Studies on the joint cytotoxicity of Wheat Germ Agglutinin and monensin

Pellegrina, Chiara Dalla; Matucci, Andrea; Zoccatelli, Gianni; Rizzi, Corrado; Vincenzi, Simone; Veneri, Gianluca; Andrighetto, G.; Peruffo, A. Dal Belin; Chignola, Roberto

doi:10.1016/j.tiv.2004.04.008

Cited by 12 publications

(16 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…WGA exerted cytotoxic effects, to different extents depending on the cell line tested, in toxicity assays [28]. In this study, WGA induced the most pronounced cell death of all three lectins tested (Figure 3).…”

Section: Resultsmentioning

confidence: 61%

Detection of Glycomic Alterations Induced by Overexpression of P-Glycoprotein on the Surfaces of L1210 Cells Using Sialic Acid Binding Lectins

Bubencíkova

Cholujová

Messingerová

et al. 2012

IJMS

View full text Add to dashboard Cite

P-glycoprotein (P-gp) overexpression is the most frequently observed cause of multidrug resistance in neoplastic cells. In our experiments, P-gp was expressed in L1210 mice leukemia cells (S cells) by selection with vincristine (R cells) or transfection with the gene encoding human P-gp (T cells). Remodeling of cell surface sugars is associated with P-gp expression in L1210 cells as a secondary cellular response. In this study, we monitored the alteration of cell surface saccharides by Sambucus nigra agglutinin (SNA), wheat germ agglutinin (WGA) and Maackia amurensis agglutinin (MAA). Sialic acid is predominantly linked to the surface of S, R and T cells via α-2,6 branched sugars that tightly bind SNA. The presence of sialic acid linked to the cell surface via α-2,3 branched sugars was negligible, and the binding of MAA (recognizing this branch) was much less pronounced than SNA. WGA induced greater cell death than SNA, which was bound to the cell surface and agglutinated all three L1210 cell-variants more effectively than WGA. Thus, the ability of lectins to induce cell death did not correlate with their binding efficiency and agglutination potency. Compared to S cells, P-gp positive R and T cells contain a higher amount of N-acetyl-glucosamine on their cell surface, which is associated with improved WGA binding. Both P-gp positive variants of L1210 cells are strongly resistant to vincristine as P-gp prototypical drug. This resistance could not be altered by liberalization of terminal sialyl residues from the cell surface by sialidase.

show abstract

Section: Resultsmentioning

confidence: 61%

Detection of Glycomic Alterations Induced by Overexpression of P-Glycoprotein on the Surfaces of L1210 Cells Using Sialic Acid Binding Lectins

Bubencíkova

Cholujová

Messingerová

et al. 2012

IJMS

View full text Add to dashboard Cite

show abstract

“…Lectins interact with specific glycan structures linked to soluble and membrane-bound glycoconjugates, and these proteincarbohydrate interactions play key roles in biological processes such as cell communication, pathogen infection and host defense, fertilization, development, cell differentiation, cancer, and metastasis (Damme et al 1998, Beisel et al 1999. There is evidence that land plant lectins present antitumoral activity against human cancer lines (Dalla Pellegrina et al 2004, Park et al 2004, Chauhan et al 2005, Khil et al 2007; however, in this study the aqueous extract containing hydrophilic compounds such as lectins did not show important activity, suggesting that lectins could not be responsible for the cytotoxic and antiproliferative activity of B. triquetum against cancer cells. More studies are necessary to isolate and identify active compounds responsible for those effects.…”

Section: Rhodophytamentioning

confidence: 99%

In vitro cytotoxic and antiproliferative activities of marine macroalgae from Yucatán, Mexico

2009

View full text Add to dashboard Cite

Extracts from 27 marine algal species (14 Rhodophyta, 5 Phaeophyta, and 8 Chlorophyta) from the Yucatán Peninsula (Mexico) were evaluated for cytotoxic and antiproliferative activity by 3(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sulforhodamine B (SRB) assays, respectively. To determine the specificity of cytotoxic activity against tumor cells, the selective index (SI) was also calculated. The following cancer cell lines were employed: normal canine kidney (MDCK) cells, human laryngeal carcinoma (Hep-2) cells, human cervical adenocarcinoma (HeLa) cells, and human nasopharyngeal carcinoma (KB) cells. The results indicated that 44% and 51% of the algal species tested showed cytotoxic and antiproliferative activity, respectively. Most of the cytotoxic extracts were from species of Chlorophyta, with Udotea flabellum and U. conglutinate showing the highest cytotoxic activity against all the cancer cell lines. For Rhodophyta, the Bryothamnion triquetrum extract showed outstanding selective cytotoxicity against Hep-2 cells (CC 50 8.29 µg mL-1 , SI = 12.04). Two of the five species of Phaeophyta tested (Lobophora variegata and Dictyota caribaea) showed high cytotoxicity activity against the KB cell line. The data show that these species are a potential source of compounds for the treatment of certain cancer diseases.

show abstract

“…Cells were cultured at 37°C in a 5% CO 2 humidified atmosphere and passaged weekly. Human peripheral mononuclear cells were drawn from healthy volunteers as described previously [6].…”

Section: Cell Lines and Cytofluorimetric Assaysmentioning

confidence: 99%

“…Cells from exponentially growing cultures were labelled with ethidium bromide and analysed as described [6] using an EPICS-X (Coulter, Hialeah, FL) cytofluorimeter.…”

Section: Cell Lines and Cytofluorimetric Assaysmentioning

confidence: 99%

A phenomenological approach to the simulation of metabolism and proliferation dynamics of large tumour cell populations

2005

Self Cite

View full text Add to dashboard Cite

A major goal of modern computational biology is to simulate the collective behaviour of large cell populations starting from the intricate web of molecular interactions occurring at the microscopic level. In this paper we describe a simplified model of cell metabolism, growth and proliferation, suitable for inclusion in a multicell simulator, now under development (Chignola R and Milotti E 2004 Physica A 338 261-6). Nutrients regulate the proliferation dynamics of tumour cells which adapt their behaviour to respond to changes in the biochemical composition of the environment. This modelling of nutrient metabolism and cell cycle at a mesoscopic scale level leads to a continuous flow of information between the two disparate spatiotemporal scales of molecular and cellular dynamics that can be simulated with modern computers and tested experimentally.

show abstract

Studies on the joint cytotoxicity of Wheat Germ Agglutinin and monensin

Cited by 12 publications

References 17 publications

Detection of Glycomic Alterations Induced by Overexpression of P-Glycoprotein on the Surfaces of L1210 Cells Using Sialic Acid Binding Lectins

Detection of Glycomic Alterations Induced by Overexpression of P-Glycoprotein on the Surfaces of L1210 Cells Using Sialic Acid Binding Lectins

In vitro cytotoxic and antiproliferative activities of marine macroalgae from Yucatán, Mexico

A phenomenological approach to the simulation of metabolism and proliferation dynamics of large tumour cell populations

Contact Info

Product

Resources

About