Studies on the mode of action of glyceollin in soybean incompatibility to the root knot nematode, Meloidogyne incognita

Kaplan, D. T.; Keen, N. T.; Thomason, I. J.

doi:10.1016/s0048-4059(80)80003-8

Cited by 68 publications

(27 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies on the mechanism of action of structurally diverse phytoalexins have implicated the membrane as the site of antimicrobial action (19,23,29,30,34,42). The research detailed in this paper was undertaken to determine the locus of action of glycinol.…”

Section: Introductionmentioning

confidence: 99%

“…Capsidiol (50 micrograms per milliliter) inhibits -lactate-dependent transport of I14CIglycine but not vectorial phosphorylation-mediated transport of 114CIa-methyl glucoside. Thus, capsidiors mechanism of antimicrobial action seems to differ from that of the other phytoalexins examined.Phytoalexins are low-mol-wt antibiotics produced by plants in response to various biotic and abiotic elicitors (1, 2,20,27,31,39 glycinol may inhibit the growth of widely diverse microorganisms by a similar mechanism of action (45).Previous studies on the mechanism of action of structurally diverse phytoalexins have implicated the membrane as the site of antimicrobial action (19,23,29,30,34,42). The research detailed in this paper was undertaken to determine the locus of action of glycinol.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Host-Pathogen Interactions

Weinstein

Albersheim²

1983

Plant Physiol.

123

View full text Add to dashboard Cite

The biochemical basis for the ability of the pterocarpan phytoalexin glycinol (3,6a,9-trihydroxypterocarpan) to inhibit the growth of bacteria was examined. Glycinol These data imply that the antimicrobial activity of glycinol, glyceollin, and coumestrol are due to a general interaction with the bacterial membrane. Capsidiol (50 micrograms per milliliter) inhibits -lactate-dependent transport of I14CIglycine but not vectorial phosphorylation-mediated transport of 114CIa-methyl glucoside. Thus, capsidiors mechanism of antimicrobial action seems to differ from that of the other phytoalexins examined.Phytoalexins are low-mol-wt antibiotics produced by plants in response to various biotic and abiotic elicitors (1, 2,20,27,31,39 glycinol may inhibit the growth of widely diverse microorganisms by a similar mechanism of action (45).Previous studies on the mechanism of action of structurally diverse phytoalexins have implicated the membrane as the site of antimicrobial action (19,23,29,30,34,42). The research detailed in this paper was undertaken to determine the locus of action of glycinol. A comparison ofglycinol's effect on bacterial membranes with the effect on the membranes of other phytoalexins has allowed us to examine the question of whether the mode of action of glycinol is representative of other phytoalexins. (i-z-y+a ), a gift of Dr. R. Kaback, Roche Institute of Molecular Biology, Nutley, NJ, was maintained on nutrient agar (Difco) slants and plates. The E. coli used in the preparation of membrane vesicles was grown on Escherichia medium 63 (9). MATERIALS AND METHODS Materials. L-General. Liquid culture assays of the ability of glycinol to inhibit bacterial growth were performed as described (45). Glycinol concentrations were determined by A at 287 nm in absolute ethanol (Es7 = 5800). Membrane vesicles were prepared from E.coli following the method of Kaback (14), except that a 20-cc syringe equipped with an 18-gauge needle, rather than a Teflon and glass homogenizer, was used for resuspension of the membrane pellet (H. R. Kaback, personal communication). The membrane-vesicle preparations were shown to be free of bacterial contamination by incubation on nutrient agar. Protein concentrations were determined by the procedure of Bradford (7)

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Host-Pathogen Interactions

Weinstein

Albersheim²

1983

Plant Physiol.

123

View full text Add to dashboard Cite

show abstract

“…Glyceollin inhibits fungal and nematode growth in vitro at concentrations similar to localized concentrations found in vivo (6,26).…”

mentioning

confidence: 57%

“…Inhibition of nematode respiration and electron transport from several substrates to 02 in isolated soybean mitochondria by glyceollin has been reported by Kaplan, Keen, and Thomason (6), but the specific site ofinhibition by glyceollin was not determined. We thus began our study with the a priori assumption that glyceollin could play a primary role in plant-affected pathogen toxicity, and autophytotoxicity through effects on mitochondrial electron transport.…”

mentioning

confidence: 85%

Glyceollin: A Site-Specific Inhibitor of Electron Transport in Isolated Soybean Mitochondria

Boydston

Paxton²,

Koeppe³

1983

Plant Physiol.

View full text Add to dashboard Cite

The glyceollin inhibition of electron transport by isolated soybean and corn mitochondria was similar to that of rotenone, acting at site I between the internal NADH Glyceollin, a phytoalexin produced during the hypersensitive response in soybeans, is hypothesized to be responsible for the expression of cultivar-specific resistance of soybeans to Phytophthora megasperma var sojae (7,26), and its accumulation has been correlated with the incompatible response of soybeans to Meloidogyne incognita (5), and Pseudomonos glycinea (8). Glyceollin inhibits fungal and nematode growth in vitro at concentrations similar to localized concentrations found in vivo (6,26).Inhibition of nematode respiration and electron transport from several substrates to 02 in isolated soybean mitochondria by glyceollin has been reported by Kaplan, Keen, and Thomason (6), but the specific site ofinhibition by glyceollin was not determined. We thus began our study with the a priori assumption that glyceollin could play a primary role in plant-affected pathogen toxicity, and autophytotoxicity through effects on mitochondrial electron transport. The experiments reported here deal only with the autophytotoxicity of glyceollin on electron transport of mitochondria isolated from etiolated soybean hypocotyls and cotyledons and corn shoots. All studies were designed to determine the unique site of glyceollin effect on electron transport and utilized various substrates, electron acceptors, and known inhibitors. MATERIALS AND METHODSSoybean seedlings (Glycine max L., cv Amsoy 71) were germinated and grown in the dark at 28°C in moist vermiculite saturated Isolation of mitochondria from cotyledon tissue was the same, except that the cotyledons were ground through an Oster Automatic Juicer (model 362; Oster Co., Milwaukee, WI) and filtered through Miracloth (Chicopee Mills, Inc., Milltown, NJ) before the first centrifugation.Purification of washed mitochondria consisted of resuspending washed mitochondria in 1 ml of standard resuspension medium consisting of 0.3 M mannitoL 20 mm Hepes, 0.1 mm EDTA, and 0.1% BSA at pH 7.2. The resuspended mitochondria were then placed on top of a discontinuous sucrose gradient similar to that used by Douce et aL (1), and consisting of 1.45 M sucrose (4 ml) layered under 1.2 M sucrose (10 ml). Tubes were centrifuged for 45 min at 60,000g at 4°C in a Sorvall AH 627 swinging bucket rotor placed in a Sorvall OTD 65 centrifuge.After centrifugation, mitochondria were located at the boundary of the two sucrose layers and were removed with a curved tip pasteur pipette. The purified mitochondria were then diluted slowly over a period of 20 min with the slow addition of 0.1 M sucrose containing 10 mm KH2PO4, and 0.1% BSA until a sucrose concentration of 0.3 M (pH 7.2) was achieved. The diluted mitochondrial suspension in 0.3 M sucrose was then centrifuged at 14,600g for 8 min. After centrifugation, the purified mitochondria were resuspended in the standard 0.3 M mannitol resuspension medium. Mitochondrial protein was esti...

show abstract

“…Différents composés supprimant la reconnaissance, et donc la réaction potentielle de l'hôte, sont décrits par divers auteurs (Doke, 1975;Garas et al, 1979;Doke et Tomiyama, 1980;Oku et al, 1980;Heath, 1982 (Gnanamanickam et Patil, 1977;Tietjen et Matern, 1984;Rouxel et al, 1988;Rouxel, 1988 Smith, 1979;Kaplan et al, 1980;Rossall et al, 1980;Van Etten et al, 1982;Smith et aL, 1984;Lucy et al, 1988 Bailey, 1982aBailey, , 1983Ward, 1986 (Dahiya et Rimmer, 1988) ou du couple piment/Phytophthora capsici (Molot et al, 1985). Dans le premier cas, une accumulation intense de méthoxybrassinine et de cyclobrassinine accompagne une «réaction hypersensible inefficace» de la plante vis-à-vis du champignon (Dahiya et Rimmer, 1988;Rouxel, 1988 Il faut enfin insister sur le fait que l'accumulation intense de phytoalexines n'est que l'une des réponses associées à la réaction hypersensible (Bell, 1980(Bell, , 1981Goodman et al, 1986;Heath, 1986 (Bailey, 1982a …”

Section: Déterminisme Génétiqueunclassified

Les phytoalexines et leur intervention dans la résistance hypersensible aux champignons phytopathogènes

Rouxel¹

1989

Agronomie

View full text Add to dashboard Cite

Studies on the mode of action of glyceollin in soybean incompatibility to the root knot nematode, Meloidogyne incognita

Cited by 68 publications

References 12 publications

Host-Pathogen Interactions

Host-Pathogen Interactions

Glyceollin: A Site-Specific Inhibitor of Electron Transport in Isolated Soybean Mitochondria

Les phytoalexines et leur intervention dans la résistance hypersensible aux champignons phytopathogènes

Contact Info

Product

Resources

About