Studies on the Quantitative Autoradiography. I. Radioluminography for Quantitative Autoradiography of 14C.

Motoji, Naomi; Hayama, Emiko; Shigematsu, Akiyo

doi:10.1248/bpb.18.89

Cited by 20 publications

(7 citation statements)

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“…The PCR product including the G2215A site was digested with HaeII at 37°C for 12 h. The digested DNA fragments were separated on an 8% PAG (Fig. 1E), and the dried gel was analyzed by a computerized system for radioluminography (BAS2000, Fuji Photo Film, Kanagawa, Japan; Motoji et al 1995), with analyzing software MacBAS version 2.3 (Fuji Photo Film; Fig. 1F).…”

Section: I/d and G2215a Polymorphismsmentioning

confidence: 99%

Increased amount of the angiotensin-converting enzyme (ACE) mRNA originating from the ACE allele with deletion

et al. 2004

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The insertion/deletion (I/D) polymorphism in intron 16 of the angiotensin-converting enzyme (ACE) gene is involved in the development of cardiovascular diseases. We compared the ACE mRNA expression originating from the allele with a deletion (D allele) and that from the allele with an insertion (I allele) in human white blood cells from ID heterozygotes. We identified the mRNA from the I allele by using the G2215A polymorphism that lies in exon 15 and that was linked to the I/D polymorphism. RNA samples were obtained from 12 healthy heterozygotes of both I/D and G2215A, and every insertion was shown to be linked to 2215G. ACE mRNA was amplified by the reverse transcription/polymerase chain reaction (RT-PCR) method with an end-labeled antisense primer. The PCR products were digested with HaeII and separated by electrophoresis, and the relative radioactivities of the 2215A and 2215G bands were measured on an auto-image analyzer. The results showed that, in every cases, the intensity of the 2215A product (D allele origin) was higher than that of the 2215G product (I allele origin). The mean ratio of 2215A to 2215G was 1.79 (1.11-2.62). Thus, the D allele leads to higher expression of the ACE mRNA and may affect the renin-angiotensin system in local regions.

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Section: I/d and G2215a Polymorphismsmentioning

confidence: 99%

Increased amount of the angiotensin-converting enzyme (ACE) mRNA originating from the ACE allele with deletion

et al. 2004

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“…IP used in this technique was developed by Fuji Photo Film Company in 1988 as a two-dimensional sensor capable of receiving and storing radiant energy [1]. The IP was confirmed to have linear characteristics within a range of 10 1 to 10 5 photostimulated luminescence -background (PSL-BG) units for both 3 H and 14 C [2,3]. Here, 100 PSL/mm 2 is defined to the output from the BAS system obtained when IP is irradiated by X-ray (80 kV, 0.15 mR) with W target.…”

Section: Introductionmentioning

confidence: 99%

Imaging plate characteristics of positron emitters: ¹¹C, ¹³N, ¹⁵O, ¹⁸F and ³⁸K

Noguchi¹,

Suzuki²

2001

Radiochimica Acta

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Imaging Plate / Positron emitter / Spatial resolution / Dynamic range / SensitivitySummary. Imaging Plate (IP) characteristics were evaluated using 5 positron emitters with different β + energies: 11 C, 13 N, 15 O, 18 F and 38 K. The IP sensitivity showed a wide dynamic range (17-170 000 disintegrations/mm 2 ), allowing extremely low radioactivities to be measured. The uniformity of sensitivity on each IP was ±2.63% ∼ ±3.44%. Spatial resolution was dependent on the energy and tended to deteriorate when radionuclides with a higher energy were used; 330 ± 24 µm for 18 F (E max (β + ) 0.63 MeV) and 470 ± 44 µm for 38 K (E max (β + ) 2.68 MeV), respectively. Self-absorption was also found to depend on the thickness of the slice and the β + energy under the experimental conditions.

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“…Subsequently, the dried sections were exposed overnight to an imaging plate (BAS SR2040, 20 × 40 cm 2 ; Fujifilm, Tokyo, Japan). The imaging plate was then scanned with a phosphor image reader (FLA-5100; Fujifilm, Tokyo, Japan), and the obtained images were analysed using Multi Gauge 3.0 software (Fujifilm, Tokyo, Japan) to calculate the striatal specific uptake ratio (SUr), which is defined as (PSL striatum − PSL cerebellum )/PSL cerebellum [41], where PSL is the photostimulated-luminescence intensity [42, 43]. Five consecutive brain sections from each animal were used to determine the target PSL.…”

Section: Methodsmentioning

confidence: 99%

Quantitative analysis of the therapeutic effect of magnolol on MPTP-induced mouse model of Parkinson’s disease using in vivo 18F-9-fluoropropyl-(+)-dihydrotetrabenazine PET imaging

et al. 2017

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18F-9-Fluoropropyl-(+)-dihydrotetrabenazine [18F-FP-(+)-DTBZ] positron emission tomography (PET) has been shown to detect dopaminergic neuron loss associated with Parkinson’s disease (PD) in human and neurotoxin-induced animal models. A polyphenol compound, magnolol, was recently proposed as having a potentially restorative effect in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)- or 6-hydroxydopamine-treated animal models. In this study, 18F-FP-(+)-DTBZ PET was used to determine the therapeutic efficacy of magnolol in an MPTP–PD mouse model that was prepared by giving an intraperitoneally (i.p.) daily dose of 25 mg/kg MPTP to male C57BL/6 mice for 5 consecutive days. Twenty-minute static 18F-FP-(+)-DTBZ PET scans were performed before MPTP treatment and 5 days after the termination of MPTP treatment to set up the baseline control. Half of the MPTP-treated mice then received a daily dose of magnolol (10 mg/kg dissolved in corn oil, i.p.) for 6 days. 18F-FP-(+)-DTBZ PET imaging was performed the day after the final treatment. All 18F-FP-(+)-DTBZ PET images were analysed and the specific uptake ratio (SUr) was calculated. Ex vivo autoradiography (ARG) and corresponding immunohistochemistry (IHC) studies were conducted to confirm the distribution of dopaminergic terminals in the striatum. The striatal SUr ratios of 18F-FP-(+)-DTBZ PET images for the Sham, the MPTP, and the MPTP + Magnolol-treated groups were 1.25 ± 0.05, 0.75 ± 0.06, and 1.00 ± 0.11, respectively (n = 4 for each group). The ex vivo 18F-FP-(+)-DTBZ ARG and IHC results correlated favourably with the PET imaging results. 18F-FP-(+)-DTBZ PET imaging suggested that magnolol post-treatment may reverse the neuronal damage in the MPTP-lesioned PD mice. In vivo imaging of the striatal vesicular monoamine transporter type 2 (VMAT2) distribution using 18F-FP-(+)-DTBZ animal PET is a useful method to evaluate the efficacy of therapeutic drugs i.e., magnolol, for the management of PD.

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Studies on the Quantitative Autoradiography. I. Radioluminography for Quantitative Autoradiography of 14C.

Cited by 20 publications

References 0 publications

Increased amount of the angiotensin-converting enzyme (ACE) mRNA originating from the ACE allele with deletion

Increased amount of the angiotensin-converting enzyme (ACE) mRNA originating from the ACE allele with deletion

Imaging plate characteristics of positron emitters: ¹¹C, ¹³N, ¹⁵O, ¹⁸F and ³⁸K

Quantitative analysis of the therapeutic effect of magnolol on MPTP-induced mouse model of Parkinson’s disease using in vivo 18F-9-fluoropropyl-(+)-dihydrotetrabenazine PET imaging

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Studies on the Quantitative Autoradiography. I. Radioluminography for Quantitative Autoradiography of 14C.

Cited by 20 publications

References 0 publications

Increased amount of the angiotensin-converting enzyme (ACE) mRNA originating from the ACE allele with deletion

Increased amount of the angiotensin-converting enzyme (ACE) mRNA originating from the ACE allele with deletion

Imaging plate characteristics of positron emitters: 11C, 13N, 15O, 18F and 38K

Quantitative analysis of the therapeutic effect of magnolol on MPTP-induced mouse model of Parkinson’s disease using in vivo 18F-9-fluoropropyl-(+)-dihydrotetrabenazine PET imaging

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Imaging plate characteristics of positron emitters: ¹¹C, ¹³N, ¹⁵O, ¹⁸F and ³⁸K