Studies on the interactions of a novel ruthenium(II) complex with G-quadruplex DNA

Abstract

A novel ruthenium(II) porphyrin complex, [Ru(phen) 2 MPyTPP] (phen = phenanthroline, MPyTPP = mono-(3 0 -Pyridine)-10,15,20-triphenyl porphyrin), has been prepared and characterized by elemental analysis, ESI-MS and NMR methods. The interaction of the complex with G-quadruplex DNA has been studied by spectroscopy, and the results have shown that [Ru(phen) 2 MPyTPP] can bind to G-quadruplex d(TTAGGG) 4 with high affinity. In the presence of d(TTAGGG) 4 , the characteristic Soret and intra-ligand (IL) transitions in the UV-Vis spectrum of the complex exhibit hypochromism of 25% (Dk = 6 nm) and 18% (Dk = 1 nm), respectively. The intrinsic binding constant was calculated according to the decay of the Soret band as 3.02(±0.08) 9 10 6 M -1 . The positive signal of d(TTAGGG) 4 in its circular dichroism spectra was clearly decreased in the presence of the complex, indicating the conformation of the G-quadruplex was severely disturbed by the ruthenium(II) complex.

Wechselwirkung von Metallkomplexen mit G‐Quadruplex‐DNA

“…In einer solchen Verbindung (Abbildung 15 a) wurde ein Pyridinligand mit einem Porphyrinring verknüpft,45 um die Lipophilie und damit die Bioverfügbarkeit zu erhöhen. Dieser Komplex bindet mit so hoher Affinität an den H‐Telo‐Quadruplex, dass dessen Parallelkonformation zerstört wird.…”

“… Einige oktaedrisch koordinierte Ruthenium(II)‐Komplexe, die über eine Kombination aus π‐Stapelung der Liganden und elektrostatischen Wechselwirkungen mit Furchen oder Schleifen an DNA‐Quadruplexe binden. a) [RuCl(phen) 2 (MPyTPP)] + 45. b) Lumineszierende Zweikernkomplexe mit aromatischen Diiminliganden44a und c) ihre einkernigen Analoga 34.…”

Synthesis and characterization of chiral ruthenium(II) complexes Λ/Δ-[Ru(bpy)2(H2iip)](ClO4)2 as stabilizers of c-myc G-quadruplex DNA

“…Recently, evidence has shown that ruthenium (II) complexes can also act as potential intercalators of G-quadruplex [11,12]. For example, ruthenium complex with polypridine has high affinity with G-quadruplex [13].…”

Antibacterial effect of cationic porphyrazines and anionic phthalocyanine and their interaction with plasmid DNA

“…The concentrations of Cu(2,3-tmtppa), Cu (3,4-tmtppa) and Cu(PcTs) was determined using molar extinction coefficient values of 1. [14]. To determine the quenching constant, Stern-Volmer equation (2) and its modified form (3) was applied [15].…”