Studies on the effect of phyllanthus emblica extract on the growth of urinary type struvite crystals invitro

Bindhu, B.; Swetha, A. S.; Veluraja, K.

doi:10.1186/s40816-015-0004-1

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…Such changes in the structure of the struvite crystals were also reported earlier (22, 23). Vitamin C interferes in the crystal growth and the preferred growth being at (111) plane and the XRD pattern of struvite crystals grown in the gel medium are identical with the reported literature (7, 24). The struvite crystals exhibited porous nature and the multi-layered depositions.…”

Section: Discussionsupporting

confidence: 87%

“…The struvite crystals exhibited porous nature and the multi-layered depositions. These multi-layered depositions are commonly observed during the formation of struvite crystals in vitro in the presence of natural compounds (24). The internal porous nature with characteristic tubular pores of struvite crystal during P. mirabilis induced crystallization was well explained (8).…”

Section: Discussionmentioning

confidence: 99%

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Vitamin C inhibits crystallization of struvite from artificial urine in the presence of Pseudomonas aeruginosa

et al. 2018

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Background: Formation of struvite stones is associated with urinary tract infection by urease-producing bacteria. Biogenic crystal growth in natural and synthetic materials is regulated by the action of inhibitors, ranging from small ions, molecules to large macromolecules. Materials and Methods: We report the dynamics of in vitro crystallization of struvite in presence of vitamin C in synthetic urine using single diffusion gel growth technique. Sodium metasilicate gel of specific gravity 1.05 and the aqueous solution of ammonium dihydrogen phosphate were used as the medium for growing the struvite crystals. The crystallization process was induced by a urease positive struvite stone associated Pseudomonas aeruginosa to mimic the infection leading to stone formation. The grown crystals were characterized by ATR-FTIR and powder XRD. The surface morphology was analysed through FE-SEM for comparison between treatments. Results: We observed decrease in number, dimension, and growth rate of struvite crystals with the increasing concentrations of vitamin C. Crystals displayed well-defined faces and dendritic morphology of struvite in both control and biogenic systems. Conclusion: The results strongly suggest that, vitamin C can modulate the formation of struvite crystals in the presence of uropathogenic bacteria.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Vitamin C inhibits crystallization of struvite from artificial urine in the presence of Pseudomonas aeruginosa

et al. 2018

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“…The experimental value of bandgap energy for struvite of 5.07 eV was also reported by Bindhu et al Unfortunately, the authors did not provide information on the procedure they employed to obtain such a value, which makes it difficult to explain a deviation amounting to about 0.9 eV from bandgap values shown in Table .…”

Section: Experimental Determination Of Struvite Optical Bandgapmentioning

confidence: 71%

Structural and Optical Properties of Struvite. Elucidating Structure of Infrared Spectrum in High Frequency Range

et al. 2020

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Study of structure and optical properties of magnesium ammonium phosphate hexahydrate crystal known as struvite is presented. Experimentally determined infrared (IR) and ultraviolet–visible (UV–vis) spectra are compared with the theoretical predictions of density functional methods. Examination of the interatomic bond lengths, Mulliken atomic charges, and binding energies of water in the magnesium hexahydrate cation, together with the analysis of the hydrogen bond pattern have allowed us to explain a special feature of the IR spectrum of struvite, a blueshift of the band corresponding to the O–H stretching mode. This mode has been assigned to a “dangling” hydroxyl group in one of the water molecules in magnesium hexahydrate. Using experimentally obtained UV–vis spectrum and performing Tauc plots analysis, optical bandgap of struvite has been narrowed to a range from 5.92 to 6.06 eV.

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“…Usually, the urinary stones are crystal components such as brushite [CaHPO 4 .2H 2 O], struvite [(NH 4 )MgPO 4 - 6(H 2 O)], calcium oxalate [Ca(COO) 2 ], hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 ], etc. Among phosphates, struvite stone made of magnesium ammonium phosphate hexahydrate (MAPH; MgNH 4 PO 4 ·6H 2 O) accounts 10–20% consider as the second predominant crystalline component which is rapidly growing and lifetime frightening disorder, especially in women 14, 15. Bacterial infection may stimulate struvite stone development by crystal adherence and are caused by the action of bacteria on urine 16 due to the alkalization of urine by the urease producing bacteria such as Proteus species, Klebsiella , Pseudomonas and Corynebacterium species also by E. coli 17 …”

Section: Introductionmentioning

confidence: 99%

Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone)

Kaleeswaran¹,

Ramadevi²,

Murugesan³

et al. 2019

Journal of Traditional and Complementary Medicine

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Pedalium murex (L.) is a traditional herb, commonly used for the treatment of kidney stone related problems. Struvite stone can swiftly grow and become ‘staghorn calculi’ in kidney and its associated areas, which is the most aching urological disorder. The present study investigated the anti-urolithiasis activities of ethyl acetate extract of P. murex L. (EAEP) against struvite crystal. The antibacterial activity of EAEP examined against several urease producing bacteria. It showed the minimum bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus (>125). On the other hand, total mass, volume, number, growth rate and dissolution rate of synthesised struvite crystals were observed at different concentrations 0.5%, 0.75%, 1% of EAEP and without EAEP. In which, EAEP addition showed appreciably reduced struvite crystal. Alternatively, MgO (300 mg of EAEP/kg/body weight) induced urolithiasis of Wistar albino rat at the rate of 1 ml for 28 days. Various biochemical parameters in serum, urine and histological analysis of kidney were taken for evaluation. Significant results (p < 0.05) were observed in 1% EAEP (300 mg) treated group than cystone treated group. From the histological study, reduced renal damage and glomerular development were observed. Our experiment, P. murex L. enhances the reducing activity on struvite crystal and prevents the crystal formation both in-vitro and in-vivo. It can be suggesting that P. murex L. and its phyto-components could be used as remedy for the management of kidney stone by dissolving the struvite stone in kidney.

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Studies on the effect of phyllanthus emblica extract on the growth of urinary type struvite crystals invitro

Cited by 7 publications

References 15 publications

Vitamin C inhibits crystallization of struvite from artificial urine in the presence of Pseudomonas aeruginosa

Vitamin C inhibits crystallization of struvite from artificial urine in the presence of Pseudomonas aeruginosa

Structural and Optical Properties of Struvite. Elucidating Structure of Infrared Spectrum in High Frequency Range

Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone)

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