Studies on the Hamster Papilloma and the Hamster Virus Lymphoma

Graffi, A; Bender, E; Schramm, Thomas; Graffi, I; Bierwolf, D

doi:10.1159/000391720

Cited by 21 publications

(23 citation statements)

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“…wt. of -3.1 x 106 have been isolated from homogeneous preparations of virus obtained from the primary skin epithelioma (Graffi et al, 1970). A preliminary characterization of this DNA has established that distinct homologies exist with polyoma and to a lesser extent with SV40 genomes (Scherneck et al, 1984), despite totally divergent restriction maps.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, both the virus and its DNA are capable of inducing lymphomas and leukemias when inoculated subcutaneously into newborn hamsters; the incidence is high (30-80 %) and the latency short (4-8 weeks). Thymectomized animals are poorly susceptible to these induced diseases suggesting that T lymphocytes may be the target of infection (Graffi et al, 1970). Virions have not been detected in the lymphoma or leukemia cells.…”

Section: Introductionmentioning

confidence: 99%

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A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

Delmas¹,

Bastien²,

Scherneck³

et al. 1985

The EMBO Journal

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The hamster papovavirus (HapV) is associated with multiple skin epitheliomas of the Syrian hamster. We have sequenced its genome. It is a double-stranded circular DNA of 5366 bp. The hypothetical genomic organization deduced from this nucleotide sequence is clearly of the polyoma type with the two strands coding in the opposite directions from a noncoding region that shows some of the features of a replication origin and a transcription control region. The amino acid sequences predicted from the open reading frames show an average of 50% homology with polyoma-coded polypeptides.The HapV is, after polyoma, the second example of a papovavirus coding for a middle T antigen. The cloned DNA can immortalize primary rat embryo cells and transform an established rat cell line. The viral DNA is stably integrated into the host genome.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

Delmas¹,

Bastien²,

Scherneck³

et al. 1985

The EMBO Journal

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confidence: 84%

Cell-free transmissible leukoses in Syrian hamsters, probably of viral aetiology

Graffi¹,

Schramm²,

Bender³

et al. 1968

Br J Cancer

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IN an earlier communication we reported on multiple epithelial skin tumours in Syrian hamsters in which a Papova virus has been detected with great regularity, in large quantities, and in characteristic histological distribution, as evidenced by electron microscopic observation (Graffi et al., 1967). In experiments designed to transmit this disease by means of subcellular extracts from these tumours to other animals we surprisingly obtained in Syrian hamsters and, in certain circumstances, also in rats, leukoses and reticuloses. It is these that are the subject of the present report. MATERIALS AND METHODSSubcellular tumour extracts were prepared as follows: tissue from skin tumours of hamsters ( Fig. 1 and 2) was vigorously homogenized in a glass homogenizer in phosphate-buffered saline (PBS) solution and the homogenate was diluted 1: 5 to 1: 10 and centrifuged. One group of animals was treated with the supernatant of the homogenate obtained after centrifugation at 3000 r.p.m. for 20 minutes. In most experiments, however, the material injected was subjected to centrifugation at 4000 to 6000 r.p.m. twice or three times, followed by filtration through G4 glass filters twice. In some cases the filtrate was diluted 1: 1 with 0 * 25 M saccharose solution and centrifuged at 100,000 g for 40 to 60 minutes and the sediment was administered to the animals after suspension in PBS solution. By the same series of procedures, cell-free G4 filtrates were obtained from hamster leukoses induced by the injection of papilloma-derived material and from cell transplants of such leukoses. All manipulations were carried out in the cold (2°C.) as quickly as possible. The extracts were administered to (1) newborn Syrian hamsters of our own random-bred hamster colony that has a low spontaneous incidence of the type of skin tumour in question (maximum 5 per cent) and a low incidence of leukaemias mainly lymphomas (Horn and Siewert, 1968) arising in the mesenteric lymph nodes (about 3 per cent); (2) newborn Syrian hamsters of another hamster colony in which neither of the two types of tumour occur spontaneously; (3) newborn rats of a Wistar strain that has a spontaneous leukaemia incidence of about 0 5 per cent. The filtrate was in most cases injected subcutaneously, but occasionally intraperitoneally, in doses of 0 * 2 to 0 * 3 ml. per newborn hamster, or 0 -4 to 0 * 5 ml. per newborn rat.The electron microscopic investigation was performed with ultra-thin sections from tumours and from leukaemic infiltrates in liver, lymph nodes, spleen, kidney, etc. Material was fixed in glutaraldehyde and osmic acid and embedded in Epon. Uranyl and lead acetate were used to make contrast preparations.

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“…Virus-induced tumours of stratified squamous-cell epithelium, such as plantar warts in man and papillomas in Syrian hamsters (Graffi et al, 1970) are benign and tend to regress. Perhaps more comparable, as the oral mucosa is involved, is multiple papillomatosis of the tongue in rabbits, but again these tumours are benign and always regress (Weisbroth, 1974).…”

Section: Pathoyenesismentioning

confidence: 99%

An inbred strain of rats with a high incidence of squamous-cell carcinomas of the mouth

Buckley¹,

Hulse²,

Keep³

1980

Br J Cancer

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Summary.-Intra-oral squamous-cell carcinomas occurred in over 50O0 of the HMT inbred strain of rats. In the outbred stock from which it was derived the incidence was 5O% or less, both when inbreeding was begun and after the inbred strain was fully established. Various factors in food and husbandry which might have irritated the oral mucosa were investigated, but there was no significant evidence that they played any part in the high incidence of mouth tumours.It is concluded that there must have been an accidental selection during inbreeding in favour of rats which had an inherited tendency to develop squamous -cell carcinoma of the mouth.There are a number of similarities between the rat tumour and intra-oral squamous-cell carcinoma in man and it is suggested that the rat tumour could be used as a model of the human disease.A BREEDING NUCLEUS of the "Alderley Park (Strain 1)" SPF outbred albino rats (Paget & Lemon, 1965) Leonard, personal communication, 1974).The first evidence of a high incidence of squamous-cell carcinoma of the mouth was in rats of the 12th-l 5th generation of inbreeding, which came froml anl experiment involving localized irradiation of the testes (Hulse, 1977). These rats were born 1970-1971, and during the latter part of 1973 a surprisingly large number developed intra-oral carcinoma of the mouth. Only males were observed at that time, and many had been irradiated, albeit well away from the mouth, so a formal investigation was necessary to find the exact incidence in both sexes. A number of factors in the rats' environment which might possibly have accentuated any tendency to develop mouth tumours were also examined. METHOD OF iNVESTIGATIONThe rats' cages, their food and their driniking water were thought to be possible sources of irritants to their oral mucosa. The incidence of mouth tumours wxas also studied in a group of rats imported from the parent stock.Cages.-The galvanized-wire cages which wxve w%vere using were old. and, as the galvanizing was inclined to come off, it was conceiv-* Pr'eseint adclress: H imitingdon Researcht Centre, Ht1itltiIg(olon, Cambri(dgesli1il(r.Requiests for reprints: Dr E. V. Hulse.

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Studies on the Hamster Papilloma and the Hamster Virus Lymphoma

Cited by 21 publications

References 0 publications

A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

Cell-free transmissible leukoses in Syrian hamsters, probably of viral aetiology

An inbred strain of rats with a high incidence of squamous-cell carcinomas of the mouth

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