Studies on the glycolipids of sheep thymus and of normal and concanavalin A-stimulated sheep peripheral lymphocytes

Narasimhan, R; Hay, J. B.; Greaves, Melvyn F.; Murray, Robert K.

doi:10.1016/0005-2760(76)90222-8

Cited by 31 publications

(13 citation statements)

References 32 publications

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“…It is known that surface-binding agents regulate the division of animal cells by altering the composition of cell surface glycolipids (12,23). However, reports are not available pertaining to the effect of antibodies to lipid antigens on grampositive microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Influence of antibodies to mannophosphoinositides on phospholipid synthesis in Mycobacterium smegmatis ATCC 607

Penumarti¹,

Khuller²

1982

Infect Immun

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Antiserum to mannophosphoinositides had a significant inhibitory effect on phospholipid synthesis in Mycobacterium smegmatis ATCC 607 at different phases of growth; however, the inhibition was more pronounced in the exponential phase. The rate of synthesis of total phospholipids and individual phospholipids, viz., cardiolipin, phosphatidylethanolamine, and mannophosphoinositides, decreased in the presence of antiserum as compared with normal serum. The maximum inhibitory effect of antiserum was exerted on the synthesis of mannophosphoinositides, particularly on tetraacylated dimannophosphoinositide. Antiserum also had a growth inhibitory effect as compared with normal serum.

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Section: Discussionmentioning

confidence: 99%

Influence of antibodies to mannophosphoinositides on phospholipid synthesis in Mycobacterium smegmatis ATCC 607

Penumarti¹,

Khuller²

1982

Infect Immun

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“…Extraction of lipids was performed by the method of Suzuki (1965). The upper and lower phases of the extracts obtained by this procedure were subjected to methanolysis in mild alkali as described previously (Narasimhan et al, 1976); preliminary experiments revealed that none of the glycolipids of the lung and tumours studied were affected by this treatment. The lipids of the lower and upper phases were then fractionated by silicic acid column chromatography (Vance & Sweeley, 1967;Yogeeswaran et al, 1972).…”

Section: Isolation Of Neutral Glycosphingolipid and Ganglioside Fractmentioning

confidence: 99%

“…Samples of the neutral glycosphingolipid and ganglioside fractions were subjected to t.l.c. on glass plates coated with silica gel G (250pm thickness; Merck A.G., Darmstadt, West Germany) as described previously (Narasimhan et al, 1976); the standard solvent systems were chloroform/methanol/conc. NH3/water (65:35:1:7, by vol.)…”

Section: Isolation Of Neutral Glycosphingolipid and Ganglioside Fractmentioning

confidence: 99%

Neutral glycosphingolipids and gangliosides of human lung and lung tumours

Narasimhan¹,

Murray²

1979

Biochemical Journal

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In order to help determine whether alterations of the profiles of glycosphingolipids occur consistently in human tumours, the neutral glycosphingolipids and gangliosides of nine lung tumours (one adenocarcinoma, four squamous cell, two mixed adeno-squamous cell, one large cell and one oat-cell carcinomata) were analysed. The control tissue consisted of adjacent lung; it contained neutral glycosphingolipids corresponding in properties to glucosyl-, lactosyl-, globotriaosyl- and globotetraosyl-ceramides. All of the tumours also contained these four neutral glycosphingolipids. However, in addition, five of the tumours (two of the squamous, the large cell and the two mixed adeno-squamous cell carcinomata) contained neutral glycosphingolipids corresponding in properties to lactotriaosyl- and neolactotetraosyl-ceramides; these same tumours also exhibited higher amounts of lactosylceramide than the other tumours analysed. Both of the two former neutral glycosphingolipids and very substantial amounts of the latter neutral glycosphingolipid were detected in pneumonic lung and in polymorphonuclear leucocytes; it thus appears possible that these particular compounds were derived from these latter cells rather than from the tumour cells. The ganglioside patterns of the tumours were almost equivalent in complexity to that exhibited by the control lung tissue. This study shows that the profiles of two major classes of glycosphingolipids (neutral glycosphingolipids and gangliosides) occurring in lung tumours are almost as complex as those of the parent tissue, a finding in contrast with the notably simplified patterns of these lipids found in many cancer cells grown in vitro. It also suggests that when lactotriaosyl- and neolactotetraosyl-ceramides and high amounts of lactosylceramide are detected in human tumours, the possibility must be considered that these compounds are derived from polymorphonuclear leucocytes.

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“…3. Rat and human thymus, like sheep thymus [Narasimhan, Hay, Greaves & Murray (1976) Biochim. Biophys.…”

mentioning

confidence: 99%

Comparative study of the glycosphingolipids of chicken bursa of Fabricius and of chicken, rat and human thymus

Narasimhan¹,

Murray²

1978

Biochemical Journal

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1. The glycosphingolipid compositions of the thymus and bursa of Fabricius of young male chickens were compared. The two tissues were found to contain complex mixtures of both neutral glycosphingolipids and gangliosides. Both tissues contained mono-, di-, tri-, tetra- and penta-glycosylceramides; the pentaglycosylceramide displayed a reaction of identity with authentic Forssman antigen when tested against a specific anti-(Forssman antigen) serum. The ganglioside G(m3) containing N-acetylneuraminic acid was the principle ganglioside of both tissues. 2. The thymus contained appreciable amounts of the simple ganglioside N-acetylneuraminylgalactosylceramide, a compound not found in the bursa. The ganglioside G(d3) (disialohaematoside) was detected in both tissues. 3. Rat and human thymus, like sheep thymus (Narasimhan, Hay, Greaves & Murray (1976) Biochim, Biophys. Acta 431, 578-591), both contained a tetraglycosylceramide species as their most complex neutral glycosphingolipid and possessed little or no Forssman antigen. They also contained a complex mixture of gangliosides. 4. The possible significance of these results is briefly discussed.

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Studies on the glycolipids of sheep thymus and of normal and concanavalin A-stimulated sheep peripheral lymphocytes

Cited by 31 publications

References 32 publications

Influence of antibodies to mannophosphoinositides on phospholipid synthesis in Mycobacterium smegmatis ATCC 607

Influence of antibodies to mannophosphoinositides on phospholipid synthesis in Mycobacterium smegmatis ATCC 607

Neutral glycosphingolipids and gangliosides of human lung and lung tumours

Comparative study of the glycosphingolipids of chicken bursa of Fabricius and of chicken, rat and human thymus

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