Studies on the proteins of human seminal plasma I. Characterisation of two esterase systems

Tk, Roberts; Ks, Rao; Masson, Pl.; Jf, Heremans

doi:10.1016/0005-2795(72)90224-3

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Double immunodiffusion and immunoelectrophoresis were carried out in 1.5% agarose at pH 8.6. Electrophoresis, immunodiffusion and immunoelectrophoresis plates were stained, after incubation with anaphthyl acetate, by diazodianisidine as previously described (Roberts et al-1972).…”

Section: Methodsmentioning

confidence: 99%

Two Esterases Common to Seminal Plasma, other External Secretions and Leucocytes in Man

Roberts

Masson²,

Lauwerys³

et al. 2009

Andrologia

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Resumen Dos esterasas comunes al plasma seminal, a otras secreciones externa y a los leucocitos, en el hombre Las esterasas previamente identificadas en el plasma seminal, por su movilidad elec‐troforética, inmunogenicidad y sensibilidad a los esteres orgánicos de fosforo, se detectaron en diversas secreciones humanas externas y en extractos de leucocitos. La esterasa se encontró abundantemente en la orina y en la leche, mientras que en el plasma seminal, extracto de leucocitos, moco cervical y sudor la concentracion fué baja. La concentración de betagama‐esterasa fué alta enel plasma seminal y extracto de leucocitos. La enzima estaba presente en pequeña cantidad en la orina, leche, jugo ‐ gástrico, saliva, esputo, lagrimas, moco cervical y sudor. Zusammenfassung Zwei Esterasen im Seminaplasma, in anderen extemen Sekreten und in Leukocyten des Mannes Zwei Esterasen,‐ die vorher im Seminalplasma identifiziert worden waren, wurden auf der Basis ihrer elektrophoretischen Mobilität, ihrer Immuneigenschaften und Sensitivität gegen Organphosphorester in verschiedenen extemen Sekreten und Leukocytenextrakten bestimmt. α‐Esterase wurde weder im Urin noch in der Milch nachgeweisen, während dieses Ferment in niedriger Konzentration im Seminaplasma, im Leukocytenextrakt, im Zervikalmukus und im Schweiß nachzuweisen war. Die Konzentration der βγ‐Esterase war im Seminalplasma und im Leukocytenextrakt hoch; in geringer Konzentration konnte dieses Enzym im Urin, in der Milch, im Magensaft, Sputum, Tränen, Zervikalschleim und Schweiß nachgewiesen werden.

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Section: Methodsmentioning

confidence: 99%

Two Esterases Common to Seminal Plasma, other External Secretions and Leucocytes in Man

Roberts

Masson²,

Lauwerys³

et al. 2009

Andrologia

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“…Esterase activity was localized using the staining procedure of Roberts et al (1972). The combined procedures of Barka (1961) and Beckman et al (1968) were used for localization of acid phosphatase activity.…”

Section: Methodsmentioning

confidence: 99%

Studies on the Organic Components of Shell Gland Fluid and the Hen's Egg Shell

Salevsky

Leach

1980

Poultry Science

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Shell gland fluid was collected from laying hens during the plumping stage of egg shell formation. Disc-gel electrophoresis of this fluid demonstrated the existence of seven to nine components which stained for protein. Chemical analysis of fluid indicated that this material differed considerably from the organic portions of egg shell with respect to carbohydrate and amino acid composition. This suggests that the proteins present in shell gland fluid do not serve as direct precursors of the organic components of egg shell. The proteins present in shell gland fluid were found to exhibit carbonic anhydrase, acid phosphatase, and several types of esterase activity. Alkaline phosphatase activity was present in very low amounts. On the basis of inhibitor studies, uterine fluid acid phosphatase activity was found to differ from blood acid phosphatase and exhibited properties similar to prostatic acid phosphatase. Carbonic anhydrase activity of shell gland fluid did not vary with the stage of egg shell formation nor were there differences in the activity of fluid taken from hens selected for differences in egg shell thickness.

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Esterase activity associated with secretory IgA and free secretory component preparations from human milk

Kobayashi¹,

Roberts²,

Vaerman³

et al. 1973

Biochimica et Biophysica Acta (BBA) - Protein Structure

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Studies on the proteins of human seminal plasma I. Characterisation of two esterase systems

Cited by 4 publications

References 18 publications

Two Esterases Common to Seminal Plasma, other External Secretions and Leucocytes in Man

Two Esterases Common to Seminal Plasma, other External Secretions and Leucocytes in Man

Studies on the Organic Components of Shell Gland Fluid and the Hen's Egg Shell

Esterase activity associated with secretory IgA and free secretory component preparations from human milk

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