Studies on the Nutrition of Pasteurella Tularensis

Traub, A.; Mager, J.; Grossowicz, N.

doi:10.1128/jb.70.1.60-69.1955

Cited by 43 publications

(27 citation statements)

References 15 publications

(11 reference statements)

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“…The amino acid utilizarion of P. tularensis LVS is similar to that of "the virulent strains, inasmuch as deamination causes pronounced ammani-I fication of the medium during growth. 6 If the pH is uncontrolled, the culture fluid turns alkaline, and the growth rate of the organism decreases. The most likely route of amino acid metabolism is oxidative deamination.…”

Section: Discussionmentioning

confidence: 99%

Growth and Metabolism of Pasteurella Tularensis LVS

Scharer¹,

Klein²,

Lincoln³

1968

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Section: Discussionmentioning

confidence: 99%

Growth and Metabolism of Pasteurella Tularensis LVS

Scharer¹,

Klein²,

Lincoln³

1968

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“…An interaction between glucose and amino acid requirements with respect to growth was shown by Traub et al (16). The glucose requirement was substantially reduced by increasing the total amino acid content of the medium.…”

mentioning

confidence: 84%

Growth and Metabolism of Live Vaccine Strain of Pasteurella tularensis

Scharer¹,

Klein²,

Lincoln³

1968

Applied Microbiology

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The growth and metabolism of the live vaccine strain of Pasteurella tularensis in different media were investigated. Maximal growth was observed in a medium containing a sulfuric acid digest of casein as amino acid source. Amino acid metabolism produced considerable ammonia, and the rate of ammonia evolution was directly proportional to the growth rate. The most likely route for amino acid breakdown is nonspecific oxidative deamination. Most studies on nutritional requirements of Pasteurella tularensis have been restricted to the virulent strains. Early research on the virulent strain was conducted in peptone-blood medium with cysteine enrichment (7). In blood-free medium, the organism required a generous supply of other amino acids in addition to cysteine. This requirement was satisfied by the incorporation of hydrolysates of gelatin or casein acid digest (9, 14; R. C. Mills et al., Bacteriol. Proc., p. 37, 1949) into the medium. Systematic study of amino acid requirement started with the development of chemically defined media. The first such medium was reported by J. T. Tamura and D. E. Fleming (Bacteriol.

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“…Growth. In preliminary tests with "basal medium B" (Traub et al, 1955), poor or erratic growth of the vaccine strain was obtained. It was later established that growth could be obtained consistently, provided the pH of the medium was reduced from 6.8 to 6.2-6.4.…”

Section: Resultsmentioning

confidence: 99%

“…DISCUSSION Although the chemically defined medium produces a lower viable population, it is otherwise comparable to the complex undefined media used in the production of live tularemia vaccine. The components are essentially the same, except for optimal concentration, as those previously described by Traub et al (1955). The concentration of individual ingredients may be varied within rather wide limits without any apparent effect upon frequency of dissociation.…”

mentioning

confidence: 92%

“…In the past few years, certain chemically defined media (Mager, Traub, and Grossowicz, 1954;Traub, Mager, and Grossowicz, 1955;Nagle, Anderson, and Gary, 1960) have been successfully employed for the growth of various strains of Pasteurella tularensis. Use of a medium of this type facilitates controlled study of the factors affecting growth, dissociation, virulence, and immunogenicity.…”

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confidence: 99%

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Evaluation of Live Tularemia Vaccine Prepared in a Chemically Defined Medium

Chamberlain¹

1965

Applied Microbiology

192

113

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CHAMBERLAIN, ROBERT E. (The National Drug Co., Swiftwater, Pa.). Evaluation of live tularemia vaccine prepared in a chemically defined medium. Appl. Microbiol. 13:232-235. 1965.-A chemically defined medium was prepared which adequately supported growth of a vaccine strain of Pasteurella tularensis. This medium differed from those previously described in: (i) concentration of components, (ii) a requirement for calcium pantothenate to obtain increased growth, and (iii) a low initial pH. Varying the concentration of individual components up to 10 times the standard amount did not increase the viable population or affect dissociation. The vaccine strain grown in this chemically defined medium, although lower in viable population, appears to retain its identity and to be equal in potency to that prepared by the conventional method. This preliminary study indicates the potential utility of this medium as a basis for controlled studies of a live bacterial vaccine in terms of growth characteristics, dissociation, virulence, and immunogenicity.

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Studies on the Nutrition of Pasteurella Tularensis

Cited by 43 publications

References 15 publications

Growth and Metabolism of Pasteurella Tularensis LVS

Growth and Metabolism of Pasteurella Tularensis LVS

Growth and Metabolism of Live Vaccine Strain of Pasteurella tularensis

Evaluation of Live Tularemia Vaccine Prepared in a Chemically Defined Medium

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