Studies on the effect of toxin T-514 on the integrity of peroxisomes in methylotrophic yeasts

Saavedra, Julio Sepúlveda; Klei, Ida J. van der; Keizer, Ineke; Lopez, Alfredo PiÃ±eyro; Harder, Win; Veenhuis, Marten

doi:10.1111/j.1574-6968.1992.tb05210.x

Cited by 30 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also when peroxisomes are damaged (e.g. by chemical damage to either peroxisomal matrix compo-nents^using KCN [9]^or peroxisomal membrane com-ponents^using the toxin peroxysomicin A-1 [10])^peroxisomes are selectively removed from the cytoplasm.…”

Section: Peroxisome Homeostasis In H Polymorphamentioning

confidence: 99%

Peroxisome homeostasis in

Leão

Kiel

2003

FEMS Yeast Research

View full text Add to dashboard Cite

Peroxisomes are essential organelles in many eukaryotes. Until recently, the main focus of the investigations concerning these important organelles was to understand the biogenesis of the peroxisome (induction, proliferation and matrix protein import). However, when peroxisomes become redundant they are quickly degraded by highly selective processes known as pexophagy. The first molecular studies on pexophagy have indicated that this process shares many features with certain transport pathways to the vacuole (vacuolar protein sorting, autophagy, cytoplasm-to-vacuole targeting and endocytosis). Nevertheless, recent data demonstrate that in addition to common genes also unique genes are required for these transport processes. The main focus for the future should therefore be on identifying the unique determinants of pexophagy. Earlier results suggest that in the methylotrophic yeast Hansenula polymorpha proteins located on the peroxisome itself are required for pexophagy. Thus, it has become essential to study in detail the role of peroxisomal membrane proteins in the degradation process. This review highlights the main achievements of the last few years, with emphasis on H. polymorpha.

show abstract

Section: Peroxisome Homeostasis In H Polymorphamentioning

confidence: 99%

Peroxisome homeostasis in

Leão

Kiel

2003

FEMS Yeast Research

View full text Add to dashboard Cite

show abstract

“…It could be speculated as there is no information in the literature on this subject, that in those periods there are only immature peroxisomes, which would lead to the finding of micropexophagy reported here. In contrast, mature peroxisomes would be found at later incubation times, which would explain the observation of the macropexophagy reported in previous studies (Sepúlveda Saavedra et al, 1992; Salazar‐Aranda et al, 1998; Vargas‐Zapata et al, 1999). This hypothesis is compatible with the current model of peroxisome formation.…”

Section: Discussionmentioning

confidence: 66%

“…C. boidinii (American Type Culture Collection 32195) was pregrown in batch cultures in mineral media supplemented with 2% glucose at 30°C for 24 h. Afterward, the cells were shifted into 0.5% methanol‐containing media starting with suspensions of cells at an optical density of 0.1 at 600 nm. PA1 was added to the cultures at a final concentration of 2 µg/mL (Sepúlveda Saavedra et al, 1992) after they had reached the mid‐exponential growth phase. As negative controls, the cultures of untreated cells were used.…”

Section: Methodsmentioning

confidence: 99%

“…Sepúlveda Saavedra et al (1992) showed that T‐514 produces selective and irreversible damage of the peroxisomal membrane in vivo in the methylotrophic yeasts Hansenula polymorpha and Candida boidinii . For this reason, T‐514 was renamed as peroxisomicine A1 (PA1).…”

Section: Introductionmentioning

confidence: 99%

“…Sepúlveda Saavedra et al (1992), Salazar‐Aranda et al (1998), and Vargas‐Zapata et al (1999) found that in methylotrophic yeasts, peroxisomes damaged by PA1 are eliminated by macropexophagy. In those studies, PA1 was added to the yeast cultures after they had reached the mid‐exponential growth phase in methanol‐containing medium, and samples for analysis were taken between 30 min and several hours after the addition of PA1.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy

Ortega‐Martínez

Gutiérrez‐Dávila

Niderhauser‐García

et al. 2020

Cell Biology International

View full text Add to dashboard Cite

Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: macropexophagy and micropexophagy. Previous studies showed that peroxisomes damaged by a prolonged exposition to PA1 are eliminated by macropexophagy. In this work, Candida boidinii was grown in methanol‐containing media, and PA1 was added to the cultures at 2 µg/mL after they reached the mid‐exponential growth phase. Samples were taken at 5, 10, 15, 20, and 25 min after the addition of PA1 and processed for ultrastructural analysis. Typical morphological characteristics of micropexophagy were observed: the direct engulfment of peroxisomes by the vacuolar membrane and the presence of the micropexophagic membrane apparatus (MIPA), which mediates the fusion between the opposing tips of the vacuole to complete sequestration of peroxisomes from the cytosol. In conclusion, here we report that, in addition to macropexophagy, peroxisomes damaged by PA1 can be eliminated by micropexophagy. This information is useful to deepen the knowledge of the mechanism of action of PA1 and of that of pexophagy per se.

show abstract

Peroxisome biogenesis and degradation in yeast: A structure/function analysis

Veenhuis

Salomons

Klei

2000

Microsc. Res. Tech.

Self Cite

View full text Add to dashboard Cite

In yeast, peroxisomes are the site of specific catabolic pathways that characteristically include hydrogen peroxide producing oxidases and catalase. During the last 10 years, much progress has been made in unravelling the molecular mechanisms involved in the biogenesis of this organelle. At present, 23 different genes (PEX genes) have been identified that are involved in different aspects of peroxisome biogenesis (e.g., proliferation, formation of the peroxisomal membrane, import of matrix proteins). The principles of peroxisome degradation are still much less understood. Recently, the first yeast mutants affected in this process have become available and used to clone corresponding genes by functional complementation. In this paper, an overview is presented of the research on yeast peroxisomes, focusing on recent achievements in the molecular aspects of peroxisome development, function, and turnover. Microsc. Res. Tech. 51:584–600, 2000. © 2000 Wiley‐Liss, Inc.

show abstract

Studies on the effect of toxin T-514 on the integrity of peroxisomes in methylotrophic yeasts

Abstract: Harder, W., & Veenhuis, M. (1992). Studies on the effect of toxin T-514 on the integrity of peroxisomes in methylotrophic yeasts. FEMS Microbiology Letters, 91(3).

Cited by 30 publications

References 13 publications

Peroxisome homeostasis in

Peroxisome homeostasis in

Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy

Peroxisome biogenesis and degradation in yeast: A structure/function analysis

Contact Info

Product

Resources

About