Studies on the properties of the human alcohol dehydrogenase isozymes determined by the different loci ADH1, ADH2, ADH3

Smith, Moyra; Hopkinson, D. A.; Harris, Harry

doi:10.1111/j.1469-1809.1973.tb01814.x

Cited by 113 publications

(23 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three of these were represented by strong autoradiography signals at 1.5, 1.9 and 2.8 kb, while signals corresponding to 4.3 and 5.5 kb were faint. The probe used for class I ADH, cross-hybridised with transcripts originating from all three class I loci (ADHI-3), which give rise to the a-, p-and ysubunits [25]. The different sizes of class I transcripts can be explained by transcription from the three different loci and the use of alternative poly-A signals [14,15,26].…”

Section: Resultsmentioning

confidence: 99%

Alcohol dehydrogenase in human tissues: localisation of transcripts coding for five classes of the enzyme

1996

View full text Add to dashboard Cite

Tissue distribution of the five identified classes of human alcohol dehydrogenase was studied by assessment of mRNA levels in 23 adult and four fetal tissues. Alcohol dehydrogenase of class I was found in most tissues, brain and placenta excluded, but expression levels among tissues differed widely. The distribution pattern of class III transcripts was consistent with those of housekeeping enzymes while, in contrast, class IV transcripts were found only in stomach. Transcripts of multiple length were detected for most classes and were due to different gene products arising through the use of different poly-A signals or transcription from different gene loci. Both class II and class V showed a pattern of liver-enriched expression. However, low mRNA levels were detected also in stomach, pancreas and small intestine for class II, and in fetal kidney and small intestine for class V. Significantly higher levels of class V transcripts were present in fetal liver when compared with levels in adult liver, which suggests that human class V is a predominantly fetal alcohol dehydrogenase.

show abstract

Section: Resultsmentioning

confidence: 99%

Alcohol dehydrogenase in human tissues: localisation of transcripts coding for five classes of the enzyme

1996

View full text Add to dashboard Cite

show abstract

“…They belong, respectively, to the alcohol dehydrogenase and aldehyde deydrogenase families, ADH2 being expressed in normal lung (31). Genes belonging to these two families are usually regulated in parallel.…”

Section: Discussionmentioning

confidence: 99%

Lung Cancers Detected by Screening with Spiral Computed Tomography Have a Malignant Phenotype when Analyzed by cDNA Microarray

Bianchi

Pelosi

et al. 2004

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose: Spiral computed tomography (CT) can detect lung cancer at an early stage, but the malignant potential is unknown. The question is, as follows: do these small lesions have the same lethal potential as do symptomatic tumors?Experimental Design: We used a cDNA microarray platform and compared the gene expression profile of spiral CT-detected lung carcinomas with a matched case-control population of patients presenting with symptomatic lung cancer.Results: CT-detected and symptomatic tumors have shown a comparable gene expression profile. Correspondence analysis has demonstrated that nine genes were differentially expressed, although with a high variability across the samples that prevented distinguishing the two groups of tumors. Analysis of these nine genes has suggested that early-detected tumors have higher levels of retinoic acid production and higher expression levels of caveolin 2, matrix Gla, and cystatin A, which are already known to be lost during tumor progression.Conclusions: All of the tumors observed are histologically malignant according to the WHO Classification. Early lung cancers that are detected by screening have a gene expression pattern similar to, but not identical to, that of symptomatic lung carcinomas.

show abstract

“…Peptide analyses have revealed that the peptide prof'des of Class I ADH (homo-or heterodimers of a,/3, and 3, subunits), Class II ADH (dimers of zr subunits), and Class III ADH (dimers of X subunits) differ significantly from each other while ct,/3, and 3' subunits within Class I have very similar profiles [14]. Also, subunits from one class do not form heterodimers with subunits from the other two classes [6,8,10]. Class I ADH isozymes are the products of three gene loci, ADHI, ADH2, and ADH3, which encode the three different polypeptide chains designated c~, /3, and -/, respectively [I0].…”

mentioning

confidence: 74%

“…Isozymes of the three classes differ from each other in terms of electrophoretic mobility, substrate specificities, inhibition characteristics and stability [6,8,10]. Peptide analyses have revealed that the peptide prof'des of Class I ADH (homo-or heterodimers of a,/3, and 3, subunits), Class II ADH (dimers of zr subunits), and Class III ADH (dimers of X subunits) differ significantly from each other while ct,/3, and 3' subunits within Class I have very similar profiles [14].…”

mentioning

confidence: 99%

Molecular genetic analysis of human alcohol dehydrogenase

Duester¹,

Hatfield²,

Smith³

1985

Alcohol

Self Cite

View full text Add to dashboard Cite

Studies on the properties of the human alcohol dehydrogenase isozymes determined by the different loci ADH₁, ADH₂, ADH₃

Cited by 113 publications

References 13 publications

Alcohol dehydrogenase in human tissues: localisation of transcripts coding for five classes of the enzyme

Alcohol dehydrogenase in human tissues: localisation of transcripts coding for five classes of the enzyme

Lung Cancers Detected by Screening with Spiral Computed Tomography Have a Malignant Phenotype when Analyzed by cDNA Microarray

Molecular genetic analysis of human alcohol dehydrogenase

Contact Info

Product

Resources

About