Studies on the Methylation of Avian Sarcoma Proviruses in Permissive and Non-permissive Cells

Katz, Richard A.; Mitsialis, S. Alex; Guntaka, Ramareddy V.

doi:10.1099/0022-1317-64-2-429

Cited by 20 publications

(9 citation statements)

References 20 publications

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“…The source of the RSV env gene was pATV-8, a recombinant pBR322 plasmid containing the entire genome of the Prague C strain [28]. Wild-type SV40 DNA and DNA from a viable early deletion mutant, d11055 [29,30], were also used.…”

Section: Cells and Virusesmentioning

confidence: 99%

Alterations in the transport and processing of Rous sarcoma virus envelope glycoproteins mutated in the signal and anchor regions

Wills

Hardwick

Shaw

et al. 1983

J of Cellular Biochemistry

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The env gene of Rous sarcoma virus codes for two glycoproteins which are located on the surface of infectious virions. Subcloning of these coding sequences in the place of the late region of SV40 DNA has allowed the expression of a normally glycosylated, functionally active glycoprotein complex on the surface of monkey cells. Through the use of site-directed mutagenesis, the role of specific amino acids in the signal peptide, signal peptidase cleavage site, and membrane anchor region have been investigated. Amino-terminal mutations have shown that deletion of the signal peptidase cleavage site along with one or two amino acids of the hydrophobic signal peptide results in the synthesis of an unglycosylated, uncleaved, and presumably cytoplasmically located precursor. Nevertheless, changing the signal peptidase cleavage site from ala/asp to ala/asn does not block the translocation of the glycoprotein across the membrane or the action of the peptidase. At the other end of the molecule, carboxy-terminal mutations have shown that the deletion of the hydrophobic membrane anchor region is not sufficient for the secretion of the truncated glycoprotein. Interpretations of these results based on recent models for protein transport and secretion are discussed.

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Section: Cells and Virusesmentioning

confidence: 99%

Alterations in the transport and processing of Rous sarcoma virus envelope glycoproteins mutated in the signal and anchor regions

Wills

Hardwick

Shaw

et al. 1983

J of Cellular Biochemistry

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“…Nevertheless, we propose that after elimination of posthatching viraemia, all D14 and D16 ducks go through a phase in which virus expression is in some way blocked. Such lack of expression might be a consequence of infection of resting cells (Fritsch & Temin, 1977), the influence of cis-acting regulatory signals (Cooper & Temin, 1976;Akroyd et al, 1987), or methylation (Katz et al, 1983;Dyson et al, 1985). Decreased expression of viral products should allow the infected cells to escape the immune surveillance directed against cells antigenically modified by ALV proteins.…”

Section: Discussionmentioning

confidence: 99%

Ducks: a new experimental host system for studying persistent infection with avian leukaemia retroviruses

Nehyba¹,

Svoboda²,

Karakoz³

et al. 1990

Journal of General Virology

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“…Total RNA was isolated from cultured AKN-1 ceils, cultured hmnan hepatocytes, freshly isolated human hepatocytes, and human and rat livers, and subjected to Northern blot analysis as described (2,22,23). Cloned cDNAs used for hybridization included those encoding for human albumin (2.2 kb mRNA; kindly provided by Dr. Joseph Locker, University of Pittsburgh, Pittsburgh, PA), rat argininosuccinate synthetase (1.67 kb mRNA) (20), rat tyrosine aminotransferase (2.4 kb mRNA) (7), and rat 18S ribosomal RNA (1.87 RNA) (13).…”

Section: Fluorescence-activated Cell Sorting ( Fa Cs ) Analysis Facsmentioning

confidence: 99%

Isolation and characterization of a human hepatic epithelial-like cell line (AKN-1) from a normal liver

Nüssler

Vergani

Gollin

et al. 1999

In Vitro Cell.Dev.Biol.-Animal

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The isolation and characterization of human liver cell lines are rather difficult due to limited material and poor growth in cell culture. In this report, we present the isolation, culture and characterization of a new epithelial-like liver cell line (AKN-1) with a heterogeneous cell population and many characteristics of the biliary epithelium. The AKN-1 cell line stained positively with antibodies to epithelial cytokeratin polypetides CK 8, 18, and 19. In addition, the cell line expressed the anti-human epithelial-related antigen (MOC-31), the human epithelial antigen (HEA), and the gamma-glutamyl transpeptidase, the hematopoietic growth factor, stem cell factor, and also its receptor, c-kit. The cell line failed to express albumin and factor 8 by immunohistochemistry. It did show, however, a twofold increase in 7-ethoxyresorufin-O-deethylase activity. Cytogenetic characterization revealed rare breakpoints in chromosome 2, which to our knowledge, have not yet been reported in liver cells.

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Studies on the Methylation of Avian Sarcoma Proviruses in Permissive and Non-permissive Cells

Cited by 20 publications

References 20 publications

Alterations in the transport and processing of Rous sarcoma virus envelope glycoproteins mutated in the signal and anchor regions

Alterations in the transport and processing of Rous sarcoma virus envelope glycoproteins mutated in the signal and anchor regions

Ducks: a new experimental host system for studying persistent infection with avian leukaemia retroviruses

Isolation and characterization of a human hepatic epithelial-like cell line (AKN-1) from a normal liver

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