Studies on the local (paracrine) actions of glucagon, somatostatin and insulin in isolated islets of rat pancreas

Schatz, H.; Kullek, U.

doi:10.1016/0014-5793(80)80439-x

Cited by 19 publications

(8 citation statements)

References 20 publications

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“…Moreover, the importance of the microvascular organization, which permitted sequential perfusion of the different cell types, has been recognized in the functional regulation of in situ pancreatic islets (Samols et al 1988;Menger et al 1994). This condition supports both a paracrine action (Jörns et al 1988;Schatz & Kullek, 1980) and an endocrine action through blood flow inside pancreatic islets (Kawai et al 1995) mediated by the insular hormones.…”

Section: Discussionsupporting

confidence: 67%

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Revascularization and remodelling of pancreatic islets grafted under the kidney capsule

et al. 2007

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The revascularization and the structural changes resulting from interactions between the graft and the host were investigated in transplanted pancreatic islets under the kidney capsule. Islets were isolated from mice pancreata and transplanted in syngeneic diabetic animals. Graft-bearing kidneys were collected on different days posttransplant and processed for light microscopy, immunohistochemistry and transmission electron microscopy. A numerical analysis was performed in order to compare the percentage number of the different types of cells in native islets and at different time points after the transplant. Recipient animals reversed diabetes within 4 days.An intraperitoneal glucose tolerance test was performed to determine islet functionality under stressful conditions.During the initial few days post-transplant, the islets showed peculiar shapes and the graft tended to aggregate along the vessels. Starting at days 4 -7 post-transplant, islets were revascularized from vessels connected to both the cortical and the capsular vascular network of the kidney. From day 7-14 post-transplant, the vessels progressively appeared more similar in features and size to those of in situ pancreatic islets. Both the percentage number of the different cell types and the distribution of Alpha, Beta and Delta cells inside the graft were significantly different as compared with intact islets, demonstrating quantitative and structural changes after the engraftment. No concomitant proliferation of Beta cells was detected using a bromodeoxyuridin staining method. Despite the fact that quick revascularization preserved a large mass of tissue, the remodelling process of the graft and the newly formed vascularization led to a different organization of the endocrine tissue as compared with intact in situ islets. This constitutes the morphological basis for alterations of the normal intercellular interactions and may explain the altered secretory cell function often observed in transplant.

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Section: Discussionsupporting

confidence: 67%

“…This condition supports both a paracrine action (Jörns et al. 1988; Schatz & Kullek, 1980) and an endocrine action through blood flow inside pancreatic islets (Kawai et al. 1995) mediated by the insular hormones.…”

Section: Discussionsupporting

confidence: 64%

Revascularization and remodelling of pancreatic islets grafted under the kidney capsule

et al. 2007

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“…On the other hand, Chen et al (2007) recently showed that resveratrol increased insulin secretion from different β-cell insulinoma lines (MIN6, Hit-T15 and RIN-m5F). Such a distinction between whole islets and isolated β-cells could partially result from paracrine interactions within islets involving augmented secretion of somatostatin (Schatz and Kullek, 1980;Samols et al, 1986). The suppression of somatostatin action in islets canceled the inhibitory influence of 1 µM resveratrol.…”

Section: Discussionmentioning

confidence: 97%

The insulin-suppressive effect of resveratrol — An in vitro and in vivo phenomenon

Szkudelski¹

2008

Life Sciences

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“…Raised glucagon levels in calcium-free medium (LeclercqMeyer et al 1975;but cf. Lundquist et al 1976) might explain elevated (pro-)insulin biosynthesis under these conditions, since it has been shown that both exogeneous (Schatz et al 1973) and endogeneous (Schatz & Kullek 1980) glucagon stimulate insulin synthesis.…”

Section: Discussionmentioning

confidence: 94%

Long-term and short-term effects of calcium, verapamil and diazoxide on biosynthesis and release of (pro-)insulin in isolated islets of rat pancreas

Leinweber¹,

Schatz²

1982

Acta Endocrinologica

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Glucose-induced release and biosynthesis of insulin can be influenced independently during short\x=req-\ term incubations of isolated pancreatic islets. We studied whether such a dissociation between synthesis and release is still retained after a longer period of islet culture. Media varying in calcium concentration (0, 0.8, 2.5, 5.0 mM), verapamil (8 \g=m\m) or diazoxide (100 \g=m\g/ml) were used in short-term incubations for 2 h and during 38 h of islet culture at 2 mg/ml glucose. In the short-term experiments, the increase in calcium concentration caused a stepwise augmentation of insulin release but a gradual decrease in (pro-)insulin synthesis; verapamil and diazoxide markedly diminished insulin secretion, whereas hormone biosynthesis was found unchanged. Culture of islets with calcium omission, verapamil or diazoxide resulted also in a reduced insulin release during the 38 h of cultivation. After culture, insulin release was not different from the controls any more. (Pro-)Insulin biosynthesis, however, was now significantly diminished in islets cultured before with verapamil and diazoxide; following culture in calcium-free medium biosynthesis was also relatively decreased compared to the values obtained in the acute short-term experiments. It appears from these results that the pharmacological inhibitors of insulin release tested in vitro do no longer influence release and biosynthesis of insulin independently after a longer culture period, in contrast to their short-term effects.

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Studies on the local (paracrine) actions of glucagon, somatostatin and insulin in isolated islets of rat pancreas

Cited by 19 publications

References 20 publications

Revascularization and remodelling of pancreatic islets grafted under the kidney capsule

Revascularization and remodelling of pancreatic islets grafted under the kidney capsule

The insulin-suppressive effect of resveratrol — An in vitro and in vivo phenomenon

Long-term and short-term effects of calcium, verapamil and diazoxide on biosynthesis and release of (pro-)insulin in isolated islets of rat pancreas

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