Studies on the Ion‐Exchange Chromatographic Separation of L‐Ascorbic Acid and Oxalic Acid

Abstract

L‐Ascorbic acid and oxalic acid could be successfully separated with a column of 8% cross‐linked sulfonated polystyrene cation‐exchange resin in hydrogen form, and eluted with 0.01% aqueous solution of nitric acid, sulfuric acid, or phosphoric acid. Among the mineral acids used, nitric acid showed the best separatory effect. The minimum detectable quantities of the acids were 0.05 μg and 0.5 μg per 0.5 ml sample solution for L‐ascorbic acid and oxalic acid, respectively. Under this condition, the acids could be detected by using automatic UV‐monitor apparatus.