Studies on the effect of AgNP binding on α-amylase structure of porcine pancreas and Bacillus subtilis by multi-spectroscopic methods

Ernest, Vinita; Sekar, Gajalakshmi; Mukherjee, Amitava; Chandrasekaran, N.

doi:10.1016/j.jlumin.2013.09.065

Cited by 13 publications

(9 citation statements)

References 39 publications

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“…Indeed, some studies underlined the ability of digestive enzymes to bind around NPs. [75][76][77][78][79][80] Besides, bile salts can also interact with the NPs and complete the corona. [41,81] This corona stabilizes the NPs as it was emphasized by the raise of negative zeta-potential.…”

Section: Discussionmentioning

confidence: 99%

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Laloux

Kastrati

Cambier

et al. 2020

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Silver nanoparticles (AgNPs) are used in the agri‐food sector, which can lead to their ingestion. Their interaction with food and their passage through the gastrointestinal tract can alter their properties and influence their fate upon ingestion. Therefore, this study aims at developing an in vitro method to follow the fate of AgNPs in the gastrointestinal tract. After incorporation of AgNPs into a standardized food matrix, a precolonic digestion is simulated and AgNPs are characterized by different techniques. The presence of food influences the AgNPs properties by forming a corona around nanoparticles. Even if the salivary step does not impact significantly the AgNPs, the pH decrease and the digestive enzymes induce the agglomeration of AgNPs during the gastric phase, while the addition of intestinal fluids disintegrates these clusters. AgNPs can thus reach the intestinal cells under nanometric form, although the presence of food and gastrointestinal fluids modifies their properties compared to pristine AgNPs. They can form a corona around the nanoparticles and act as colloidal stabilizer, which can impact the interaction of AgNPs with intestinal epithelium. This study demonstrates the importance of taking the fate of AgNPs in the gastrointestinal tract into account to perform an accurate risk assessment of nanomaterials.

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Section: Discussionmentioning

confidence: 99%

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Laloux

Kastrati

Cambier

et al. 2020

Small

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“…By monitoring the peaks corresponding to these structures, it was discovered that upon adsorption of BSA onto AgNPs and carbon‐based nanomaterials, with subsequent formation of a protein corona, β‐turns were relaxed, α‐helix decreased, and β‐sheet increased, possibly because the helical structure tends to unravel in order to interact with a particle surface . Structural changes in amylase were also confirmed upon interaction with AgNPs using IR spectra showing that the di‐sulfide bridges present in the native protein was lost due to the interaction . IR spectroscopy is also often used to confirm the surface modification or conjugation of nanomaterials, e.g., the disappearance of –SH stretching band from the free BSA molecule indicated successful conjugation of BSA–AuNPs through Au–thiol coordination bonds (Figure (c)); the interacting functional groups from the biopolymers, including chitosan, N,N,N‐trimethylchitosan (TMC), and carboxymethylchitosan (CMC), with the QD surfaces were identified when they were used as capping agents by the shifts and changes in intensities from their respective IR spectra …”

Section: Surface Interactions Of Nanomaterials—analytical and Physicomentioning

confidence: 99%

“…Circular dichroism (CD) detects the ellipticity caused by such polarization‐dependent absorption to provide structural information of the biomolecules . Thus, CD is widely used to study the structural changes of biomolecules such as proteins as they interact with biomaterial surfaces, as well as probing how corona proteins may be different from their native structures . Sophisticated deconvolution algorithms are typically required to calculated percentages of different secondary structures from the spectrum, and depending on the algorithm used, there can be some variations in the results.…”

Section: Surface Interactions Of Nanomaterials—analytical and Physicomentioning

confidence: 99%

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Chen

Riviere

2016

WIREs Nanomed Nanobiotechnol

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The understanding of nano-bio interactions is deemed essential in the design, application, and safe handling of nanomaterials. Proper characterization of the intrinsic physicochemical properties, including their size, surface charge, shape, and functionalization, is needed to consider the fate or impact of nanomaterials in biological and environmental systems. The characterizations of their interactions with surrounding chemical species are often hindered by the complexity of biological or environmental systems, and the drastically different surface physicochemical properties among a large population of nanomaterials. The complexity of these interactions is also due to the diverse ligands of different chemical properties present in most biomacromolecules, and multiple conformations they can assume at different conditions to minimize their conformational free energy. Often these interactions are collectively determined by multiple physical or chemical forces, including electrostatic forces, hydrogen bonding, and hydrophobic forces, and calls for multidimensional characterization strategies, both experimentally and computationally. Through these characterizations, the understanding of the roles surface physicochemical properties of nanomaterials and their surface interactions with biomacromolecules can play in their applications in biomedical and environmental fields can be obtained. To quantitatively decipher these physicochemical surface interactions, computational methods, including physical, statistical, and pharmacokinetic models, can be used for either analyses of large amounts of experimental characterization data, or theoretical prediction of the interactions, and consequent biological behavior in the body after administration. These computational methods include molecular dynamics simulation, structure-activity relationship models such as biological surface adsorption index, and physiologically-based pharmacokinetic models. WIREs Nanomed Nanobiotechnol 2017, 9:e1440. doi: 10.1002/wnan.1440 For further resources related to this article, please visit the WIREs website.

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“…It is due to the fluorescence of their constituent aromatic amino acid residues that enzymes exhibit such a property. Having numerous tryptophan (Trp) groups, α-amylase absorbs the light at the wavelength of 280 nm and re-emits it between 300 to 350 nm 44 . It has been observed that when an enzyme (which can act as a chromophore) binds onto an oxide nanoparticle (quencher), its fluorescence quenches 45 46 .…”

Section: Resultsmentioning

confidence: 99%

PEGylated silica-enzyme nanoconjugates: a new frontier in large scale separation of α-amylase

Dehnavi

Pazuki

Vossoughi

2015

Sci Rep

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High resolution is nearly lost at the expense of throughput in most conventional bioseparation methods. Nanoparticles, due to their high surface to volume ratio, are attractiveenzyme carriers, which can boost the performance of extraction manifold. Here, wereport design and application ofa method highly capable of improving the partitioning of α-amylase in aqueous two-phase system of polymer and salt. Silica nanoparticle introduced to the system acts as a bridge that connects the enzyme and polymer. Theconjugated nanoparticles form the major part of the upper phase and thus significantly enhance the protein recovery. A thorough investigation was performed on the structure of the nanoconjugatesas well as analyzing the conformational structure of the enzyme after conjugationto explore anypossible denaturation.

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Studies on the effect of AgNP binding on α-amylase structure of porcine pancreas and Bacillus subtilis by multi-spectroscopic methods

Cited by 13 publications

References 39 publications

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

PEGylated silica-enzyme nanoconjugates: a new frontier in large scale separation of α-amylase

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