Studies on the Etiology of Marek's Disease. I. Propagation of the Agent in Cell Culture

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

“…1B). To construct transfer plasmids for generating rMDV1s, pKA4L(F) (47), which contains NDV-F cDNA (52) under the control of the SV40 late promoter, was used. A HindIII-XhoI fragment of pKA4L(F) was substituted for the F, P, or N fragment, and the resulting plasmids were designated pKA4F(F), pKA4P(F), and pKA4N(F), respectively.…”

“…Marek's disease virus (MDV) is an etiological agent of Marek's disease (MD), a highly contagious malignant T-lymphomatosis of chickens caused by MDV serotype 1 (MDV1) (10,32,52). MD represents the first cancer to be prevented and controlled by the use of live attenuated or naturally avirulent vaccines (11,12).…”

The Attenuation, with Loss of Oncogenicity, of the Herpes-type Virus of Marek's Disease (Strain hprs-16) on Passage in Cell Culture

“…Previous investigations have provided strong evidence that a herpes-type virus is the cause of Marek's disease (Churchill & Biggs, 1967;Solomon et al 1968;Nazerian et al 1968;Churchill & Biggs, 1968;Biggs et al i969).…”

Demonstration of immunoglobulin m on avian lymphoid leukosis lymphoma cells by the unlabelled antibody peroxidase‐antiperoxidase method

“…This has been evidenced by demonstrating the presence of both immunoglobulin M (IgM) and B-cell antigenic markers on the surface of virtually all tumour cells (Cooper et al, 191'4;Payne and Rennie, 1975). In contrast, MD is essentially a malignancy of the thymic (T) system of lymphocytes, caused by a herpesvirus (Churchill and Biggs, 1967;Nazerian et al, 1968;Solomon et al, 1968). MD lymphomas consist mainly of T-cells with only a small population of B-cells (Hudson and Payne, 1973;Rouse et al, 1973;Payne et al, 1974;Payne and Rennie, 1976).…”