Studies on the postnatal development of the collecting ducts of the rat kidney, with particular reference to their glycogen content

Wake, C.; Moffat, D. B.; Creasey, Margaret; Clague, H. W.

doi:10.1159/000144264

Cited by 5 publications

(2 citation statements)

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“…l), the decline in glycogen concentration must be interpreted as a glywgen dilution by proteins, which completely masks the slight increase in glywgen wntent. In neonatal rat kidney, glywgen wntent is particularly abundant in collecting ducts (2,25). In fetal rat kidney, we controlled the localization of glywgen: PAS-positive material observed in most of the cells of collecting ducts disappeared after amyloglucosidase treatment (data not shown).…”

Section: Methodsmentioning

confidence: 99%

“…Glywgen turnover rate is more rapid in the medulla than in the cortex (18). It has been observed in several species (2, 15,25) that the glycogen concentration in kidneys of newborn animaln is relatively high and as far as we know, no data are available in the fetus. In the rat, Dicker and Shirley (8) have made a correlation between the progressive disappearance of renal glycogen during the 2-3 wk after birth and an initially high rate of anaerobic glycolysis that subsequently decreases to levels found in the adult.…”

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Renal Glycogen Content and Hormonal Control of Enzymes Involved in Renal Glycogen Metabolism

et al. 1983

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Fetal rat kidney showed g l y c y deposition that reached a maximal value of 60 pg *mg prot on day I8 and declined thereafter. At birth glycogen concentration is reduced (20 ~m m g prot-') but higher than adult one (cortex, 2.2 p g a m g prot-a d medulla, 3.4 p g * m g prot-I). From day 17 to the birtb, gtycogen synthetase and phospborylase activities increased slowly except for acid glucosidase activity which inereased rapidly between day 18 to the birth (3-fold). Corticosterokl deprivation had no effect upon glycogen content but fetal decapitation on day 16 reduced glycogen content in kidney of 19day-old fetuses.In the adult kidney, the concentration of glycogen, which is mainly localized in collecting ducts (18,20) is extremely low compared with other organs such as liver, heart of muscle. Glywgen turnover rate is more rapid in the medulla than in the cortex (18). It has been observed in several species (2, 15,25) that the glycogen concentration in kidneys of newborn animaln is relatively high and as far as we know, no data are available in the fetus. In the rat, Dicker and Shirley (8) have made a correlation between the progressive disappearance of renal glycogen during the 2-3 wk after birth and an initially high rate of anaerobic glycolysis that subsequently decreases to levels found in the adult. According to these authors the major source of ATP in neonatal kidney is anaerobic glycolysis whereas aerobic glycolysis predominates in the adult. Consequently, an increased glucose requirement, which may be supplied by glycogen mobilization, may be expected in the kidney of young rats. The differentiation of rat kidney is known to take place a few days before birth (6,21). Assuming that renal glycogen may play an impurtant role in providing energy for difierentiation and maturation during the perinatal period, we studied the evolution of renal glycogen concentration in relationship to enzyme activities involved in glycogen metabolism in fetal and newborn rats. In addition an eventual endocrine control of glycogen metabolism was also investigated. MATERIALS AND METHODSThe female rats used in these experiments were of the Sherman strain whose length of gestation was 21 days. Fetuses between 1 6 21 days of gestation were used. EXPERIMENTAL PROCEDURESMaternal adrenalectomy and metopirone treatment. In order to suppress corticosteroids in fetal plasma, maternal adrenalectomy was associated with metopirone treatment as previously described (10). Maternal adrenalectomy was performed at days 15 or 16 of gestation. Pregnant rats were then injected subcutaneously with metopirone at a dose of 10 mg twice daily until (days 18 or 21). Metopirone, which has been kindly supplied by CIBA laboratory, is a steroid hydroxylase inhibitor crossing the placental barrier. The effectiveness of treatment was routinelv controlled bv verifying the enlargement of the fetal adrenal g&ds. Fetuses i f adrenalectomized-treated mothers were compared with "control" fetuses of sham-omrated mothers.Fetal decapitation. Hypothalamo-hypophyseal and ...

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Renal Glycogen Content and Hormonal Control of Enzymes Involved in Renal Glycogen Metabolism

et al. 1983

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Histochemistry and ultrastructure of the interstitium of the renal papilla in rats with hereditary diabetes insipidus (brattleboro strain)

1980

Am. J. Anat.

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The Brattleboro strain of Long-Evans hooded rats has hereditary hypothalamic diabetes insipidus due to the inability to produce antidiuretic hormone. Animals homozygous for this autosomal recessive trait have extreme polyuria and polydipsia, whereas heterozygotes are less severely affected. Light and electron microscopy were used to study the interstitial tissue of the renal papilla of Brattleboro rats and normal Long-Evans rats. Staining with alcian blue or colloidal iron revealed that homozygous Brattleboro rats (DI) have greatly reduced quantities of glycosaminoglycans in the papillary interstitium. Heterozygotes showed staining similar but not identical to that of normal rats. The papillary interstitial cells of DI rats lacked the cytoplasmic processes seen in normal rats, and the normal relationship of these cells to the tubular elements of the papilla was absent. Electron microscopy revealed that the papillary interstitial cells of DI rats appeared less active than those of heterozygous or normal rats. In DI rats these cells displayed reduced numbers of lipid droplets and mitochondria, and the Golgi apparatus and rough endoplasmic reticulum were poorly developed. The altered ultrastructure of the papillary interstitial cells may be responsible for the reduction of interstitial glycosaminoglycans in DI rats. Glycosaminoglycans possess properties which may contribute to urinary concentration, It is suggested that the interstitial tissue of the renal papilla is influenced by antidiuretic hormone.

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Bargmann¹

1978

Niere Und Ableitende Harnwege

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Studies on the postnatal development of the collecting ducts of the rat kidney, with particular reference to their glycogen content

Cited by 5 publications

References 3 publications

Renal Glycogen Content and Hormonal Control of Enzymes Involved in Renal Glycogen Metabolism

Renal Glycogen Content and Hormonal Control of Enzymes Involved in Renal Glycogen Metabolism

Histochemistry and ultrastructure of the interstitium of the renal papilla in rats with hereditary diabetes insipidus (brattleboro strain)

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