Studies on the mechanism of multidrug resistance of Acinetobacter baumannii by proteomic analysis of the outer membrane vesicles of the bacterium

Agarwal, Bina; Karthikeyan, Raman; Gayathri, Pratapa; RameshBabu, B.; Ahmed, Giasuddin; Jagannadham, Medicharla V.

doi:10.1007/s42485-018-0001-4

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…This indicates that inhibition of MV release and anti-bacterial action are likely linked, as previously suggested (Tashiro et al, 2010). Indeed, a recent study using indole derivatives has revealed a role for MVs in antibiotic resistance/persistence, in particular in Gram-negative bacteria tested (Agarwal et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles

Kosgodage

Matewele

Awamaria

et al. 2019

Front. Cell. Infect. Microbiol.

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Membrane vesicles (MVs) released from bacteria participate in cell communication and host-pathogen interactions. Roles for MVs in antibiotic resistance are gaining increased attention and in this study we investigated if known anti-bacterial effects of cannabidiol (CBD), a phytocannabinoid from Cannabis sativa, could be in part attributed to effects on bacterial MV profile and MV release. We found that CBD is a strong inhibitor of MV release from Gram-negative bacteria (E. coli VCS257), while inhibitory effect on MV release from Gram-positive bacteria (S. aureus subsp. aureus Rosenbach) was negligible. When used in combination with selected antibiotics, CBD significantly increased the bactericidal action of several antibiotics in the Gram-negative bacteria. In addition, CBD increased antibiotic effects of kanamycin in the Gram-positive bacteria, without affecting MV release. CBD furthermore changed protein profiles of MVs released from E. coli after 1 h CBD treatment. Our findings indicate that CBD may pose as a putative adjuvant agent for tailored co-application with selected antibiotics, depending on bacterial species, to increase antibiotic activity, including via MV inhibition, and help reduce antibiotic resistance.

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Section: Discussionmentioning

confidence: 99%

Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles

Kosgodage

Matewele

Awamaria

et al. 2019

Front. Cell. Infect. Microbiol.

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“…Numerous Gram-negative bacteria produce OMVs, which are enriched in host invasion-related effectors or proteins, which neutralize antibiotics, such as β-lactamases [44]. The production of OMVs increases when antibiotics are present in the growth media [45] and the ability of E. coli OMVs to inactivate β-lactams have been demonstrated [46]. The C-terminus of A. baumannii OmpA protein has been shown to be important for the resistance to antibiotics such as aztreonam, ciprofloxacin, colistin, gentamicin, trimethoprim and imipenem [18].…”

Section: Discussionmentioning

confidence: 99%

The Mutation of Conservative Asp268 Residue in the Peptidoglycan-Associated Domain of the OmpA Protein Affects Multiple Acinetobacter baumannii Virulence Characteristics

et al. 2019

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Acinetobacter baumannii is a nosocomial human pathogen of increasing concern due to its multidrug resistance profile. The outer membrane protein A (OmpA) is an abundant bacterial cell surface component involved in A. baumannii pathogenesis. It has been shown that the C-terminal domain of OmpA is located in the periplasm and non-covalently associates with the peptidoglycan layer via two conserved amino acids, thereby anchoring OmpA to the cell wall. Here, we investigated the role of one of the respective residues, D268 in OmpA of A. baumannii clinical strain Ab169, on its virulence characteristics by complementing the ΔompA mutant with the plasmid-borne ompAD268A allele. We show that while restoring the impaired biofilm formation of the ΔompA strain, the Ab169ompAD268A mutant tended to form bacterial filaments, indicating the abnormalities in cell division. Moreover, the Ab169 OmpA D268-mediated association to peptidoglycan was required for the manifestation of twitching motility, desiccation resistance, serum-induced killing, adhesion to epithelial cells and virulence in a nematode infection model, although it was dispensable for the uptake of β-lactam antibiotics by outer membrane vesicles. Overall, the results of this study demonstrate that the OmpA C-terminal domain-mediated association to peptidoglycan is critical for a number of virulent properties displayed by A. baumannii outside and within the host.

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“…This interaction could regulate how bacteria produce outer membrane vesicles (OMVs) and preserve the stability of the membrane [ 41 ]. In order to combat antibiotic resistance, OMVs with OmpA in their membrane aggressively drain extracellular drugs [ 42 , 43 , 44 ].…”

Section: Ompamentioning

confidence: 99%

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Zhou

Wang

et al. 2023

Microorganisms

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Gram-negative bacteria depend on their cell membranes for survival and environmental adaptation. They contain two membranes, one of which is the outer membrane (OM), which is home to several different outer membrane proteins (Omps). One class of important Omps is porins, which mediate the inflow of nutrients and several antimicrobial drugs. The microorganism’s sensitivity to antibiotics, which are predominantly targeted at internal sites, is greatly influenced by the permeability characteristics of porins. In this review, the properties and interactions of five common porins, OmpA, OmpC, OmpF, OmpW, and OmpX, in connection to porin-mediated permeability are outlined. Meanwhile, this review also highlighted the discovered regulatory characteristics and identified molecular mechanisms in antibiotic penetration through porins. Taken together, uncovering porins’ functional properties will pave the way to investigate effective agents or approaches that use porins as targets to get rid of resistant gram-negative bacteria.

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Studies on the mechanism of multidrug resistance of Acinetobacter baumannii by proteomic analysis of the outer membrane vesicles of the bacterium

Cited by 7 publications

References 43 publications

Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles

Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles

The Mutation of Conservative Asp268 Residue in the Peptidoglycan-Associated Domain of the OmpA Protein Affects Multiple Acinetobacter baumannii Virulence Characteristics

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

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