Studies on the Entero-Hepatic Circulation of 14c Porphyrins

Stathers, George M.; Aronsen, K. F.; Hæger-Aronsen, Birgitta

doi:10.1111/j.1365-2133.1969.tb13980.x

Cited by 3 publications

(1 citation statement)

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“…In the literature the enterohepatic circulation of porphyrins constitutes a controversial issue. Some reports state that such a circulation exists (11)(12)(13), whereas others state the reverse (14). It might be that secondary porphyrins are present in bile or serum in trace amounts.…”

Section: Discussionmentioning

confidence: 96%

Determination of Porphyrins in Bile Using High Performance Liquid Chromatography and Some Clinical Applications

Meerman²,

Huizenga³

et al. 1994

CCLM

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A simple and fast HPLC method for the determination of ροφΗγπηβ in bile without extraction is described. ΡθφΗγΗη8 were determined in bile from control subjects and from patients after orthotopic liver transplantation, ineluding three patients with eiythropoietic protoporphyria. It was found that: 1) coproporphyrin I is the predominant porphyrin in bile of controls, accompanied by some coproporphyrin III and protoporphyrin, whereas protoporphyrin mostly but not always is the predominant porphyrin in the bile of erythropoietic protoporphyria patients. In two of the three erythropoietic protoporphyria patients, the bile contained a hundred times more protoporphyrin than that of ήοη-porphyric orthotopic liver transplantation patients. The third erythropoietic protpporphyria patient remained cholestatic and was unable to excrete sufficient amounts of protoporphyrin.2) All investigated bile samples contained no secondary porphyrins derived from protoporphyrin, i. e. no deutero-, pempto-, or mesoporphyrin. Even when extracts of bile and serum were concentrated fifty to a hundred times, no traces of deutero^, pempto-and mesoporphyrin were detected. This complete absence of secondary porphyrins suggests that an enterohepatic circulation of dicarb xylic porphyrins from the distal gastrointestinal tract does not exist.3) The HPLC chromatqgfarns contain peaks from unknown compounds. No correlation between ροφ!^τίη8 and these compounds was found.Porphyrin profiles were followed in the bile of some orthotopic liver transplantation patients. Three episodes are recognizable. D ring the first three days after orthotopic liver transplantation there is a very high οορΓοροφΙ^ιίη excretion. There is then a lag of one to three weeks, in which no or very low ρoφhyrin concentrations are detectable, followed by the restoration of normal biliary ροφίιγιίη patterns. IntroductionThe poφhyric diseases can be identified by profiling of urine, faeces or blood (1-3), and detecting the ροφίν/-Determination of ροφίι^η profiles in biological sain-rins that are ffQ^y elevated. Thus, together with their plesbyrneansofHPLChasprovedvaluableinthediag-clinical presentation, congenital erythropoietic pornosis and treatment of patiemts suffering from currently phyria, poφhyria cutanea tarda and attacks of acute inknownforms ofpoφhyria, allcausedby adeficiency or termittent poφhyria can be identified by analysis of Inhibition of enzymes involved in the synthesis of haem. urine, whereas stool analysis is needed to identify at-

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