Studies on the induction and expression of T cell-mediated immunity. XII. The concomitant loss and recovery of membrane-associated Lyt-2 antigens, lymphocyte-target cell binding, and the antigen-specific and -nonspecific cytotoxic activity of alloimmune T lymphocytes after treatment with trypsin.

Fan, John; Bonavida, B

doi:10.4049/jimmunol.127.5.1856

Cited by 23 publications

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Selective inhibition of antigen‐induced “step one” in cytotoxic T lymphocytes by anti‐Lyt‐2 antibodies

Gullberg

Larsson

1982

Eur J Immunol

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Stimulation of mixed lymphocyte cultures with ultraviolet (UV)-irradiated stimulator cells leads to selective activation of Lyt-2+ cytotoxic T lymphocyte precursors (CTL-P) to acquire interleukin 2 (IL2) reactivity: (a) no proliferative response, nor IL2 production was observed in these cultures; (b) upon addition of preformed IL2-conditioned media (IL2-CM), a vigorous proliferative response was observed; (c) the IL2-dependent proliferation was reduced by approximately 90% after depletion of Lyt-2+ responder cells; and (d) specific CTL were generated upon the initial triggering by UV-irradiated stimulators and IL2 expansion. This system therefore provides suitable conditions for studying the role of Lyt-2 antigens in initial, antigen-specific induction of CTL-P, as well as at their effector phase. It is shown herein that monoclonal anti-Lyt-2 antibodies inhibit antigen-specific acquisition of IL2 reactivity by CTL-P, which results in 85% reduction of the proliferative response mediated by IL2-CM. CTL-P which have been induced by antigen to IL2 reactivity, prior to addition of anti-Lyt-2 antibodies, however, are not affected in their IL2-dependent proliferation. Furthermore, both the specific and lectin-dependent cytolytic activity of effector CTL generated by UV-irradiated stimulators and IL2-CM is blocked by addition of anti-Lyt-2 antibodies in the cytotoxicity assay. The implications of these findings for the role of Lyt-2 antigen in antigen recognition by T cells are discussed.

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Selective inhibition of antigen‐induced “step one” in cytotoxic T lymphocytes by anti‐Lyt‐2 antibodies

Gullberg

Larsson

1982

Eur J Immunol

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Induction of cytolytic T lymphocytes by mitogenic lectins is specifically inhibited by anti‐Lyt‐2 antibodies

Gullberg

Larsson

1983

Eur J Immunol

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The effects of anti-Lyt-2 antibodies at different levels of lectin-induced cytotoxic T lymphocyte (CTL) responses have been analyzed. Induction of interleukin 2 (T cell growth factor) reactivity ("step 1") by three different lectins was profoundly inhibited by anti-Lyt-2 antibodies. Also lectin-dependent cytotoxicity of polyclonally induced CTL was significantly inhibited by anti-Lyt-2 antibodies, whereas the interjacent IL2-dependent proliferation of "step 1"-induced CTL precursors was unaffected by these antibodies. The anti-Lyt-2-mediated inhibition was selective and specific to the target cell determinants and limited to the events in CTL responses which require interactions with the lectins. These results parallel those obtained in antigen-induced responses and indicate that antigens and lectins induce CTL responses by interacting with the same type of membrane receptor complex containing Lyt-2 molecules and antigen-specific receptors. It follows that T cell mitogenic lectins do not appear to deliver triggering signals through "mitogen" receptors.

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The Molecular Basis for Cytolytic T Lymphocyte Function: Analysis with Blocking Monoclonal Antibodies

Martz

Davignon

Kürzinger

et al. 1982

Advances in Experimental Medicine and Biology

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Studies on the induction and expression of T cell-mediated immunity. XII. The concomitant loss and recovery of membrane-associated Lyt-2 antigens, lymphocyte-target cell binding, and the antigen-specific and -nonspecific cytotoxic activity of alloimmune T lymphocytes after treatment with trypsin.

Cited by 23 publications

References 0 publications

Selective inhibition of antigen‐induced “step one” in cytotoxic T lymphocytes by anti‐Lyt‐2 antibodies

Selective inhibition of antigen‐induced “step one” in cytotoxic T lymphocytes by anti‐Lyt‐2 antibodies

Induction of cytolytic T lymphocytes by mitogenic lectins is specifically inhibited by anti‐Lyt‐2 antibodies

The Molecular Basis for Cytolytic T Lymphocyte Function: Analysis with Blocking Monoclonal Antibodies

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