Studies on the effects of mutagens on cytotoxicity behaviour in Pigeon pea (Cajanus cajan (L.) Millsp) Var.CO-7.

Abstract

The cytological studies provide more information regarding the response of a genotype to the particular mutagen and also provide chances to select desirable characters. The seeds of pigeon pea were subjected to different doses and concentrations of gamma rays and ethyl methane sulphonate (EMS). The effects of different mutagenic treatments on mitosis chromosomal behavior have been studied in both the mutagens. Different types of mitotic aberrations like stickiness, precocious movement, bridge, clumping of chromosome and laggards, etc., were observed in all the treatments. However, the gamma rays treatments proved to be more effective in inducing mitotic aberrations as compared to EMS. The frequency of laggard was high when compared to other mitotic aberrations. The reduction in mitotic index and relative deviation rate frequency were observed with increase in doses and conc. of both the mutagenic treatments and gamma rays were found to be more effective than EMS treatments.

Germination and development of M1 seedlings of two Selliera radicans Cav. accessions subjected to gamma radiation

“…The stimulating effects of radiation on germination are still being studied. Some theories point to stimulating effects on enzymatic activity and on the synthesis of nucleic acids or the activation of protein synthesis; these effects occur during the initial stage of germination after irradiation of the seed, accelerating cell division and directly or indirectly stimulating auxin-response genes (7,35).…”

“…Determining the mean lethal dose (LD 50 ) is essential (54). The LD 50 value represents the amount of radiation or chemical agent absorbed with which 50% of the exposed population survives or that causes 50% growth reduction (7). Mutagens must be used in accordance with the aforementioned doses, since a greater (5) spectrum of mutations is generated; moreover, lower doses (less than 300 Gy) allow recovery of an adequate number of useful mutants compared to higher doses (29).…”

Revisiting the Nutritional, Chemical and Biological Potential of Cajanus cajan (L.) Millsp.

“…The cytological analysis of C. cajan showed that it is diploid having 2n = 2x = 22 chromosomes with an average length of 5.73 ± 1.15 µm up to 10.92 ± 2.69 µm and dominantly metacentric in shape, consisting of 14 metacentric and 4 submetacentric chromosomes [ 14 ]. C. cajan has a genome of size 858 mega-base pairs [ 15 ]. In the comparative genetic characterization of wild and cultivated C. cajan genotypes, the cultivated species present maximum polymorphic loci [ 6 ].…”

Особливості Впливу Епімутагену Тритон-305х На Рослини Пшениці Озимої

“…Генетичне поліпшення пшениці озимої як постійний процес обумовлено потребами у зростанні продуктивності та якості зерна пшениці озимої, ураховуючи що даний злак посідає постійне місце серед трьох лідуючих злакових культур за площами та обсягами виробництва разом з кукурудзою та рисом [9,10]. Генетичне поліпшення через використання мутаційних змін дозволяє в доволі обмежені строки створити принципово нові форми, котрі є носіями унікальних, або обмежено-поширених через погану рекомбінацію ознак у цієї культури [2,3].…”