Studies on the Metabolism of Rat-Ascites-Tumors with Nitrogen Mustard Sensitive and Resistant Strains

Abstract

Since the development of the subcellular fractionation method, the fact that many en zymes are unequally distributed in the cells has now been firmly established by several investigators. Among them, C. de Duve and his group (1) have already reported intracellu lar distribution patterns of enzymes in normal rat liver tissues. This paper is a comparative study on the intracellular distribution patterns of some en zymes with normal livers, regenerating livers and two strains of rat-ascites-hepatoma. These patterns were different for normal and regene rating livers as well as for two strains of DABhepatoma. However, the greatest difference was generally observed between normal liver and hepatoma even after taking account of the fundamental difference in the mass of the nuclei or mitochondria of those two tissues.