Studies on the Sequence of Uronic Acid Residues in Alginic Acid.

Haug, Arne; Lársen, Bjørn; Smidsrød, Olav; Eriksson, Göran; Blinc, R.; Paušak, S.; Ehrenberg, L.; Dumanović, J.

doi:10.3891/acta.chem.scand.21-0691

Cited by 484 publications

(211 citation statements)

References 0 publications

Supporting

Mentioning

199

Contrasting

Unclassified

Order By: Relevance

“…As shown in the chromatograms in Fig. 4, the hydrolysates gave spots identical with that of authentic sodium alginate, which consists of mannuronic and glucuronic acids [12].…”

Section: Identification Of the Main Component Of Eachmentioning

confidence: 86%

Slime Production by Pseudomonas aeruginosa

Murakawa

1973

Japanese Journal of Microbiology

View full text Add to dashboard Cite

Chemical properties and compositions of slimes produced by two Pseudomonas aeruginosa strains of different colonial types were investigated. The main component of the slime from strain IFO 3445 was found to be DNA, contaminated with small amounts of protein. On the other hand, the slime from a mucoid‐type strain No. 24 was an alginate‐like substance consisting of mannuronic and glucuronic acids, and contained traces of protein and nucleic acid. Slimes from twenty clinical isolates of P. aeruginosa were investigated for their chemical compositions. Slimes from eighteen strains consisted of DNA, while, two strains of a mucoid‐type produced slimes composed of polyuronic acid.

show abstract

“…As shown in the chromatograms in Fig. 4, the hydrolysates gave spots identical with that of authentic sodium alginate, which consists of mannuronic and glucuronic acids [12].…”

Section: Identification Of the Main Component Of Eachmentioning

confidence: 86%

Slime Production by Pseudomonas aeruginosa

Murakawa

1973

Japanese Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Polymannuronate and polyguluronate were prepared from alginate using method of Haug, Larsen & Smidsrød (1967). Briefly, alginate is dispersed in 500 ml 0.3M HCl, and kept in a water bath 100 0 C for 5 hours.…”

Section: Enzyme Activity Asaymentioning

confidence: 99%

“…After centrifuged at 4500 xg for 10 minutes, the supernatant containing M blocks was neutralized, dialyzed and freeze-dried. The insoluble part which is G blocks was also neutralized, dialyzed and freezedried (Haug, Larsen & Smidsrød, 1967).…”

Section: Enzyme Activity Asaymentioning

confidence: 99%

Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate

Subaryono¹,

Ardilasari²,

Peranginangin³

et al. 2017

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

Screening of alginate lyase producing bacteria associated with seaweed Sargassum crassifolium was carried out, and isolate S245, identified as Bacillus megaterium S245 was found to produce high alginate lyase activity. This research was conducted to evaluate activity of the alginate lyase enzyme at various pHs, temperatures and substrates. Polymannuronate and polyguluronate were used to evaluate substrate specificities. Alginate lyase activity was assayed by analysis of reducing sugar released using the 3,5 dinitrosalicylic acid (DNS) method. The research showed that the activity of alginate lyase was optimum at pH of 7.0 and temperature of 45 0 C. This enzyme was active for both polymannuronate and polyguluronate susbtrates. The V max and K m of this enzyme for polymannuronate and polyguluronate were 200 unit/ml/min and 79.8 mg/ml for polymannuronate substrate and 27.78 unit/ml/min and 13.17 mg/ml for polyguluronate substrate. This enzyme showed unique characteristic in working toward the two substrates.

show abstract

“…These uronate units are arranged as a poly(M)-block, a poly(G)-block, and an alternating or random poly(MG)-block. Alginate is found in inter-cellular matrices of brown algae and in biofilms of certain bacteria [1][2][3][4]. Alginate extracted from brown algae is used in a variety of industrial and food materials because of its unique physical properties i.e., the sodium salt solution exhibits high viscosity and the calcium salt forms elastic gel [2,5].…”

Section: Introductionmentioning

confidence: 99%

“…Alginate is an anionic heteropolysaccharide comprised of -D-mannuroate (M) and -L-guluronate (G) [1][2][3]. These uronate units are arranged as a poly(M)-block, a poly(G)-block, and an alternating or random poly(MG)-block.…”

Section: Introductionmentioning

confidence: 99%