Studies on the human T cell receptor α/β variable region genes. I. Identification of 7 additional V<sub>α</sub> subfamilies and 14 J<sub>α</sub> gene segments

Roman‐Roman, Sergio; Ferradini, Laurent; Azócar, José; Genevée, Catherine; Hercend, Thierry; Triebel, Frédéric

doi:10.1002/eji.1830210411

Cited by 116 publications

(43 citation statements)

References 35 publications

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“…AP511 has been described to contain an in-frame stop codon but, in our analysis, there is a point mutation that has changed this segment into a functional one. Ja33 to Ja36, Ja38, Ja4l, and Ja42 correspond to IGRJaO8, IGRJa12, IGRJaO3, IGRJaO5, IGRJaOlC, IGRUaM4, and IGRJaO4 (38); Ja37, Ja39, and Ja40 have not been described, to our knowledge. The V81 gene segment has been described (39).…”

Section: Resultsmentioning

confidence: 65%

The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.

Uematsu

Wege

Straus

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

148

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We have analyzed the T-cell-receptor repertoire expressed in the synovial fluid of a patient with rheumatoid arthritis by using an inverse polymerase chain reaction. Total RNA was isolated from Ficoll-purifled mononuclear cells and converted into circularized double-stranded cDNA. Specific amplification of a-and E-chain variable regions (Va and VP) was achieved with inverted a-and 3-chain constant region (Ca and C13) primer pairs, and the amplification products were cloned into phage vectors. A total of 78 a and 7618 clones were sequenced, and 67 and 72 productively rearranged a and 13 genes were identified, respectively. Thirty-one Va, 33 a-chain joining region (Ja), 29 VP3, and 12 13-chain joining region (J13) gene segments were found in the productively rearranged clones, indicating that the T-cell repertoire expressed in the synovial fluid of this RA patient is highly heterogenous and polyclonal. Comparison of peripheral blood and synovial fluid repertoires showed that the most abundant V13 sequences, V132.1 and V133

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Section: Resultsmentioning

confidence: 65%

The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.

Uematsu

Wege

Straus

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

148

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“…Va21 sequences differed at residues 75 and 76, a change that reintroduces the highly conserved leucine at position 75 [MI. In Va29 two new variants were detected at positions 57-60 [21].The WR and JM Va29 differed by four nucleotides from the previously reported sequence; the AJ variant had only three changes. Finally, subject JM apparently has two variants of Va28, which differed at position 87 [21].…”

Section: New V a Sequencesmentioning

confidence: 63%

Characterization of the human T cell receptor α‐chain repertoire and demonstration of a genetic influence on Vα usage

et al. 1993

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Despite considerable knowledge of the determinants and profile of the T cell receptor (TCR) beta-chain repertoire, very little is known about the repertoire of the V alpha and J alpha segments of the TCR in man or mouse. We have used the anchored polymerase chain reaction (PCR) to analyze the human TCR alpha-chain repertoire at the mRNA level in peripheral blood lymphocytes. We find marked differences in the usage of individual V alpha segments. The V alpha repertoire is determined both by differences in the use of individual V alpha gene segments at the time of gene rearrangement and by genetic influences on the selection of the expressed V alpha repertoire, presumably during thymic maturation. Analysis of the J alpha repertoire shows non-random usage of individual J alpha segments but no obvious relationship to chromosomal position. We also report three new V alpha segments, three new J alpha segments and possible allelic variation in V alpha gene segments. Two unusual TCR alpha transcripts are described which add new information on the nature of TCR alpha transcript assembly.

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“…2 a. The a chain expression of these clones was also determined, by sequencing the products of a chain-specific anchor PCR. Once more the five clones fell into two categories: the Vp5.2-expressing clones MAW1.4, 1.5 and 1.8 each expressed a member of the Va2 family highly related toVa2.3 [42] which we have called Va2.4,whereas theVP5.6-expressing clones MAW10.8 and 10.15 expressed a member of the Vaw23 family, [43] which we have designated Vaw23.2. In the case of MAW10.8, shown to be biclonal with respect to P chain expression, a second a chain was also detected.…”

Section: Dr3-restricted T Cell Clones Preferentially Express a Vps Famentioning

confidence: 99%

Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65‐kDa heat‐shock protein: Selective in vivo expansion of T cells bearing defined receptors

et al. 1993

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We have examined the T cell receptor (TcR) expression of clones specific for epitopes of mycobacterial 65-kDa heat-shock protein (hsp65) in the context of two different HLA molecules, and used this system as a model to assess the selection of T cells responsive to this antigen in vivo. DR3-restricted clones were raised from both the synovial fluid (SF) and peripheral blood (PB) of a patient with reactive arthritis in three separate cloning events. Five of five SF-derived clones tested expressed either V beta 5.2 or a closely related beta chain, V beta 5.6. The alpha chains expressed by V beta 5.2+ and V beta 5.6+ clones were from different families, V alpha 2.4 and V alpha 23.2, respectively. Nine of ten clones derived from two cloning procedures on PB taken 3 years later also expressed either V beta 5.2 or V beta 5.6. This suggests that the TcR repertoire for recognizing this major histocompatibility complex/peptide complex is relatively restricted and favors the use of V beta 5. Conservation of the beta chain third complementarity-determining region (CDR3) sequence was not evident, however. Sequencing alpha and beta chains of representative V beta 5.2+ and V beta 5.6+ PB-derived clones revealed TcR which were identical to those utilized by the SF-derived clones, showing that the repertoire for recognition of this antigen is stable over time. Similar studies of TcR expression were carried out on hsp65-specific, DP4-restricted clones derived from the SF of a patient with rheumatoid arthritis by two independent cloning procedures. There was conservation of alpha chain usage, since all clones expressed a member of the V alpha 1 family, but again CDR3 sequence conservation was not apparent. beta chain usage was not restricted since different clones expressed V beta 6.7, V beta 22.3 and V beta 12. Subtle differences in epitope specificity were detected for two clones with differing TcR. Once more, T cell clones with identical alpha and beta TcR chains were obtained from the separate cloning procedures, suggesting oligoclonalty of T cells with this defined specificity in the patient's SF.

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Studies on the human T cell receptor α/β variable region genes. I. Identification of 7 additional V_α subfamilies and 14 J_α gene segments

Cited by 116 publications

References 35 publications

The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.

The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.

Characterization of the human T cell receptor α‐chain repertoire and demonstration of a genetic influence on Vα usage

Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65‐kDa heat‐shock protein: Selective in vivo expansion of T cells bearing defined receptors

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Studies on the human T cell receptor α/β variable region genes. I. Identification of 7 additional Vα subfamilies and 14 Jα gene segments

Cited by 116 publications

References 35 publications

The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.

The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.

Characterization of the human T cell receptor α‐chain repertoire and demonstration of a genetic influence on Vα usage

Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65‐kDa heat‐shock protein: Selective in vivo expansion of T cells bearing defined receptors

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Studies on the human T cell receptor α/β variable region genes. I. Identification of 7 additional V_α subfamilies and 14 J_α gene segments