Studies on the human T cell receptor α/β variable region genes. II. Identification of four additional V<sub>β</sub> subfamilies

Ferradini, Laurent; Roman‐Roman, Sergio; Azócar, José; Michalaki, H.; Triebel, Frédéric; Hercend, Thierry

doi:10.1002/eji.1830210412

Cited by 144 publications

(68 citation statements)

References 30 publications

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“…In this regard, it is of interest that expression of Vp21, which was also found to be increased in SF, has -70% homology with $6 (35). Although the degree of homology is not enough to group $6 and V, 21 together as a family, it suggests that both may recognize related antigens.…”

Section: Discussionmentioning

confidence: 99%

Increased usage of v_β2 and v_β6 in rheumatoid synovial fluid t cells

Cooper

Roessner

Naito-Hoopes

et al. 1994

Arthritis & Rheumatism

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Objective. To determine if the T cell antigen receptor V, usage of unstimulated rheumatoid arthritis (RA) synovial fluid (SF) T cells is biased compared with those in peripheral blood (PB).Methods. Freshly isolated, matched synovial fluid and peripheral blood T cells were analyzed for V, gene expression using quantitative polymerase chain reaction (PCR) methods. Ten synovial fluid samples from the knees of 7 patients with RA were studied. The PCR assay used 26 V , primers with a constant region C, primer, and 2 C, primers that co-amplied a product that served as an internal standard. Cycle number and complementary DNA content were controlled to ensure the linear accumulation of PCR products. Labeled products were separated on 10% polyacrylamide gels and counted with a Betascope blot analyzer.Results. There were consistent differences between the V, gene usage of SF and PB T cells directly isolated from patients with RA, regardless of HLA-DR haplotype. In all synovial specimens, V, 2 was increased relative to the peripheral blood, while Vj13.1 and V,13.2 were decreased. V& and b 2 1 were increased in 9 of the 10 synovial samples. Analyses of bilateral SF specimens from 2 subjects and serial specimens from the same knee of 1 subject revealed virtually identical patterns in each Submitted for publication April 19, 1994; accepted in revised form June 28, 1994. patient. The SF V, bias was not solely due to differences in the proportion of CD4+ and CD8+ cells, because the CD4:CD8 ratios in SF and PB were similar. However, V , gene usage of separated CD4+ and CD8+ synovial T cells showed that V $ and V ' were more highly expressed on CD4 cells.Conclusion. Freshly isolated synovial T cells from inflamed (not end-stage) knees of patients with RA have a remarkably consistent biased V, gene usage compared with PB T cells. V, 2 and V, 6 are uniformly increased, and this increase is primarily in CD4+ T cells. The same V, bias in the SF T cells of several RA patients suggests that shared antigens may be stimulating the T cell response.

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Section: Discussionmentioning

confidence: 99%

Increased usage of v_β2 and v_β6 in rheumatoid synovial fluid t cells

Cooper

Roessner

Naito-Hoopes

et al. 1994

Arthritis & Rheumatism

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“…Nucleotide sequences of the recombinant clones were performed using an Applied Biosystems model 377 sequencer following manufacturer's suggested protocols. For the V,B genes, sequences were aligned to the published sequences Vf317.1 (23) and V,316.1 (24).…”

Section: Methodsmentioning

confidence: 99%

Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations.

Mingari

Schiavetti

Ponte

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

223

185

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A small percentage of human T lymphocytes, predominantly CD8+ T cells, express receptors for HLA class I molecules of natural killer type (NK-R) that are inhibitory for T-cell antigen receptor (TCR)-mediated functions. In the present study, it is demonstrated that the various NK-R molecules typically expressed by NK cells are also expressed on periheral blood T lymphocytes. These CD3+ NK-R+ cells have a cell surface phenotype typical of memory cells as indicated by the expression of CD45RO and CD29 and by the lack of CD28 and CD45RA. Furthermore, by the combined use of anti-TCR Vf3-specific antibodies and a semiquantitative polymerase chain reaction assay, the TCR repertoire in this CD3+ NK-R+ cell subset was found to be skewed; in fact, one or two V8 families were largely represented, and most of the other Vj8s were barely detected. In addition, analysis of recombinant clones of the largely represented V,B families demonstrated that these Vf3s were oligoclonally or monoclonally expanded.

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“…TCR primer sequences were as in Reference (17). Samples were amplified by PCR for 30 cycles (30 s at 94°C, 30 s at 64°C, and 30 s at 72°C) for both TCR V␤ and TCR V␣ primers.…”

Section: Pcr Amplification and Sequencingmentioning

confidence: 99%

HLA-DP Allele-Specific T Cell Responses to Beryllium Account for DP-Associated Susceptibility to Chronic Beryllium Disease

Lombardi

Germain

Uren

et al. 2001

The Journal of Immunology

104

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Occupational exposure to small molecules, such as metals, is frequently associated with hypersensitivity reactions. Chronic beryllium (Be) disease (CBD) is a multisystem granulomatous disease that primarily affects the lung, and occurs in ∼3% of individuals exposed to this element. Immunogenetic studies have demonstrated a strong association between CBD and possession of alleles of HLA-DP containing glutamic acid (Glu) at position 69 in the HLA-DPβ-chain. T cell clones were raised from three patients with CBD in whom exposure occurred 10 and 30 years previously. Of 25 Be-specific clones that were obtained, all were restricted by HLA-DP alleles with Glu at DPβ69. Furthermore, the proliferative responses of the clones were absolutely dependent upon DPβ Glu69 in that a single amino acid substitution at this position abolished the response. As befits a disease whose pathogenesis involves a delayed type hypersensitivity response, the large majority of Be-specific clones secreted IFN-γ (Th1) and little or no IL-4 (Th2) cytokines. This study provides insights into the molecular basis of DP2-associated susceptibility to CBD.

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Studies on the human T cell receptor α/β variable region genes. II. Identification of four additional V_β subfamilies

Cited by 144 publications

References 30 publications

Increased usage of v_β2 and v_β6 in rheumatoid synovial fluid t cells

Increased usage of v_β2 and v_β6 in rheumatoid synovial fluid t cells

Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations.

HLA-DP Allele-Specific T Cell Responses to Beryllium Account for DP-Associated Susceptibility to Chronic Beryllium Disease

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Studies on the human T cell receptor α/β variable region genes. II. Identification of four additional Vβ subfamilies

Cited by 144 publications

References 30 publications

Increased usage of vβ2 and vβ6 in rheumatoid synovial fluid t cells

Increased usage of vβ2 and vβ6 in rheumatoid synovial fluid t cells

Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations.

HLA-DP Allele-Specific T Cell Responses to Beryllium Account for DP-Associated Susceptibility to Chronic Beryllium Disease

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Studies on the human T cell receptor α/β variable region genes. II. Identification of four additional V_β subfamilies

Increased usage of v_β2 and v_β6 in rheumatoid synovial fluid t cells

Increased usage of v_β2 and v_β6 in rheumatoid synovial fluid t cells