Abstract:together with 2 ml of 0.1N HC1 for 15 minutes.After we discarded the ethyl acetate layer, the residual HC1-layers were alkalinized again at a pH above 7.8 with 0.3N NaOH and were again extracted three times with ethyl acetate.The ethyl acetate layers containing metyrapone, reduced metyrapone and the internal standard were evaporated to dryness with a stream of nitrogen in a 37°C water bath.Following the above procedures, reduced metyrapone was converted to a trimethylsilyl (TMS) derivative of reduced metyrapon… Show more
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