Studies on the Physiology and Biochemistry of Mammalian Epididymis: Biochemical Composition of Epididymis. A Comparative Study

Riar, S. S.; Setty, B.S.; Kar, Amiya B.

doi:10.1016/s0015-0282(16)39673-x

Cited by 52 publications

(6 citation statements)

References 26 publications

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“…However, the exact role of G P C in muscle may remain unexplained, as it is in semen, which contains high levels (Riar et al, 1973). chemical shifts, pK, values of 6.25-6.30 are found for all 5'mononucleotides secondary phosphate ionization, independently from the nature of the base and the presence of a hydroxyl group at the 2' position.…”

Section: Discussionmentioning

confidence: 99%

“…The specific occurrence of GPC in the slow-type muscles would suggest its being involved in the oxidative, energy-producing, metabolic pathways. However, the exact role of GPC in muscle may remain unexplained, as it is in semen, which contains high levels (Riar et al, 1973). A phosphorus-31 nuclear magnetic resonance (NMR) study of adenine, uracil, and thymine mononucleotides, their cyclic analogues, and the corresponding dinucleotides is reported.…”

Section: Discussionmentioning

confidence: 99%

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Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle

Burt¹,

Glonek²,

Bárány³

1976

Biochemistry

160

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In the examination of intact muscles by 31P nuclear magnetic resonance spectroscopy, a number of signals have been detected in the phosphodiester region (-0.5 to 0.5 ppm) of the spectrum which could not be correlated with the known common phosphates of muscle tissue. These signals arise from perchloric acid extractable compounds with several common chemical properties, one of which is a ready solubility in nearly anhydrous ethanol solutions. A component contributing to the major resonance has been identified as glycerol-3-phosphorylcholine. This characterization is based on both 31P nuclear magnetic resonance and chromatographic data.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle

Burt¹,

Glonek²,

Bárány³

1976

Biochemistry

160

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“…It was also reported that androgen promotes the sperm maturation (Orgebin-Crist, 1973). The growth and the secretory state of the epididymis are dependent on androgens (Hamilton, 1972;Rajalakshmi and Prasad, 1971;Riar et al, 1973 In the pressed fluids from the cauda, the total amount of free amino acids was signicase, most of the amino acids was evenly increased. When the content of amino acids was computed into mol %, no significant changes were observed after castration.…”

Section: Fluids Frommentioning

confidence: 99%

Free Amino Acids in the Caput and the Cauda Epididymis of Adult Rats

et al. 1976

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To examine the content and the composition of free amino acids in the intraluminal fluid of rat epididymis, the fluids were obtained by light pressure on the dissected tissues. The amount of the total free amino acids in the pressed fluid from the caput epididymis was significantly higher than those of the cauda epididymis and the testis. Glu and Gln were predominant amino acids in the caput, and their amounts occupied more than half of the total ninhydrin reactive compounds. Such a high concentration of Glu and Gln was not observed either in the cauda or in the testis. Castration decreased Glu and increased Gln in amount. Testosterone treatment to castrated animals did not restore Glu and Gln contents in the pressed fluid from the caput epididymis to the level observed in intact rats completely. Therefore, it was assumed that a large amount of Glu in the caput was due to many factors; secretion and metabolism of epithelial cells of the gland which might be regulated by androgen, inflow of rete testis fluid, and sperm metabolism of amino acids in the epididymis. The results obtained from the caput epididymis to which the efferent duct of the testis was ligated also supported this interpretation.

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“…The sialic acid found at the end of the oligosaccharide chains of many soluble glycoprotein carry a message that determines whether the protein will continue to circulate in the blood stream or be removed by the liver. Sialic acid is concerned with the stabilization of the plasma membrane, maintenance of sperms in a decapitated state, ionic balance in the epididymal plasma and antigen interaction between sperm and epididymal epithelium [52]. The synthesis and/ or secretion of Sialic acid is under androgenic control [53].…”

Section: Discussionmentioning

confidence: 99%

Methomyl induced gonadal dysfunction, biochemical contents and enzyme activities in male albino mice

Bb¹,

Si²

2010

Int J of Biot Appl

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Abstract-Methomyl (S-methyl-1-N [(methyl carbamoyl) oxy] thio acetimidate, is a carbamate broad spectrum insecticide. The Swiss albino mice were administered orally 1, 2, 3, and 4 mg/kg body wt methomyl for 30 days and effective dose 4 mg/kg for 5, 10, and 20 days to know the dose and durational effect on testes and biochemical contents. The mice were sacrificed on day 31 st or 24 hours after the terminal exposure. Testes, epididymis, vasa deferentia, seminal vesicles, prostate, coagulatory and Cowper's glands weight decreased significantly with 2, 3 and 4 mg/kg/day and in 4mg/kg /day for 10 and 20 days of methomyl treatment.Histologic studies of the testes revealed anti-spermatogenic and anti-steroidogenic effects of methomyl reflected by significant decrease in the number and diameter of spermatogonia, primary spematocytes, secondary spermatocytes, spermatids and Leydig cells in the mice treated with 2, 3 and 4 mg/kg/day and in 4 mg/kg /day for 10 and 20 days of methomyl treatment, except with 2 mg/kg/day methomyl treatment number of primary spematocytes, Leydig cells, diameter of spermatogonia, and in 4 mg/kg/day methomyl for 10 days treatment the number of spermatogonia, leydig cells and diameter of primary spermatocytes not changed significantly when compared with controls. Biochemical studies of testis and epididymis showed methomyl at doses of 2, 3 and 4 mg/kg/day and in 4 mg/kg /day for 10 and 20 days of methomyl treatment caused significant decrease in the levels of DNA, RNA, protein, glycogen and sialic acid whereas cholesterol increased significantly. However, treatment with 1, 2 mg / kg/ day and 4 mg / kg /day for 5 days methomyl caused no significant change in epididymal biochemical contents when compared with control. Study on enzyme activities in testis and epididymis revealed that the treatment with 3 and 4 mg/kg/day and 4 mg/kg /day for 20 days of methomyl caused significant decrease in the activities of succinic dehydrogenase (SDH), ions ATPase (Na + -K + ATPase, Ca ++ ATPase, Mg ++ ATPase) and acid phosphatase (ACP) were decreased significantly, except with 3 mg/kg/day methomyl treatment where Ca ++ ATPase activity in testes and epididymis not changed significantly. However, lactate dehydrogenase (LDH) and alkaline phosphatase (AKP) activities were increased significantly. The testis 3β hydroxysteroid dehydrogenase (3βHSD) decreased significantly with 3 and 4 mg/kg /day and in 4 mg/kg /day for 10 and 20 days of methomyl treatment when compared with controls. However, there was no significant change in testes and accessory reproductive organs, number and diameter of spermatogonia and Leydig cells and biochemical contents of testis and epididymis with 1 mg/kg/day and in 4 mg/kg /day for 5 days of methomyl treatment. These observed effects of methomyl on testes and accessory reproductive organs weight, testis histology, biochemical contents and enzyme activities in testis and epididymis may be due to toxic effect or hormonal imbalance in any of the stage in the hypothalamo-hypophysial-testicular ...

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Studies on the Physiology and Biochemistry of Mammalian Epididymis: Biochemical Composition of Epididymis. A Comparative Study

Cited by 52 publications

References 26 publications

Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle

Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle

Free Amino Acids in the Caput and the Cauda Epididymis of Adult Rats

Methomyl induced gonadal dysfunction, biochemical contents and enzyme activities in male albino mice

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