Studies on the digestive enzymes of grass carp, Ctenopharyngodon idella (Val.)

Das, Kishore; Tripathi, S.D.

doi:10.1016/0044-8486(91)90005-r

Cited by 156 publications

(136 citation statements)

References 16 publications

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“…The two regions were emptied and thoroughly rinsed five times in sterile 0.9% saline in order to remove non-adherent bacteria. The two regions were separately homogenized with 10 parts of chilled 89% sodium chloride solution with due care (Das and Tripathi 1991).…”

Section: Methodsmentioning

confidence: 99%

“…Though considerable information is available regarding the intestinal microflora of homeotherms and their role in digestion, reports on the bacterial population in the gastrointestinal tract of poikilotherms, including fish, and their role in digestion are scanty. Although few reports concerning microbial enzyme production in the gastrointestinal tract of fish are available (Prejs and Blaszczyk 1977, Lindsay and Harris 1980, Lésel et al 1986, Das and Tripathi 1991, Saha and Ray 1998, Bairagi et al 2002, Saha et al 2006, information on the distribution of these enzyme-producing endosymbionts in different regions of the gut are scarce (Trust and Sparrow 1974, Sakata 1990, Mac Donald et al 1986, Ringø 1993, Ringø and and Strøm 1994.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Mondal¹,

Roy²,

Sen³

et al. 2008

Acta Icth et Piscat

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Mondal S., Roy T., Sen S.K., Ray A.K. 2008. Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish. Acta Ichthyol. Piscat. 38 (1): 1-8.Background. The information on gut microflora in fish is scanty and there is a paucity of knowledge regarding microbial enzyme activity in fish gastrointestinal tracts. Although some information is available on the enzymeproducing bacteria in fish digestive tracts, almost nothing is known about their distribution in different regions of the gut. In the present study, an attempt has been made to investigate the distribution of enzyme-producing microflora in the foregut and hindgut regions of seven culturable freshwater teleosts. Materials and Methods. Isolation and enumeration of aerobic bacterial flora in the foregut and hindgut regions of the gastrointestinal tracts of seven freshwater teleosts of different feeding habits, namely rohu, Labeo rohita; catla, Catla catla; mrigal, Cirrhinus mrigala; bata, Labeo bata; orange-fin labeo, Labeo calbasu; Nile tilapia, Oreochromis niloticus; and climbing perch, Anabas testudineus, have been carried out. Microbial culture of the gut mucosa on selected nutrient media, following the enrichment culture technique, was done for bacterial isolation. Bacterial isolates were qualitatively screened on the basis of their extracellular enzyme-producing ability. The selected strains were further quantitatively assayed for amylase, cellulase and protease activities.Results. In general, bacterial population was lower in the foregut region of all the seven species of fish examined. Amylolytic strains were present in higher densities in the foregut region of orange-fin labeo and bata (12.20 × 10 3 CFU · g -1 gut tissue and 11.50 × 10 3 CFU · g -1 gut tissue, respectively) in comparison to the hindgut region. The cellulolytic population exhibited maximum densities in the hindgut region of bata (7.20 × 10 3 CFU · g -1 gut tissue) followed by the foregut region of the same fish (5.50 × 10 3 CFU · g -1 gut tissue). Amylolytic and cellulolytic bacterial flora was not detected in both the fore and hindgut regions of climbing perch. Proteolytic bacterial flora was found in all the species of fish studied and the maximum count was observed in the hindgut region of bata (13.40 × 10 3 CFU · g -1 gut tissue), orange-fin labeo (9.00 × 10 3 CFU · g -1 gut tissue), Nile tilapia (8.30 × 10 3 CFU · g -1 gut tissue) and climbing perch (7.20 × 10 3 CFU · g -1 gut tissue). Minimum count of proteolytic bacterial flora was observed in the foregut region of all the fishes studied. Peak amylase and cellulase activities were exhibited by bacterial strains isolated from the foregut of orange-fin labeo (266.43 ± 0.15 U) and the hindgut of bata (64.01 ± 0.42 U), respectively. Maximum protease activity was exhibited by a strain isolated from the hindgut region of orange-fin labeo (44.33 ± 0.09 U), followed by the strains isolated from the hindgut regions of climbing perch (32.87 ± 0.12 U), bata (29.71 ± 0.11 U), and Nile tilapia (29.46 ± 0.11 U). C...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Mondal¹,

Roy²,

Sen³

et al. 2008

Acta Icth et Piscat

View full text Add to dashboard Cite

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“…The intestine from all fish was dissected out aseptically and homogenised with 0.89% NaCl solution (10 : 1) (Das and Tripathi 1991) The homogenate was used as inoculum. One ml of ho mogenised sai11ple (10-1 ) was spread on sterilised soybean-casein digest agar (Tryptone Soya Agar, HiMedia, India) plates and incubated at 37°C for 24 h in duplicate.…”

Section: Isolation and Selection Of Intestinal Bacterial Fl Oramentioning

confidence: 99%

Growth and survival of rohu, Labeo rohita (Hamilton) spawn fed diets supplemented with fish intestinal microflora

Ghosh¹,

Sen²,

Ray³

2002

Acta Ichthyol. Piscat.

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A 21-day feeding experiment was conducted to assess the effects of diets supplemented with fish intestinal bacteria producing extracellular enzyme on growth and survival of rohu, Labeo rohita (Hamilton) spawn (average weight 0.3 mg). Intestinal bacteria were isolated from healthy rohu fingerlings s averag � w � ight 3.51 _ ±0.31 _ g). Six isocaloric (4.24 kcal·g-) and 1somtrogenous \approximately 35% crude protein) diets (Dl-D6) were prepared containing 32% fish meal, 34% mustard oil cake, 30% rice bran, 2o/o cod liver oil, and 2% vitamin premix. Experimental diets (D2-D6) were supplemented with the isolated bacterial strain Bacillus circu lans Lr 1

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“…Although few reports concerning microbial enzyme production in the gastro Intestinal tract of fish are available [2], [3], [4], [5], [6]information on the distribution of these enzyme-producing endosymbiont sin different regions of the gutarescarce [7], [8], [9], [10], [11].…”

Section: Introductionmentioning

confidence: 99%

“…Being rich in nutrient, the environment of the digestive tract of fish confers a favorable culture environment for the microorganisms. Endogenous digestive enzymes in fish have been studied by several workers [3], [20]. In the present study, an attempt has been made to investigate the relative amount of amylase producing bacteria in the gastrointestinal (GI) tracts of two fresh water teleosts, namely the Indian major carp, Catla Catla.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Optimization of Amylase Producing Micro-Organism from Gastrointestinal Tract of Catla Catla

Chovatiya¹,

Ingale²,

Rana³

et al. 2014

IJIRSET

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ABSTRACT:Fresh water dwelling fishes have scanty information on gut microflora associated with them and there is a paucity of knowledge regarding microbial enzyme activity in fish gastrointestinal tracts. Although some of the enzymes like cellulase, proteases have been reported to be associated in the gut of fish.Amylase is one of the most important enzymes known and is of great significance and contributes of about 25% of the total enzyme market. It has a number of potential applications in food, pharmaceutical and fine chemical industries. This work describes the isolation, characterisation and optimization of amylase producing micro-organisms from gastrointestinal tract ofCatla catla. Biological production of amylase by bacterial isolate PAC4 isolated from fish gut was identified to be Bacilluscereus. The maximum amylase production was reached to 92+ 3 U/ml in 48 hours of incubation.The various physico-chemical factors for optimum amylase fermentationwere found to be 3% sodium chloride concentration,37 °C temperature and pH 7.

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Studies on the digestive enzymes of grass carp, Ctenopharyngodon idella (Val.)

Cited by 156 publications

References 16 publications

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Growth and survival of rohu, Labeo rohita (Hamilton) spawn fed diets supplemented with fish intestinal microflora

Isolation, Characterization and Optimization of Amylase Producing Micro-Organism from Gastrointestinal Tract of Catla Catla

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