Studies on the Nature of the Chloroplast Lamella. I. Preparation and Some Properties of Two Chlorophyll-Protein Complexes^*

Chlorophyll-proteins of thylakoids from wild-type and mutants of barley (Hordeum vulgare L.)

“…Chlorophyll-protein complexes: Originally the chlorophyll-containing bands separated by gel electrophoresis were called chlorophyll-protein complexes (33,34), indicating that chlorophyll and a protein formed a complex. For some time it was thought that such bands on polyacrylamide gels were identical to chlorophyll-protein complexes isolated by detergent treatment of thylakoids followed by purification on sucrose gradients or by column chromatography.…”

“…The procedures used until recently were capable of resolving only three chlorophyll-containing bands (8,27,34) designated, in order of increasing electrophoretic mobility, chlorophyllprotein complex I (CPI), chlorophyll-protein complex II (CPII) and free chlorophyll (34). These chlorophyll proteins have been isolated from many species, and are sensitive to denaturation by heat (11), unfavourable pH (22) and strongly reducing conditions (MAcHOLO, unpublished results).…”

Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein

“…The LHC was discovered when the photosynthetic membranes (thylakoids) of green plants were subjected to SDS/polyacrylamide-gel electrophoresis without thermal denaturation (Ogawa et al, 1966;Thornber et al, 1967). The LHC was one of two chlorophyll-protein (CP) complexes visible in unstained gels.…”

Immunological evidences for a localization of system I on the outside face and of system II on the inside face of the chloroplast lamella

“…This membrane could be made of 2 bound layers, an outside one built of units of only one of the 2 photochemical systems which shields the inside layer, made only of units of the other photosystem; or the lamella could be a single membrane built of a checker board assembly of System I and System II units. The 2 layers model was a convenient picture suggested to explain why System I-enriched subchloroplast particles are first or preferentially removed from the chloroplast lamella by a low concentration of detergents [1][2][3]. But a larger affinity of the detergent to System I than to System II could also explain these results.…”