Studies on the Role of VP4 of G Serotype 10 Rotavirus (B223) in the Induction of the Heterologous Immune Response in Calves

Xu, Zhichang; Woode, G. N.

doi:10.1006/viro.1993.1478

Cited by 13 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VP4 interacts closely with VP7 on the surface of the mature rotavirus particles, and it has been shown that this interaction may affect the antigenic properties of VP4. Analysis of reassortant viruses having the surface proteins derived from different parental strains has shown that the reactivity of MAbs directed to epitopes located on both VP8 (18) and VP5 (8,41) trypsin cleavage products of VP4 can be enhanced or inhibited, depending on the origin of the companion VP7 protein, and the reactivity of MAbs directed to VP7 epitopes can also be influenced by the origin of VP4 (10). In addition, it has been reported that specific phenotypes, such as plaque size and protease dependence of growth, associated with the outer capsid proteins, can be affected in rotavirus reassortants by the recipient genetic background, and specific interactions between VP4 and VP7 have been proposed as the basis for these phenotypic effects (7).…”

Section: Fig 2 Effect Of Neuraminidase Treatment Of Ma104 Cells On mentioning

confidence: 99%

Interactions between the two surface proteins of rotavirus may alter the receptor-binding specificity of the virus

Méndez

Arias

1996

J Virol

View full text Add to dashboard Cite

The infection of target cells by most animal rotavirus strains requires the presence of sialic acids (SAs) on the cell surface. We recently isolated variants from simian rotavirus RRV whose infectivity is no longer dependent on SAs and showed that the mutant phenotype segregates with the gene coding for VP4, one of the two surface proteins of rotaviruses (the other one being VP7). The nucleotide sequence of the VP4 gene of four independently isolated variants showed three amino acid changes, at positions 37 (Leu to Pro), 187 (Lys to Arg), and 267 (Tyr to Cys), in all mutant VP4 proteins compared with RRV VP4. The characterization of revertant viruses from two independent mutants showed that the arginine residue at position 187 changed back to lysine, indicating that this amino acid is involved in the determination of the mutant phenotype. Surprisingly, sequence analysis of reassortant virus DS1XRRV, which depends on SAs to infect the cell, showed that its VP4 gene is identical to the VP4 gene of the variants. Since the only difference between DS1XRRV and the RRV variants is the parental origin of the VP7 gene (human rotavirus DS1 in the reassortant), these findings suggest that the receptor-binding specificity of rotaviruses, via VP4, may be influenced by the associated VP7 protein.

show abstract

Section: Fig 2 Effect Of Neuraminidase Treatment Of Ma104 Cells On mentioning

confidence: 99%

Interactions between the two surface proteins of rotavirus may alter the receptor-binding specificity of the virus

Méndez

Arias

1996

J Virol

View full text Add to dashboard Cite

show abstract

“…This apparent selection of some genotypes combinations may occur because of the interactions among different variations of the VP7 and VP4 proteins, which modified the strain virulence. Analysis of reassortant rotavirus has shown that the reactivity of monoclonal antibodies towards epitopes, located in VP4 can be inhibited or increased depending on the associated VP7 protein (Chen et al, 1992;Kool et al, 1992;Xu and Woode, 1993). It was also observed that the reactivity of the monoclonal antibodies to the VP7 epitopes can also be influenced by the VP4 origin (Dunn et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

G and P Genotypes of Group A Rotavirus from Diarrhoeic Calves Born to Cows Vaccinated against the NCDV (P[1],G6) Rotavirus Strain

Barreiros

Alfieri

Médici

et al. 2004

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

A total of 71 group A rotavirus field strains, detected by polyacrylamide gel electrophoresis and enzyme immune assay, were submitted to genotyping by a multiplex nested-reverse transcription-polymerase chain reaction (RT-PCR). Faecal samples were obtained from calves with diarrhoea, between January 2000 and December 2001, from 14 dairy and beef cattle herds located in Mato Grosso do Sul, Sa˜o Paulo and Parana´States, Brazil. Thirty-five rotavirus field strains were detected in faeces from calves born to vaccinated dams with inactivated commercial vaccine containing the rotavirus strain P[1],G6 from seven herds while 36 rotavirus field strains were obtained from diarrhoeic calves from other seven herds without rotavirus vaccination. Identification of P and G rotavirus genotypes was possible in 94.3% (67/71) of the strains. However, G genotype could not be identified in two strains, P genotype in another strain and both genotypes were not detected in a third strain. P and G rotavirus genotype associations were identified in rotavirus field strains from non-vaccinated and vaccinated herds at similar rates of 94.4% (34/36) and 94.3% (33/35), respectively. In non-vaccinated herds binary combinations were P[1],G6 (three herds; 12 strains), P[1],G8 (three herds; six strains), P[5],G6 (two herds; 10 strains) and P[11],G8 (one herd; four strains). The genotype P[11],G6 was the most frequent (three herds; 12 strains) in vaccinated herds, followed by P[5],G6 (two herds; three strains), and P[11],G8 (two herds; eight strains). Mixed infections were observed in one non-vaccinated herd (two strains) and in four vaccinated herds (10 strains). Identification of binary combinations was not possible in one strain from nonvaccinated herds and two strains from vaccinated herds. Unusual genotypes were more frequent (91.4%) in nonvaccinated herds than those from prototypes of bovine rotavirus strains. Nebraska calf diarrhoea virus (NCDV)-like genotype was found in none of the rotavirus field strains detected in diarrhoeic calves born of vaccinated dams.

show abstract

“…However, examination of the immune response in infants vaccinated with the bovine RV WC3 vaccine suggested that VP7 is immunodominant (24). A further study in calves infected with the G10 bovine RV B223 strain has suggested that VP7 is the immunodominant neutralizing antigen in the primary response, with VP4 being important in the elicitation of a heterotypic response (27). Results from other animal models suggest that immunodominance may be dependent on the infecting strain (12,26).…”

mentioning

confidence: 99%

Production of reassortant viruses containing human rotavirus VP4 and SA11 VP7 for measuring neutralizing antibody following natural infection

Gorrell

Bishop

1997

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

The outer capsid proteins VP4 and VP7 of group A rotaviruses are both targets of neutralizing antibody produced following natural infection in humans. Of interest is the relative importance and immunodominance of each protein in the generation of a protective immune response. In order to measure neutralizing antibody responses to VP4 and VP7 separately, reassortants bearing VP4 of each of the major human rotavirus P types with VP7 of SA11 origin were successfully produced by neutralizing monoclonal antibody selection. The resulting reassortants, together with reassortants representing each of the major VP7 types, were antigenically characterized with serotype-specific neutralizing monoclonal antibodies and hyperimmune sera. The neutralization proteins of human rotavirus origin were found to be unaffected antigenically by reassortment. The abilities of these reassortants to discriminate between VP4 and VP7 immune responses were evaluated with postinfection sera collected from three patients infected with either a P1A[8],G1, a P1B[4],G2, or a P1A[8], G4 rotavirus strain. The reassortants were shown to be capable of separating the neutralizing antibody responses to VP4 and VP7, with each patient showing a different immune response with respect to VP4 or VP7 immunodominance. These reassortants can now be applied to analyses of individual immune responses to VP4 and VP7 proteins after primary rotavirus infections and reinfections in humans.

show abstract

Studies on the Role of VP4 of G Serotype 10 Rotavirus (B223) in the Induction of the Heterologous Immune Response in Calves

Cited by 13 publications

References 0 publications

Interactions between the two surface proteins of rotavirus may alter the receptor-binding specificity of the virus

Interactions between the two surface proteins of rotavirus may alter the receptor-binding specificity of the virus

G and P Genotypes of Group A Rotavirus from Diarrhoeic Calves Born to Cows Vaccinated against the NCDV (P[1],G6) Rotavirus Strain

Production of reassortant viruses containing human rotavirus VP4 and SA11 VP7 for measuring neutralizing antibody following natural infection

Contact Info

Product

Resources

About