Studies on the in vitro behavior of agammaglobulinemic lymphocytes

Abstract

A B S T R A C T Circulating lymphocytes

Biosynthesis <i>in vitro</i> of Immunoglobulins in Primary Immunological Deficiencies

“…On the contrary, other experiences with long-term lymphocyte cultures derived from patients with hypogammaglobulinaemia constantly showed a synthesis of IgG and IgM [7,15]. These researchers maintain that lym phocytes do not retain the biochemical abnormalities of the cell donors, and that the pathogenesis would probably appear in cells other than cir culating lymphocytes.…”

“…In addition, the lymphocytes of these patients in long-term cultures synthesized immunoglobulins; short term cultures were negative. This behaviour suggests that the pathogene sis of the deficiency should occur in cells other than circulating lympho cytes [7,15].In an attempt to gain insight into the pathogenesis of the primary im munological defect, we studied the biosynthesis in vitro of immunoglobu lins of different tissues of patients with agammaglobulinaemia and dys gammaglobulinaemia. …”

The Use of Transfer Factor in a Patient with Agammaglobulinemia

“…It has been shown that the peripheral blood lymphocytes of some patients with congenital aggamaglobulinemia have the capacity to synthesize normal amounts of y-globulin when stimulated in short term culture (8,25). Similarly, long term lymphoid cell lines, derived from patients with hypogammaglobulinemia have the capacity to synthesize immunoglobulins in vitro (25).…”

In Vitro Responses of Lymphocytes from Cancer-Bearing Patients to Autochthonous Tumor Tissues

“…Tissue culture experiments have generally been too demanding for extensive investigation, although recently a (8,9,(14)(15)(16). A number of studies have recently demonstrated that peripheral blood lymphocyte transformation and proliferation in tissue culture, induced by antigens, is primarily a manifestation of cellular immunity (10,11). We have, therefore, attempted to use this experimental system to demonstrate that a delayed hypersensitivity reaction occurs in man against tumor-specific antigens, We have used autochthonous tumor tissue homogenates to induce proliferation in tissue cultures of the patients o m blood lymphocytes.…”

“…On the day of the lymphocyte culture, the tissues were thawed, weighed, homogenized with a Potter-Elvehjem glass tissue grinder, and suspended in incubation media at a concentration of 3 mg/ml. Lymphocyte cultures were established as previously described (11) with medium 199 containing 10% fetal calf serum. One-ml cul- tablished and the cultures were maintained in water-saturated 5 % C02-95% air at 36" for 7 days.…”