“…Significantly higher ( p < 0.05) extraction yield was found with ROFW [25.88%] than the UFW [6.07%]. Extraction yield was observed to be increased after SSF, which was mainly due to the fact that after colonization of fungus, wheat was degraded and more water soluble substances like phenolics, sugars, organic acids and pigments were released [3]. Same concentration (10 mg/ml) of each freeze-dried extract was prepared in water and TPC was compared.…”
HighlightsSSF was done for the enhanced production and extraction of phenolics antioxidants from wheat.A newly isolated Rhizopus oryzae RCK2012 was used in SSF.Different conditions were optimized for the extraction of phenolics from fermented wheat.Compositional analysis of released phenolics was carried out by UPLC and TLC.
“…Significantly higher ( p < 0.05) extraction yield was found with ROFW [25.88%] than the UFW [6.07%]. Extraction yield was observed to be increased after SSF, which was mainly due to the fact that after colonization of fungus, wheat was degraded and more water soluble substances like phenolics, sugars, organic acids and pigments were released [3]. Same concentration (10 mg/ml) of each freeze-dried extract was prepared in water and TPC was compared.…”
HighlightsSSF was done for the enhanced production and extraction of phenolics antioxidants from wheat.A newly isolated Rhizopus oryzae RCK2012 was used in SSF.Different conditions were optimized for the extraction of phenolics from fermented wheat.Compositional analysis of released phenolics was carried out by UPLC and TLC.
“…Several hydrolysing enzymes produced during SSF process are predicted to be associated with the release of PCs (Bhanja et al . , ; Robledo et al . ; Cho et al .…”
Section: Resultsmentioning
confidence: 99%
“…Samples with same concentrations of TPC may vary remarkably in their antioxidant property (Bhanja et al . ; Đorđević et al . ).…”
Antioxidant-rich food products are getting popularity day by day. In this study, potential of solid-state fermentation (SSF) has been studied for the improvement of antioxidant potential of different cereals by GRAS micro-organisms. The comparative evaluation of the antioxidant potential of various fungal fermented products derived from whole grain cereals, such as wheat, brown rice, oat and maize, has been carried out. Among these, Rhizopus oryzae RCK2012-fermented wheat was observed as a potent source of natural antioxidants. A diet containing fermented cereals would be useful for the prevention of free radical-mediated diseases.
“…Hence, it is established that the enzymatic hydrolysis improves the nutraceutical potential of apple juice by releasing the free aglycones. Again, it is proved that not only the phenolic content, but also phenolic composition or structure plays a major role for antioxidant activity (Bhanja et al , 2008). …”
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T660nm = 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl2. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property.
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