different test substances and mobile phases. These premises prompted the authors to work on research in which the mechanisms of the sorption process of quercetin (Q), phenol (PH), and caffeine (CAF) were analyzed using three chromatographic columns packed with sorbents based on silica gel of very high purity.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of adsorption energy distribution in selected hydrophilic‐interaction chromatography systems with amide, amine, and zwitterionic stationary phases

Ziobrowski

Chutkowski

Przywara

et al. 2021

J of Separation Science

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Adsorption mechanisms of caffeine, quercetin, and phenol as test substances in various chromatographic systems have been analyzed. The investigations were conducted using three different chromatographic columns packed with polar bonded stationary phases, that is, amide, amine, and zwitterionic. Methanol–water and acetonitrile–water systems with different organic solvent contents have been used as mobile phases. On the basis of adsorption isotherms obtained for the tested systems, Scatchard plots and adsorption energy distributions have been determined. The most likely retention mechanisms have been discussed. The results of investigations indicate that (i) the surfaces of tested adsorbents are energetically heterogeneous, and (ii) the main role in sorption mechanism is played by low‐energy sites.

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of adsorption energy distribution in selected hydrophilic‐interaction chromatography systems with amide, amine, and zwitterionic stationary phases

Ziobrowski

Chutkowski

Przywara

et al. 2021

J of Separation Science

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“…To systemically investigate the retention behavior of quercetin, phenol, caffeine on diol Acclaim Mixed Mode HILIC column, three aspects were considered: temperature investigation with van't Hoff plots, column efficiency under different MP systems calculated by van Deemter plots, and adsorption study analyzed by frontal analysis combined with adsorption energy distributions . The temperature investigations showed that except for some systems containing quercetin, the global adsorption behavior was exothermic.…”

Section: Retention Mechanism and Retention Behavior In Hilicmentioning

confidence: 99%

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Zhang

et al. 2016

J of Separation Science

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Hydrophilic interaction liquid chromatography, an alternative liquid chromatography mode, is of particular interest in separating hydrophilic and polar ionic compounds. Compared with traditional liquid chromatography techniques, hydrophilic interaction liquid chromatography offers specific advantages mainly including: (1) relatively green and water-soluble mobile phase composition, which enhances the solubility of hydrophilic and polar ionic compounds; (2) no need for ion-pairing reagents and high content of organic solvent, which benefits mass spectrometry detection; (3) high orthogonality to reverse-phase liquid chromatography, well adapted to two-dimensional liquid chromatography for complicated samples. Therefore, hydrophilic interaction liquid chromatography has been rapidly developed in many areas over the past decades. This review summarizes the recent progress (from 2012 to July 2016) of hydrophilic interaction liquid chromatography in pharmaceutical analysis, with the focus on detecting chemical drugs in various matrices, charactering active compounds of natural products and assessing biotherapeutics through typical structure unit. Moreover, the retention mechanism and behavior of analytes in hydrophilic interaction liquid chromatography as well as some novel hydrophilic interaction liquid chromatography columns used for pharmaceutical analysis are also described.

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Studies on the retention behavior of quercetin, phenol, and caffeine as test substances on selected neutral and charged hydrophilic interaction liquid chromatography stationary phases

Ziobrowski

Zapała

2022

Separation Science Plus

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The influence of mobile phase composition and column temperature on the retention behavior of quercetin, phenol, and caffeine as test analytes was analyzed. The experiments were conducted in columns filled with the charged amine phase and the neutral amide phase. As mobile phases, the varying fractions of methanol or acetonitrile in water solutions were applied. The most likely retention mechanisms have been discussed. The results of investigations confirm the importance of a mixed adsorption‐partition retention mechanism in the hydrophilic interaction liquid chromatography process, regardless of the mobile phase composition and type of stationary phase. Investigations have also shown that in the tested systems the retention mechanism is also strongly column temperature‐dependent. Additionally, the efficiency of the tested columns was determined and analyzed as a function of the linear flow velocity. The obtained literature known as van Deemter plots confirms generally higher column efficiencies in the systems with acetonitrile as an organic component of the mobile phase. However, in some cases, the application of methanol allows obtaining similar column efficiencies. Furthermore, the results show that both columns tested exhibit comparable efficiency in the most tested systems and provide very good efficiency preservation over the wide range of eluent velocities.

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Studies on the sorption behavior of quercetin, phenol, and caffeine as test substances on diol mixed-mode HILIC column

Cited by 7 publications

References 33 publications

Analysis of adsorption energy distribution in selected hydrophilic‐interaction chromatography systems with amide, amine, and zwitterionic stationary phases

Analysis of adsorption energy distribution in selected hydrophilic‐interaction chromatography systems with amide, amine, and zwitterionic stationary phases

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Studies on the retention behavior of quercetin, phenol, and caffeine as test substances on selected neutral and charged hydrophilic interaction liquid chromatography stationary phases

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