Studies on the interaction of human erythrocyte band 3 with membrane lipids using deuterium nuclear magnetic resonance and differential scanning calorimetry

Morrow, Michael R.; Davis, James H.; Sharom, Frances J.; Lamb, Mary P.

doi:10.1016/0005-2736(86)90286-5

Cited by 24 publications

(17 citation statements)

References 27 publications

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“…Left: Cytochrome b 5 lowers the melting point (from [13] adapted from [86]). Right: Band 3 protein of erythrocytes increases the melting point (from [13] adapted from [87]).…”

Section: Proteins As Membrane Perturbationsmentioning

confidence: 99%

Lipid ion channels

Heimburg

2010

Biophysical Chemistry

148

178

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The interpretation of electrical phenomena in biomembranes is usually based on the assumption that the experimentally found discrete ion conduction events are due to a particular class of proteins called ion channels while the lipid membrane is considered being an inert electrical insulator. The particular protein structure is thought to be related to ion specificity, specific recognition of drugs by receptors and to macroscopic phenomena as nerve pulse propagation. However, lipid membranes in their chain melting regime are known to be highly permeable to ions, water and small molecules, and are therefore not always inert. In voltage-clamp experiments one finds quantized conduction events through protein-free membranes in their melting regime similar to or even undistinguishable from those attributed to proteins. This constitutes a conceptual problem for the interpretation of electrophysiological data obtained from biological membrane preparations. Here, we review the experimental evidence for lipid ion channels, their properties and the physical chemistry underlying their creation. We introduce into the thermodynamic theory of membrane fluctuations from which the lipid channels originate. Furthermore, we demonstrate how the appearance of lipid channels can be influenced by the alteration of the thermodynamic variables (e.g., temperature, pressure, tension and chemical potentials) in a coherent description that is free of parameters. This description leads to pores that display dwell times closely coupled to the fluctuation lifetime via the fluctuation-dissipation theorem. Drugs as anesthetics and neurotransmitters are shown to influence the channel likelihood and their lifetimes in a predictable manner. We also discuss the role of proteins in influencing the likelihood of lipid channel formation.

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“…Left: Cytochrome b 5 lowers the melting point (from [13] adapted from [86]). Right: Band 3 protein of erythrocytes increases the melting point (from [13] adapted from [87]).…”

Section: Proteins As Membrane Perturbationsmentioning

confidence: 99%

Lipid ion channels

Heimburg

2010

Biophysical Chemistry

148

178

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“…1983; Morrow et al. 1986; Heimburg 2007). These facts are used to propose a protein-mediated membrane perturbation mechanism via hydrophobic mismatch that would create small belts of highly fluctuating, defect-prone lipids at the interface with proteins (Heimburg 2010).…”

Section: Intrinsic Leakage Of Membranesmentioning

confidence: 99%

A lipocentric view of peptide-induced pores

et al. 2011

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Although lipid membranes serve as effective sealing barriers for the passage of most polar solutes, nonmediated leakage is not completely improbable. A high activation energy normally keeps unassisted bilayer permeation at a very low frequency, but lipids are able to self-organize as pores even in peptide-free and protein-free membranes. The probability of leakage phenomena increases under conditions such as phase coexistence, external stress or perturbation associated to binding of nonlipidic molecules. Here, we argue that pore formation can be viewed as an intrinsic property of lipid bilayers, with strong similarities in the structure and mechanism between pores formed with participation of peptides, lipidic pores induced by different types of stress, and spontaneous transient bilayer defects driven by thermal fluctuations. Within such a lipocentric framework, amphipathic peptides are best described as pore-inducing rather than pore-forming elements. Active peptides bound to membranes can be understood as a source of internal surface tension which facilitates pore formation by diminishing the high activation energy barrier. This first or immediate action of the peptide has some resemblance to catalysis. However, the presence of membrane-active peptides has the additional effect of displacing the equilibrium towards the pore-open state, which is then maintained over long times, and reducing the size of initial individual pores. Thus, pore-inducing peptides, regardless of their sequence and oligomeric organization, can be assigned a double role of increasing the probability of pore formation in membranes to high levels as well as stabilizing these pores after they appear.

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“…It is reasonable to suppose that the nature of the membrane proteins would have an effect, as would the membrane lipid/protein compositional ratio; in some cases biological membranes can contain up to 60-70% protein, so the protein presence would be likely to make a substantial contribution to the overall nature of the bilayer. In fact, previous studies have shown small but definite effects of intrinsic proteins on the gel-fluid transition [194-196], and on the lamellar-hexagonal transition [197]. Data extracted from lipid-only bilayer model systems have demonstrated that both the presence and the physical characteristics of phase coexistence are highly dependent on the lipid composition of the membrane itself and the particular environmental conditions such as the temperature.…”

Section: The Uncertaintiesmentioning

confidence: 99%

Phase diagrams of lipid mixtures relevant to the study of membrane rafts

Goñi

Alonso

Bagatolli

et al. 2008

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

198

128

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The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, and detergent solubilization assays, are critically evaluated and rationalized in terms of triangular phase diagrams. The remaining uncertainties are discussed specifically and separately from the data on which a consensus appears to exist.

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Studies on the interaction of human erythrocyte band 3 with membrane lipids using deuterium nuclear magnetic resonance and differential scanning calorimetry

Cited by 24 publications

References 27 publications

Lipid ion channels

Lipid ion channels

A lipocentric view of peptide-induced pores

Phase diagrams of lipid mixtures relevant to the study of membrane rafts

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