Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus: The reduced Na+ level is the basis of the increased salt tolerance

Sun, Yanfang; Wang, Dan; Bai, Yanling; Wang, Ningning; Wang, Yong

doi:10.1007/s11434-006-1306-y

Cited by 34 publications

(25 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different subcellular compartmentalization mechanisms have been described in the plant response to saline stress (revised by Munns and Tester, 2008; Teakle and Tyerman, 2010), including the participation of ion transporters such as CLC and NHX1 , for Cl − and Na + respectively (Bao et al, 2014; Jossier et al, 2010; Nakamura et al, 2006). In this sense, expression of CLC was strongly correlated with salinity tolerance in grapevine (Henderson et al, 2014), while overexpression of NHX1 was demonstrated to enhance salt stress in Arabidopsis and even L. corniculatus (Liu et al, 2010; Sun et al, 2006). These transgenic plants showed a higher Na + accumulation in their tissues, but a higher photosynthetic capacity.…”

Section: Discussionmentioning

confidence: 99%

Contrasting response of twoLotus corniculatusL. accessions to combined waterlogging-saline stress

Antonelli

Calzadilla

Campestre

et al. 2020

Preprint

View full text Add to dashboard Cite

Waterlogging and salinity impair crops growth and productivity worldwide, being their combined effects larger than the additive effects of both stresses separately. Recently, a new Lotus corniculatus accession has been collected from a coastal area with a high frequency of waterlogging-saline stress events. This population is diploid and has potential to increase nutritional values of Lotus cultivars used as forages. Due to its environmental niche, we hypothesize that this accession would show a better adaptation to combined waterlogging-saline stress compared to another commonly used tetraploid L. corniculatus (cv. San Gabriel). Shoot and root growth under waterlogging, salinity and combined waterlogging-saline treatments were addressed, together with chlorophyll fluorescence and gas exchange measurements. Results showed that salinity and waterlogging effects were more severe for the tetraploid accession, being the differences larger under the combined stress condition. In addition, Na+, Cl− and K+ concentrations were measured in young and old leaves, and in roots. A larger accumulation of Na+ and Cl− was observed under both saline and combined stress treatments for the tetraploid L. corniculatus, for which ion toxicity effects were evident. The expression of the NHX1 and CLC genes, coding for Na+ and Cl− transporters respectively, was only increased in response to combined stress in the diploid L. corniculatus plants, suggesting that ion compartmentalization mechanisms were induced in this accession. As a conclusion, the recent characterized L. corniculatus might be used for the introduction of new tolerant traits to combined stresses, in other Lotus species used as forage.

show abstract

Section: Discussionmentioning

confidence: 99%

Contrasting response of twoLotus corniculatusL. accessions to combined waterlogging-saline stress

Antonelli

Calzadilla

Campestre

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…He et al, 2014;Li et al, 2014;Luo et al, 2005;Pardo et al, 2006; Y. X. Sun et al, 2006;Zhou et al, 2009). Eight candidate genes were chosen, and their expression levels were determined under salt stress conditions.…”

Section: Effects Of Nacl Stress On the Gene Expression Of Key Compomentioning

confidence: 99%

Physiological and transcriptional responses to salt stress in salt‐tolerant and salt‐sensitive soybean (Glycine max [L.] Merr.) seedlings

et al. 2018

View full text Add to dashboard Cite

Excess salinity is a major environmental threat to agriculture. However, the adaptive mechanisms underlying salt tolerance in soybean (Glycine max [L.] Merr.) remain unclear. The aim of this study was to investigate the differences between salt‐tolerant and salt‐sensitive soybeans in their physiological characteristics and the expression patterns of 8 cation antiporters under salt stress. The inhibitory effects of salt stress on growth and photosynthesis in salt‐sensitive soybean were stronger than those in salt‐tolerant soybean. The concentration of phosphate and most other ions in the seedlings significantly declined under a 150‐mM‐NaCl treatment. Relative K+, Mg2+, and P concentrations were significantly positively correlated with relative net photosynthetic rate. GmNHX1 (Glyma.10G158700) and GmNcl1 (Glyma.03g171500) had similar expression patterns in the salt‐tolerant and salt‐sensitive cultivars. Furthermore, a significant correlation was present between the transcript levels of these 2 genes and the Na+ concentration in soybean leaves. In addition to GmSALT3, which is the major gene in soybean for salt tolerance in previous studies, GmNHX1 and GmNcl1 may be associated with salt tolerance in soybean to some extent. The salt‐tolerant cultivars in this study maintained higher K+, Mg2+, and P concentrations in the leaf and had higher photosynthetic rates than the salt‐sensitive cultivar did under salt stress. These findings can provide theoretical references for the molecular breeding of salt‐tolerant soybean and the sustainable use of saline soils.

show abstract

“…Plant Na + ‐H + exchangers (NHXs) are tonoplast localized Na + /H + antiporters that function in sequestering of excessive cytosolic Na + into the vacuole for ameliorating its toxic effects to the metabolic activities in cytosol (Apse et al 1999; Zhang and Blumwald 2001; Zhang et al 2001; Apse and Blumwald 2007). The cDNA clones of two NHX homologs ( GmNHX1 and GmNHX2 ) were isolated from soybean (Li et al 2006b; Sun et al 2006). The tonoplast localization of GmNHX1 was verified by tracking the fluorescent signals of GmNHX1‐YFP fusion protein in transgenic tobacco BY‐2 cells (Li et al 2006b).…”

Section: Mechanisms Of Salt Tolerance In Soybeanmentioning

confidence: 99%

Salt Tolerance in Soybean

Phang

Shao

Lam

2008

JIPB

227

158

View full text Add to dashboard Cite

Soybean is an important cash crop and its productivity is significantly hampered by salt stress. High salt imposes negative impacts on growth, nodulation, agronomy traits, seed quality and quantity, and thus reduces the yield of soybean. To cope with salt stress, soybean has developed several tolerance mechanisms, including: (i) maintenance of ion homeostasis; (ii) adjustment in response to osmotic stress; (iii) restoration of osmotic balance; and (iv) other metabolic and structural adaptations. The regulatory network for abiotic stress responses in higher plants has been studied extensively in model plants such as Arabidopsis thaliana. Some homologous components involved in salt stress responses have been identified in soybean. In this review, we tried to integrate the relevant works on soybean and proposes a working model to describe its salt stress responses at the molecular level.Key words: abiotic stress; Glycine max; ion homeostasis; osmotic adjustment; reactive oxygen species scavenging; salt tolerance; soybean. HM (2008). Salt tolerance in soybean. J. Integr. Plant Biol. 50(10), 1196-1212. Available online at www.jipb.net Due to its high protein and oil content, soybean is an important cash crop for human diet, animal feeds, and biodiesel. It is also used as raw material for many human health and industrial products. Therefore, the demand for soybean is ever increasing worldwide. Phang TH, Shao G, LamSalt stress is one of the abiotic stresses that significantly reduce the yield of soybean. Soybean germplasms display a spectrum of salt tolerance capability. A vast collection of soybean genotypes was screened in order to identify genetic materials showing high tolerance to salt stress (Shao et al. 1986). Currently, breeding is the major strategy to improve the salt tolerance of soybean. However, the efficiency of traditional breeding is low because of the long breeding cycle, incompatible fertilization, and close linkage between abiotic stress tolerance loci and undesirable characters (e.g. low yield) (Wang et al. 2003). Received 18 Mar. 2008 Accepted 18 Jun. 2008 Supported by the Hong Kong RGC Earmarked Grant CUHK4434/04M and the Hong Kong UGC AoE Plant and Agricultural Biotechnology Project AoE-B-07/09 (to H.M. Lam).* Author for correspondence. Molecular breeders suggest a concept of "breeding by design" as a targeted, efficient, and comprehensive strategy to elevate the salt tolerance of soybean (Peleman and Dvan der Voort 2003;Wan 2006). A better understanding of salt stress response and the underlying tolerance mechanism is important for implementation of such a tactic.This review aims to summarize the current understanding on the salt stress responses and tolerance mechanisms in soybean. Effects of Salt Stress on Growth, Agronomy Traits and Seed Quality of SoybeanSoybean is classified as a moderately salt-tolerant crop and the final yield of soybean will be reduced when soil salinity exceeds 5 dS/m (Ashraf 1994). It was shown that the average production of 20 soybean cultivars under nonsal...

show abstract

Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus: The reduced Na+ level is the basis of the increased salt tolerance

Cited by 34 publications

References 37 publications

Contrasting response of twoLotus corniculatusL. accessions to combined waterlogging-saline stress

Contrasting response of twoLotus corniculatusL. accessions to combined waterlogging-saline stress

Physiological and transcriptional responses to salt stress in salt‐tolerant and salt‐sensitive soybean (Glycine max [L.] Merr.) seedlings

Salt Tolerance in Soybean

Contact Info

Product

Resources

About