Studies on the efficiency of different extraction procedures on the anti microbial activity of selected medicinal plants

Abstract

Many doubts still persist even today when it comes to selection of the solvents for extracting the active constituents from various Indian medicinal plants. This study was aimed at assessing and establishing the best solvent for extracting the active constituents from 10 plant extracts. Thin layer chromatography (TLC) was used to separate and establish the active constituents present in each of the medicinal plants. Active constituents from each plant were extracted by using three different solvent systems namely diethyl ether, chloroform and hexane and were tested against three species of gram negative and three species of gram positive bacteria (Escherichia coli, Pseudomonas aureginosa, Streptococcus pneumoniae, Aeromonas hydrophila, Staphylococcus aereus, Bacillus cereus) by means of agar well diffusion assay. Studies on the antioxidant activity studies were also carried out for these plant extracts by using Diphenylpicryl-hydrazyl (DPPH) method. For the antimicrobial activity, the study revealed that among the selected plants, Azadiracta indica, Pongamia pinnata, Aloe barbadensis had the maximum antibacterial activity. Among the extraction procedures diethyl ether was found to be the best solvent that could be used for the extraction procedure. On the antioxidant activity part, Coleus amboinicus and Calotropis procera were found to have high antioxidant activity of 91.64% and 88.72% respectively and the further results are reported and discussed.

Antibacterial Activity of Extracts ofMyrtus communisAgainst Food-Borne Pathogenic and Spoilage Bacteria

“…[14] To the best of the authors' knowledge and following a survey of the pertinent literature, only a few reports about the antibacterial activity of the essential oils and crude methanol extract of M. communis leaves have been published [15][16][17] , although several authors have found that the solvent used and the extraction system may modify the antibacterial activity of the extracts. [18] For these reasons, the aim of the work was to test the efficacy of various leaf and berry extracts against a diverse range of food-borne pathogens and food spoilage bacteria.…”

Evaluation of Metabolite Profiles of Ginseng Berry Pomace Obtained after Different Pressure Treatments and Their Correlation with the Antioxidant Activity

“…Moreover, ethyl acetate is inexpensive, which is good from an economical viewpoint [ 41 ]. Chloroform is non-inflammable and is used for extraction of the active constituents from shrubs and other related plant species [ 42 ].…”

Effect of Preparation and Extraction Parameters of Banana (Musa balbisiana cv. Saba) Inflorescence on their Antibacterial Activities

“…Non-polar organic solvent extractions such as hexane and petroleum ether extracts show very low antibacterial activity (6.47 to 10.64 mm) on Staphylococcus aureus (SA) and Bacillus cereus (BC). Muthuvelan and Raja (2008) suggested that the polarity distribution of antibacterial compounds in plants varies depending on species and plant families. Non-polar solvents may not be a good choice in extracting antibacterial metabolites in this case since most of the targeted metabolites are at the moderately polar end of the spectrum.…”