“…This conclusion is supported by the appearance of the centrifuge diagrams, which show clearly that the concentration gradient falls to zero between the peaks, thereby implying independence of the flow of each component (Gilbert, 1959; Williams, Van Holde, Baldwin & Fujita, 1958). This behaviour may be compared with that of such systems as ac-chymotrypsin (Massey, Harrington & Hartley, 1955) and P-lactoglobulin (Ogston & Tilley, 1955), where rapid equilibration occurs between monomer and polymers. In respect of (b), the mechanism of polymerization, intermolecular disulphide bridges could arise either by direct oxidation of sulphydryl groups on separate molecules or by the mercaptan-disulphide interchange, originally proposed by Huggins et al (1951) to account for the gelation phenomena encountered with plasma albumin in urea solutions.…”